1,720,996 research outputs found
COST-EFFECTIVE USE OF MOLECULAR MARKERS IN THE PRACTICAL RESOLUTION OF COMMON HORTICULTURAL CHALLENGES
Full text linkGenetic molecular markers (DNA markers) represent genetic differences between individual organisms or species placed directly into DNA sequence. They are widely used as powerful scientific instruments to accomplish different tasks, from genes mapping to forensic discrimination. The tremendous advance in DNA genotyping tools has lead to the development of impressive high- throughput technologies, such as Next Generation Sequencing platforms, that may revolutionize horticulture research and applications. However the cost of such technologies not always make them the most rationale approach, particularly when working on minor crop species or with large number of samples. The present work aims to the exploring a multi-purpose and cost-effective use of different kinds of molecular markers, for assisting fruit tree plants breeding and valorization. For this scope, three cases of study were presented, spanning from cultivar discrimination and phylogeny reconstruction to marker assisted selection (MAS) for Sharka resistance.
D.NA markers such as SSR and AFLP, were successfully used to discriminate the ‘common’ Chinotto from ‘Chinotto di Savona’, an uninvestigated traditional Citrus species cultivated in Liguria (italy) that is gaining increasing interest for the production of high-quality niche food and beverages. New polymorphisms on candidate genes, that could explain some of observed differences between the two accessions, were suggested.
SSR markers were used for the first time to the large-scale application of MAS on apricot (Prunus armeniaca) to boost the conventional breeding programmes. They were found new resistant breeding selections against the most important viral disease of stone fruits, Sharka, caused by Plum Pox Virus (PPV). Novel candidate accessions were also characterized for PPV-resistance, enriching and complementing the apricot germplasm available for breeding. Moreover the number of significant markers required for this task was reduced from seven to two, decreasing the overall cost, in terms of time and resources, usually required for the conventional breeding programmes.
A further reduction of resources for the application of MAS in apricot was achieved developing new SNP markers linked to Sharka resistance, and able to be screened using fluorescence on Real Time PCR machine with or without High Resolution Melting (HRM) technology.
The performed works demonstrate that the correct choice of molecular instruments together with the implementation of new techniques could easily led to cost-effective, time-saving, and reliable results even without the facility and resources reserved for main crops research and applications
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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