1,720,988 research outputs found
ESTABLISHMENT AND OPTIMIZATION OF THE CHROP APPROACH, COMBINING CHIP AND MS-BASED PROTEOMICS, FOR THE CHARACTERIZATION OF THE CHROMATOME AT DISTINCT FUNCTIONAL DOMAINS
Full text linkChromatin is a highly dynamic, well-structured nucleoprotein complex of DNA and proteins that controls virtually all DNA-transactions. Chromatin dynamicity is regulated at specific loci by the presence of various associated proteins, histones post-translational modifications, histone variants and DNA methylation. Until now the characterization of the proteomic component of chromatin domains has been held back by the challenge of enriching distinguishable, homogeneous regions for the subsequent mass spectrometry analysis and thus remains a very attractive unachieved goal. I contributed in this direction developing and optimizing a proteomic strategy that combines chromatin immunoprecipitation with quantitative proteomics based on stable isotope labeling by amino acids in cell culture to identify known and novel histone modifications, variants and complexes that specifically associate with silent and active chromatin domains. This chromatin proteomics strategy revealed unique functional interactions among various chromatin modifiers, thus suggesting new regulatory pathways, such as an heterochromatin-specific modulation of DNA damage response involving H2A.X and WICH, both enriched in silent domains. Chromatin proteomics expands the arsenal of tools for deciphering how all the distinct protein components act together to enforce a given region-specific chromatin status
Analisi di contesto per la gestione di gravidanza, parto e puerperio, in un ospedale del Nord Uganda
No full textINTRODUZIONE: Ogni giorno, nel mondo, 800 donne perdono la vita per complicanze dovute a gravidanza e parto. Quasi la totalità di queste vivono in paesi del sud del pianeta. Uno dei fattori legati alla mortalità materna al parto è la mancata assistenza da parte dei professionisti sanitari. L’Uganda fa parte di questi paesi.
OBBIETTIVO: Osservare l’assistenza ostetrica presso l’ospedale St. Mary Lacor del Nord Uganda
MATERIALE E METODI: attraverso una permanenza partecipante nel mese di luglio 2015, è stato possibile raccogliere dati in merito alla gestione di gravidanza, parto e puerperio da parte delle ostetriche. Inoltre è stata raccolta un’intervista a un medico responsabile per comprendere l’accompagnamento ambulatoriale alla gravidanza.
RISULTATI: Le attività osservate e il materiale raccolto è stato organizzato secondo: le aree operative, l’utenza, i professionisti all’opera e il materiale cartaceo.
DISCUSSIONE E CONCLUSIONI: per quanto possibile, la realtà ugandese è stata confrontata con la realtà italiana della Clinica Mangiagalli, avente un bacino d’utenza simile. Ciò ha permesso di identificare comunanze e differenze e di analizzare criticamente gli elementi dell’assistenza ostetrica, nei rispettivi contesti.
PROPOSTE OPERATIVE: dal lavoro svolto è emerso che il St. Mary’s Lacor hospital rappresenta una struttura d’avanguardia nel contesto ugandese, questa realtà può essere sostenuta da collaborazione reciproca, che sviluppi “best practice”, apprendimento e formazione continua
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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