34 research outputs found
NAPPA as a Real New Method for Protein Microarray Generation
No full textabstract: Nucleic Acid Programmable Protein Arrays (NAPPA) have emerged as a powerful and innovative technology for the screening of biomarkers and the study of protein-protein interactions, among others possible applications. The principal advantages are the high specificity and sensitivity that this platform offers. Moreover, compared to conventional protein microarrays, NAPPA technology avoids the necessity of protein purification, which is expensive and time-consuming, by substituting expression in situ with an in vitro transcription/translation kit. In summary, NAPPA arrays have been broadly employed in different studies improving knowledge about diseases and responses to treatments. Here, we review the principal advances and applications performed using this platform during the last years
Omosessualità e diritto: cosa importa allo stato dell’orientamento sessuale dei suoi cittadini?
No full text“Use of Psycodynamic oriented Group Counselling to prevent homophobic bullying”
No full textIl capitolo descrive l'utilizzo del counselling di gruppo psicodinamicamente orientato come strumento di prevenzione a dinamiche di bullismo e bullismo omofobico
“Feel, experiment, act: an action research to prevent and contrast homophobic bullying in schools”,
No full textIl capitolo descrive un'esperienza di ricerca intervento condotta in una scuola secondaria di secondo grado di Napoli. L'obbiettivo dell'intervento era di contrastare e prevenire le molteplici forme che assume la discriminazione e la violenza scolastica, in particolare quella di marca sessista e omofobica. Nel lavoro è descritto il metodo di intervento, metodo che combinava tecniche di matrice psicodinamica e sociocognitiva
Soft Barriers for the Mitigation of Seismic Risk
No full textThis paper gives an insight on the static and dynamic performance of soft buried barriers made of a mixture of soil and a Super Absorbing Polymer (SAP) to be used for the mitigation of seismic risk. Laboratory tests were carried out on sand-SAP mixtures at different relative percentages. The results have been interpreted with reference to the peculiar properties of the two materials (sand and SAP) at the grain scale. The results of the numerical analyses indicate that the higher the SAP content, the higher the isolating effectiveness of the barrier but the worse the overall static behaviour. A balance between the two opposite needs must be found for the most effective desig
“Use of Psycodynamic oriented Group Counselling to prevent homophobic bullying: a research with teens and preadolescents”.
No full textIl contributo descrive la specificità dell'utilizzo del counselling di gruppo psicodinamicamente orientato per la prevenzione e il contrasto del bullismo e del bullismo omofobico in ambito scolastico (adolescenti e preadolescenti
Circulating and synovial antibody profiling of juvenile arthritis patients by nucleic acid programmable protein arrays.
No full textIntroduction Juvenile idiopathic arthritis (JIA) is a heterogeneous disease characterized by chronic joint inflammation of unknown cause in children. JIA is an autoimmune disease and small numbers of auto-antibodies have been reported in JIA patients. The identification of antibody markers could improve the existing clinical management of patients. Methods A pilot study was performed on the application of a high-throughput platform, nucleic acid programmable protein arrays (NAPPA), to assess the levels of antibodies present in the systemic circulation and synovial joint of a small cohort of juvenile arthritis patients. Plasma and synovial fluid from ten JIA patients was screened for antibodies against 768 proteins on NAPPA. Results Quantitative reproducibility of NAPPA was demonstrated with >0.95 intra- and inter- array correlations. A strong correlation was also observed for the levels of antibodies between plasma and synovial fluid across the study cohort (r=0.96). Differences in the levels of 18 antibodies were revealed between sample types across all patients. Patients were segregated into two clinical subtypes with distinct antibody signatures by unsupervised hierarchical cluster analysis. Conclusions NAPPA provides a high-throughput quantitatively reproducible platform to screen for disease specific autoantibodies at the proteome level on a microscope slide. The strong correlation between the circulating antibody levels and those of the inflamed joint represents a novel finding and provides confidence to use plasma for discovery of autoantibodies in JIA, thus circumventing the challenges associated with joint aspiration. We expect that autoantibody profiling of JIA patients on NAPPA could yield antibody markers that can act as criteria to stratify patients, predict outcomes and understand disease etiology at the molecular level
Real-Time Estimation of Rt for Supporting Public-Health Policies Against COVID-19
No full textIndexación ScopusIn the absence of a consensus protocol to slow down the spread of SARS-CoV-2, policymakers need real-time indicators to support decisions in public health matters. The Effective Reproduction Number (Rt) represents the number of secondary infections generated per each case and can be dramatically modified by applying effective interventions. However, current methodologies to calculate Rt from data remain somewhat cumbersome, thus raising a barrier between its timely calculation and application by policymakers. In this work, we provide a simple mathematical formulation for obtaining the effective reproduction number in real-time using only and directly daily official case reports, obtained by modifying the equations describing the viral spread. We numerically explore the accuracy and limitations of the proposed methodology, which was demonstrated to provide accurate, timely, and intuitive results. We illustrate the use of our methodology to study the evolution of the pandemic in different iconic countries, and to assess the efficacy and promptness of different public health interventions. © Copyright © 2020 Contreras, Villavicencio, Medina-Ortiz, Saavedra and Olivera-Nappa.
Author keywordshttps://www.frontiersin.org/articles/10.3389/fpubh.2020.556689/ful
