49 research outputs found
A sensitive electrochemical nitrate sensor based on polypyrrole coated palladium nanoclusters
No full textAbstract not available.M.R. Mahmoudian, Y. Alias, W.J. Basirun, Pei MengWoi, Farid Jamali-Sheini, M. Sookhakian, M. Silakhor
Evaluation and Optimization of Lipofectamine 3000 Reagents for Transient Gene Expression in KYSE-30 Esophagus Cancer Cell Line
No full textBackground and Aim: Transfection of DNA/RNA sequence into eukaryotic cells has a major effect on scientific studies. Various methods are used to transfer the DNA/RNA sequence into cells, such as lipid-based carriers as the available and easy procedure. Transfection with cationic lipid liposome is introduced as a simple and efficient procedure for monitoring the DNA/RNA sequence through gene function analysis, including fluorescence imaging RNA and protein expression. This study aimed to investigate the transfection efficiency and cell death through GFP expression in human esophageal squamous cell carcinoma (ESCC) cell line KYSE-30 using Lipofectamine 3000 reagent. Methods: The pCDH-513b plasmid DNA was transfected into KYSE-30 cells using Lipofectamine 3000 in different concentrations of the plasmid DNA and reagent. The transfection efficiency was evaluated by fluorescence microscope and flow cytometry analysis to determine the percentage of GFP-expressing cells. Moreover, the viability and death of transfected KYSE-30 cells were evaluated using a trypan blue exclusion assay. Results: The transfection efficiency of KYSE-30 with Lipofectamine 3000 was increased with higher plasmid DNA concentration and a lower amount of Lipofectamine 3000 reagent. The Optimized concentration of 1.5 µg plasmid DNA and volume of one µl of lipofectamine 3000 reagents were identified for 95% transfection efficiency in the KYSE-30 cell line. The viability and death of transfected cells were 43% and 58% after transfection, respectively. Conclusion: The results indicated that Lipofectamine 3000 might not be suitable for transfection in KYSE-30 cells due to increased cell death.
*Corresponding Author: Mohammad Reza Abbaszadegan; Email: [email protected]
Please cite this article as: Mahmoudian RA, Farshchian M, Abbaszadegan MR. Evaluation and Optimization of Lipofectamine 3000 Reagents for Transient Gene Expression in KYSE-30 Esophagus Cancer Cell Line. Arch Med Lab Sci. 2019;5(4):1-9. https://doi.org/10.22037/amls.v5i4.3108
Synthesis of polypyrrole/Ni-doped TiO 2 Nanocomposites (NCs) as a protective pigment in organic coating
No full textThis study reports the synthesis of polypyrrole/Ni-doped TiO 2 nanocomposites (NCs) as a protective pigment in organic coatings. Polypyrrole/Ni-doped TiO 2 NCs were prepared by in situ chemical oxidative polymerization of pyrrole monomer in the presence of Ni-doped TiO 2 nanoparticles (NPs) with ammonium persulfate (APS) as oxidant. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) result show a core-shell structure of the pigments. The XRD results indicate that the average crystalline size of Ni-doped TiO 2 NPs is larger than TiO 2 NPs while the sizes of polypyrrole/TiO 2 NCs and polypyrrole/Ni-doped TiO 2 NCs were 93.46 ± 0.06 and 26.16 ± 0.06 nm respectively. Hence the thickness of the shell in the core-shell incorporating the Ni-doped/TiO 2 NPs was very thin and the area of synthesized PPy is increased. The electrochemical impedance spectroscopy (EIS) results show that increasing the area of synthesized polypyrrole in the presence of Ni-doped-TiO 2 NPs can increase its ability to interact with the ions liberated during the corrosion reaction of steel in the presence of NaCl. © 2010 Elsevier B.V. All rights reserved
Synthesis and characterization of poly(N-methylpyrrole)/TiO2 composites on steel
No full textPoly(N-methyl pyrrole) coating was successfully electrodeposited on steel substrates in mixed electrolytes of dodecyl benzene sulphonic acid (DBSA) with oxalic acid in the absence and the presence of TiO2 nanoparticles (NPs). The morphology and compositions were characterized by Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR), Energy-Dispersive X-ray spectroscopy (EDX). X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM) were used to calculate the size of nanoparticles. Electrode/polymer/electrolyte system was studied by Electrochemical Impedance Spectroscopy (EIS). The FESEM micrographs suggest that the incorporation of TiO2 nanoparticles affects the morphology of the film significantly and makes the TiO2 to be loosely piled up with PMPy. The results of EIS showed that synthesized PMPy in the presence of TiO2 NPs increases and decreases the R-po and C-c of the coating respectively. The increase of the area of synthesized PMPy in the presence of nanoparticles can increase its ability to interact with the ions liberated during the corrosion reaction of steel in NaCl solution. (C) 2010 Elsevier B.V. All rights reserved
Electrodeposition Of (Pyrrole-Co-Phenol) On Steel Surfaces In Mixed Electrolytes Of Oxalic Acid And DBSA
No full textPoly (pyrrole-co-phenol) (co-PyPh) was synthesised using cyclic voltammetry in the mixture of dodecyl benzene sulphonic acid (DBSA) with oxalic acid solution on steel electrodes The morphology and compositions were characterized by Field Emission Scanning Electron Microscopy (FESEM) Fourier Transform Infrared Spectroscopy (FTIR) techniques and Energy-dispersive X-ray spectroscopy (EDX) Electrode/co-PyPh/electrolyte system was studied in 0 1 M NaCl solutions by Electrochemical Impedance Spectroscopy (EIS) The Warburg impedance observed in mid frequency region is an evidence of the effective barrier behaviour of coating The results of EDX indicate that the content of doped sulphur can influence the morphology of co-PyPh Three dissimilar morphologies of co-PyPh and different amount of doped sulphur in co-PyPh are observed The existence of phenol monomer leads to a decrease in the amount of doped sulphur in co-PyPh compared with synthesised polypyrrole (PPy) in the same environment (c) 2010 Elsevier B V All rights reserve
The electrical properties of a sandwich of electrodeposited polypyrrole nanofibers between two layers of reduced graphene oxide nanosheets
No full textA sandwich of polypyrrole (PPy) nanofibers between two electrodeposited reduced graphene oxide (RGO) layers was prepared by an electrochemical method. The structure and compositions were characterised by field emission scanning electron microscopy (FESEM) and Fourier transform infrared (FT-IR) spectroscopy. The electrical properties were studied by electrochemical impedance spectroscopy (EIS). The FESEM results confirmed synthesis of a sandwich structure with PPy nanofibers between two electrodeposited RGO layers (RGO/PPy/RGO). The mean width of PPy nanofibers was approximately 76 +/- 16 nm. The EIS results showed that the charge transfer resistance of the coated glassy carbon electrode (GCE) with a sandwich structure of RGO/PPy/RGO was significantly decreased in comparison with coated GCE with PPy nanofibers. This was because the RGO with many small band gaps is more favourable for electron conduction. The comparison of EIS results shows that diffusion of active species through the RGO layers is increased in the presence of the second layer of RGO. The simulation results indicate a high interfacial capacitance (C-dl) of 2170.60 mu F cm(-2) for the coated GCE with RGO/PPy/RGO compared with 360.77 mu F cm(-2) for coated GCE with PPy/RGO. This difference in capacitance can be attributed to the highly accessible specific surface area of graphene and its high efficiency towards electrolyte ion absorption. (C) 2012 Elsevier Ltd. All rights reserved
Comparison of corrosion protection between double strands of polyaniline and poly-o-anisidine with poly(Acrylic Acid-Co-Acryl Amide) on steel
No full textDouble strands of polyaniline (PAn) and poly(o-anisidine) (POAn) with poly(acrylic acid-co-acryl amide) (PAA-co-AA) were successfully prepared on steel as undercoating, by immersion of the pretreated surfaces into a PAn: (PAA-co-AA) and POAn: (PAA-co-AA) saturated DMF solution separately. The undercoatings formed on the steel were characterized by Fourier transform infrared spectroscopy (FTIR) techniques. A commercial paint (Nippon Paint, nonadded Chrome) was used as topcoating. Electrode/electroactive polymer/paint/electrolyte system was studied by electrochemical impedance spectroscopy (EIS). The EIS studies show that during the first 18 days immersion time in 3.5% solution of NaCl, paint/PAn coating has better corrosion resistance than paint/POAn coating while in the final week of immersion time, the pore resistance (R-po) and coating capacitance (C-c) of paint/POAn are higher and lower than the paint/PAn, respectively
Effect of narrow diameter polyaniline nanotubes and nanofibers in polyvinyl butyral coating on corrosion protective performance of mild steel
No full textThis study shows the effect of narrow diameter polyaniline (PAn) nanotubes and nanofibers in polyvinyl butyral coating on corrosion protective performance of mild steel. The PAn nanotubes and nanofibers were synthesised by chemical oxidative polymerisation in the presence of dodecylbenzene sulfonic acid (DBSA) as a bulky dopant acid. The molar ratios of monomer to DBSA used for the nanotube and the nanofiber synthesis are 1:1 and 4:1, respectively. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) results confirm the narrow diameter range of 180-230 nm for the PAn nanotubes and nanofibers. Electrochemical impedance spectroscopy (EIS) confirms that the resistance of the coating containing PAn nanotubes is three times higher than the coating containing PAn nanofibers after 30 days of immersion. This effect can be explained because of the higher surface area of the nanotubes compared to nanofibers with the same mass. This difference increases the ability of PAn to interact with the ions liberated during the corrosion of the steel and increases the rate of cathodic reduction of oxygen on the surface of PAn. © 2012 Published by Elsevier B.V. All rights reserved
Studies on the Effects of the Potential Sweep Rates and pH on the Corrosion Rate of Polyaniline Coated Steel
No full textPolyaniline (PAni) film was successfully electrodeposited on the surface of steel substrates in oxalic acid electrolyte by cyclic voltammetry at different potential sweep rates (dE/dt) (10, 20 and 30 mVs-1) and different pH values (1.55, 2.55 and 3.55). The coated steel with PAni was characterized by Field Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared Spectroscopy (FTIR) techniques. The corrosion resistance of the electrodeposited layers was evaluated by potentiodynamic polarization in 0.1 M NaCl aqueous solution. The results showed that with increasing pH and sweep rate the amount of corrosion rate decreased and increased respectively. Moreover, the correlation between variation of anodic charge (Qa) with pH and sweep rate has been evaluated. The variation of anodic charge confirmed that the growing rate of polymer decreased with increasing sweep rate and pH. The result of potentiodynamic polarization showed that the coated steel with synthesized PAni in pH 3.5 and sweep rate 0.01Vs-1 had better protective properties against corrosion.</jats:p
