1,721,163 research outputs found
Interazione dei cancerogeni presenti negli ambienti di lavoro con macromolecole biologiche
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Structural characterization of hemoglobin variants using capillary electrophoresis and fast atom bombardment mass spectrometry.
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Reactivity of antineoplastic drugs with model peptides studied by advanced mass spectrometry methodologies
No full textDevelopment of mass spectrometric approach for the characterisation of hemoglobin adducts
No full textEstrazione dei lipidi fecali e determinazione dell’acido idrossisteraico mediante gascromatografia/spettrometria di massa
No full textBiomonitoring of human exposure to methyl bromide by isotope dilution mass spectrometry of peptide adducts
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Fast atom bombardment mass spectrometric analysis of haemoglobin variants: use of V-8 protease in the identification of Hb M Hyde Park and Hb San Jose.
No full textThe characterization of two human haemoglobin variants, Hb M Hyde Park and Hb San José, by fast atom bombardment mass spectrometry is reported. The identification of the site and nature of the amino acid substitution was performed by analysis of the peptide mixture generated by proteolytic digestion of the variant beta-globin chains with V-8 protease. The use of this protease was instrumental in the unambiguous identification of the replaced residues because the tryptic map alone was unable to unequivocally locate the modification. Spectra obtained were easily interpreted and the characterization of Hb M Hyde Park (beta, 92 Hys leads to Tyr) and Hb San José (beta 7 Glu leads to Gly) was accomplished essentially by the same procedure already described for the tryptic map. These results are suggestive of alternative approaches in haemoglobin variants characterization using different proteolytic enzymes when tryptic data alone do not lead to unambiguous results
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