9 research outputs found
Collection and characterization of grapevine genetic resources (Vitis vinifera) in the Holy Land, towards the renewal of ancient winemaking practices
No full textThe importance and extent of wine consumption in all life aspects at the Holy Land is well documented. The Muslim influence in this region led to the abandonment of winemaking practices, and possible loss of indigenous wine varieties. Here we present a country wide collection of the local grapevine population including wild and cultivated forms, and its characterization by genetic, ampelographic and enological methods. The ampelographic analysis shows clear differences between Sativa and Sylvestris groups in flower, leaf and cluster parameters, and that most Sativa belong to proles orientalis. Genetic population analysis was conducted by analyzing 22 common SSR markers, determining first the unique genotypes, and internally assessing the population’s structure, showing the existence of two distinct Sativa and Sylvestris populations, and a third mixed one. Likewise, the relationship between the Israeli grapevine population and grapevine populations in Europe and parts of Asia was investigated, showing that the Israeli Sativa and Sylvestris populations cluster closely together, suggesting a common genetic source. Lastly, the enological characteristics of selected Sativa and Sylvestris genotypes are presented, demonstrating their potential for quality wine production. This research significantly contributes toward the re-establishment of indigenous and traditional local grapevine varieties into the modern international wine industry
Aspirin or enoxaparin thromboprophylaxis for newly-diagnosed multiple myeloma patients treated with lenalidomide.
No full textLenalidomide plus dexamethasone is effective in the treatment of multiple myeloma (MM) but is associated with an increased risk of venous thromboembolism (VTE). This prospective, open-label, randomized substudy of a phase 3 trial compared the efficacy and safety of thromboprophylaxis with low-dose aspirin (ASA) or low-molecular-weight heparin (LMWH) in patients with newly diagnosed MM, treated with lenalidomide and low-dose dexamethasone induction and melphalan-prednisone-lenalidomide consolidation. Overall, 342 patients who did not have clinical indications or contraindications to antiplatelet or anticoagulant therapy were randomly assigned to receive ASA 100 mg/d (n = 176) or LMWH enoxaparin 40 mg/d (n = 166). The incidence of VTE was 2.27% in the ASA group and 1.20% in the LMWH group. Compared with LMWH, the absolute difference in the proportion of VTE was 1.07% (95% confidence interval, -1.69-3.83; P = .452) in the ASA group. Pulmonary embolism was observed in 1.70% of patients in the ASA group and none in the LMWH group. No arterial thrombosis, acute cardiovascular events, or sudden deaths were reported. No major hemorrhagic complications were reported. In previously untreated patients with MM receiving lenalidomide with a low thromboembolic risk, ASA could be an effective and less-expensive alternative to LMWH thromboprophylaxis. This study was registered at www.clinicaltrials.gov as #NCT00551928. © 2012 by The American Society of Hematology
Aspirin or enoxaparin thromboprophylaxis for patients with newly diagnosed multiple myeloma treated with lenalidomide
No full textLenalidomide plus dexamethasone is effective in the treatment of multiple myeloma (MM) but is associated with an increased risk of venous thromboembolism (VTE). This prospective, open-label, randomized substudy of a phase 3 trial compared the efficacy and safety of thromboprophylaxis with low-dose aspirin (ASA) or low-molecular-weight heparin (LMWH) in patients with newly diagnosed MM, treated with lenalidomide and low-dose dexamethasone induction and melphalan-prednisone-lenalidomide consolidation. Overall, 342 patients who did not have clinical indications or contraindications to antiplatelet or anticoagulant therapy were randomly assigned to receive ASA 100 mg/d (n = 176) or LMWH enoxaparin 40 mg/d (n = 166). The incidence of VTE was 2.27% in the ASA group and 1.20% in the LMWH group. Compared with LMWH, the absolute difference in the proportion of VTE was 1.07% (95% confidence interval, -1.69-3.83; P = .452) in the ASA group. Pulmonary embolism was observed in 1.70% of patients in the ASA group and none in the LMWH group. No arterial thrombosis, acute cardiovascular events, or sudden deaths were reported. No major hemorrhagic complications were reported. In previously untreated patients with MM receiving lenalidomide with a low thromboembolic risk, ASA could be an effective and less-expensive alternative to LMWH thromboprophylaxis. This study was registered at www.clinicaltrials.gov as #NCT00551928
Erratum: Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells
No full textAntigen Is Required for Maturation and Activation of Pathogenic Anti-DNA Antibodies and Systemic Inflammation
No full textAbstract
Systemic lupus erythematosus is an autoimmune disease characterized by autoantibodies and systemic inflammation that results in part from dendritic cell activation by nucleic acid containing immune complexes. There are many mouse models of lupus, some spontaneous and some induced. We have been interested in an induced model in which estrogen is the trigger for development of a lupus-like serology. The R4A transgenic mouse expresses a transgene-encoded H chain of an anti-DNA Ab. This mouse maintains normal B cell tolerance with deletion of high-affinity DNA-reactive B cells and maturation to immunocompetence of B cells making nonglomerulotropic, low-affinity DNA-reactive Abs. When this mouse is given estradiol, normal tolerance mechanisms are altered; high-affinity DNA-reactive B cells mature to a marginal zone phenotype, and the mice are induced to make high titers of anti-DNA Abs. We now show that estradiol administration also leads to systemic inflammation with increased B cell-activating factor and IFN levels and induction of an IFN signature. DNA must be accessible to B cells for both the production of high-affinity anti-DNA Abs and the generation of the proinflammatory milieu. When DNase is delivered to the mice at the same time as estradiol, there is no evidence for an abrogation of tolerance, no increased B cell-activating factor and IFN, and no IFN signature. Thus, the presence of autoantigen is required for positive selection of autoreactive B cells and for the subsequent positive feedback loop that occurs secondary to dendritic cell activation by DNA-containing immune complexes.</jats:p
Effect of Combination Ultra Q10 and L-Carnitine on the Clinical Course and Mitochondrial Function of Patients with Myelodysplastic Syndrome (MDS)
No full textForever Young? Late Shoot Pruning Affects Phenological Development, Physiology, Yield and Wine Quality of Vitis vinifera cv. Malbec
No full text‘Malbec’ grapevines commonly have high yield, thus intermittently negatively affecting wine quality parameters. Here, we describe the use of late shoot pruning (LSP) practice applied for wine quality improvement. We examined the effect of timing of LSP on ‘Malbec’ vines grown in Mediterranean conditions during three consecutive seasons (2016–2018) in Israel. The timing of LSP treatment applications (applied one, two and three weeks after bud break) were compared with cluster-thinned, winter-pruned vines (WP + T) and standard winter pruning (WP). The LSP practice postponed bud break of target buds but did not have a temporal effect on the onset of veraison. Midday stem water potential was less negative and stomatal conductance and net CO2 assimilation rate were higher in the LSP vines. This practice led to a substantial reduction in the number of clusters and crop yield. Finally, wine quality was positively affected by applying LSP treatment. Performing the inexpensive LSP treatment at the precise timing after bud burst was found to save labor, decrease crop yield and improve grape and wine parameters. LSP application should be considered in adequate varieties as a significant tool for the enhanced effectiveness of vine growing in warm regions
Melphalan/prednisone/lenalidomide (MPR) versus high-dose melphalan and autologous transplantation (MEL200) in newly diagnosed multiple myeloma (MM) patients: A phase III trial.
No full textProducción de vino de corozo chiquito (Bactris minor/guinensis) a partir de diferentes sustratos teniendo en cuenta la normatividad vigente en Colombia.
No full textAnte la tendencia de diversificar la producción enológica en contextos tropicales y valorizar especies frutales nativas como el corozo chiquito (Bactris minor/guinensis), reconocido por su color violáceo variable y su riqueza en antocianinas con efectos terapéuticos. Esta investigación evaluó el efecto de distintos sustratos y sus concentraciones iniciales en la producción de vino a partir de esta fruta. Se emplearon tres sustratos (sacarosa, melaza y azúcar invertido) en concentraciones de 12,5°, 18,8° y 25°Brix. La caracterización del fruto en seis subregiones del departamento de Córdoba evidenció diferencias agroclimáticas significativas. El Medio Sinú presentó condiciones favorables para la fermentación, destacando por su elevada concentración de antioxidantes (192,74 ± 1,04 µmol ET/100g), sólidos solubles (21,5 ± 3,5 °Brix) y densidad (1,07 ± 0,010 g/cm³); la Región Costanera sobresalió por su contenido de polifenoles (785,67 ± 1,4 mg GAF/g). El modelamiento cinético indicó que la melaza a 25°Brix mostró los parámetros más estables (µmax = 0,0196 h⁻¹; Ks = 62,5 g/L), mientras que la sacarosa a 12,5°Brix alcanzó la mayor µmax (0,0244 h⁻¹), aunque con menor rendimiento. El azúcar invertido evidenció mayor variabilidad cinética, posiblemente por limitaciones nutricionales o estrés osmótico. La melaza a 25°Brix generó el mejor rendimiento fermentativo, con 11,90 ± 2,3 % v/v de etanol y 1130 ± 2,5 mg GAF/g de polifenoles. Estos resultados destacan el potencial del corozo como materia prima alternativa para vinos tropicales y respaldan la necesidad de actualizar la legislación enológica actual de Colombia, incorporando criterios diferenciales para vinos elaborados a partir de frutas tropicales, se recomienda promover marcos normativos específicos que reconozcan la autenticidad y valor cultural de estos productos, garanticen su inocuidad y calidad, fomentando su inclusión en mercados formales, impulsando así la bioeconomía y el desarrollo agroindustrial en regiones donde históricamente la viticultura no era considerada viable.In response to the growing trend of diversifying oenological production in tropical environments and valorizing native fruits such as Bactris minor/guinensis commonly known as corozo chiquito and notable for its anthocyanin-rich pigmentation. This study evaluated the influence of different substrates (sucrose, molasses, and inverted sugar) at three concentrations (12.5°, 18.8°, and 25° Brix) on the fermentation performance of this fruit. Fruit characterization in six subregions of Córdoba, Colombia, revealed significant agroclimatic variation. The Medio Sinú region showed the most favorable profile for fermentation, with elevated antioxidant content (192.74 ± 1.04 µmol TE/100g), high soluble solids (21.5 ± 3.5 °Brix), and density (1.07 ± 0.010 g/cm³). Conversely, the Coastal Region had the highest polyphenol content (785.67 ± 1.4 mg GAE/g). Kinetic modeling indicated that molasses at 25° Brix provided the most stable fermentation parameters (µmax = 0.0196 h⁻¹; Ks = 62.5 g/L). Although sucrose at 12.5° Brix showed a higher specific growth rate (µmax = 0.0244 h⁻¹), it resulted in lower yields. Inverted sugar exhibited greater kinetic variability, possibly due to osmotic stress or limited nutrient availability. Fermentation with molasses at 25° Brix yielded the highest ethanol content (11.90 ± 2.3 % v/v) and polyphenol concentration (1130 ± 2.5 mg GAE/g). These findings affirm corozo’s potential as a viable raw material for tropical wine production and highlight the need to update Colombian oenological legislation. It is proposed that new regulatory frameworks be adopted to recognize the authenticity, safety, and cultural value of tropical fruit-based wines, promoting formal market integration and supporting bioeconomic and agro-industrial development in non-traditional viticultural regions.INTRODUCCIÓN 152 REVISIÓN DE LITERATURA 162.1 MARCO TEORICO 192.1.1. Agroindustria y desarrollo sostenible 192.1.2. El corozo chiquito o uvita de lata (Bactris minor/guineensis) 202.1.3. Cadena de valor 212.1.4. Producción 222.1.5. Mercado 232.1.6. Perspectivas de Crecimiento 232.1.7. Fermentación 242.1.8. Vino de frutas 262.1.9. Edulcorantes y azúcares adicionados o añadidos 273 OBJETIVOS 293.1. Objetivo General 293.2. Objetivos Específicos 304 METODOLOGÍA 304.1 Caracterización fisicoquímica del fruto de corozo chiquito (Bactris minor/guinensis) según la región de origen del Departamento de Córdoba. 304.1.1 Materia prima 314.1.2 Acidez total titulable. 314.1.3 Cenizas 314.1.4 Densidad 314.1.5 Determinación de actividad antioxidante por DPPH 314.1.6 Humedad 314.1.7 Índice de madurez 324.1.8 pH 324.1.9 Sólidos solubles (°Brix) 324.2.1 Elaboración del vino 324.2.2 Curado de los fermentadores 334.2.3 Chaptalización 334.2.4 Pasteurización del mosto 334.2.5 Fermentación 344.2.6 Trasiego 344.2.7 Maduración 344.2.8 Clarificación 344.2.9 Envasado 354.3 Evaluación de parámetros cinéticos en la fermentación alcohólica 354.3.1 Diseño Experimental. 354.4 Determinación de las propiedades fisicoquímicas, microbiológicas y sensoriales del vino de corozo chiquito. 364.4.1 Propiedades fisicoquímicas 364.4.2 Acidez Volátil 364.4.3 Azúcares reductores 364.4.4 Color 364.4.5 Grados alcoholimétricos. 374.4.6 Propiedades microbiológicas 374.4.7 Análisis sensorial 375 RESULTADOS 395.1 Caracterización fisicoquímica del fruto de corozo chiquito (Bactris minor/guinensis) según la región de origen en el departamento de Córdoba. 395.2 Desarrollo de una metodología aplicada en el proceso de la fermentación y evaluación de sus parámetros cinéticos para obtener vino de corozo chiquito acorde a lo establecido por la normatividad nacional vigente. 465.2.1 Cinética microbiana 465.2.2 Evaluación de los parámetros cinéticos. 555.2.3 Rendimiento de conversión sustrato/producto bajo diferentes condiciones fermentativas 585.2.4 Maduración 605.3 Evaluación de las propiedades fisicoquímicas, microbiológicas, sensoriales y capacidad antioxidante de los vinos obtenidos a partir del corozo chiquito (Bactris minor/ guinensis). 645.3.1 Propiedades fisicoquímicas de los vinos obtenidos a partir del corozo chiquito (Bactris minor) 645.3.2 Colorimetría 665.3.3 Análisis sensorial 685.3.4 Propiedades microbiológicas 705.3.5 Capacidad antioxidante 71CONCLUSIONES 73RECOMENDACIONES 75BIBLIOGRAFIA 76MaestríaMagíster en Ciencias AgroalimentariasTrabajos de Investigación y/o Extensió
