1,721,302 research outputs found
Il sistema SCAN, uno strumento per rendere confrontabili le descrizioni e le classificazioni delle condizioni psicopatologiche
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Pathophysiology of schizophrenia and the neurodevelopmental hypothesis: evidence from an animal model
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One hundred years of X-ray diffraction, 50 years of structural biology
No full textSince the pioneering diffraction experiments led by Max von Laue in 1912, X-ray crystallography has progressed with a spectacular pace, opening research, and application perspectives in fields such as materials science, chemistry, mineralogy, environmental sciences, biotechnology and biomedicine. Such developments have been supported by discoveries in both the theoretical and experimental branches of science that granted the different communities access to specific investigation tools. In the biological field the highest challenge presented to X-ray crystallography has been linked to the study of proteins and nucleic acids, i.e., macromolecular structures composed of thousands of atoms, often available for the analyses in microscopic crystals of low intrinsic order. To support the development of macromolecular crystallography several complementary techniques had to be devised, such as methods in biochemistry and molecular biology, but also progresses in synchrotron X-ray sources. As the contemporary crystallographic methods allow facing very complex protein structures, in relatively short response times, the interest of corporate research for applied crystallography has grown substantially in the last two decades. In particular, current approaches to drug discovery and optimization rely on rational principles that stem from crystallographic investigations on protein/lead complexes, and on cyclic optimization of their chemical synthesis. Notably, the United Nations declared 2014 the International Year of Crystallography in consideration of the fundamental role played by crystallography for the growth of scientific knowledge
The H2A/H2B-like histone-fold domain proteins at the crossroad between chromatin and different DNA metabolisms
No full textCore histones are the building block of chromatin and among the most highly conserved proteins in eukaryotes. The related "deviant" histones share the histone-fold domain, and serve various roles in DNA metabolism. We provide here a structural and functional outlook of H2A/H2B-like deviant histones in transcription, replication and remodeling
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Il paziente portatore di patologia neoplastica nel cavo orale: approccio multidisciplinare
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Determination of aminoethylcysteine ketimine decarboxylated dimer in human plasma and cultured cells by high-performance liquid chromatography with electrochemical detection
No full textAminoethylcysteine ketimine decarboxylated dimer (AECK-DD) is a natural compound with antioxidant properties of a new family of sulfur-containing amino acids. It has been detected in human urine and plasma, in mammalian cerebellum and, more recently, in dietary vegetables. In the present study, a simple, highly sensitive method using a high-performance liquid chromatography system with electrochemical detection (ECD) has been developed. The method showed excellent precision and accuracy. It has been found to be about 100-fold more sensitive than gas chromatographic method and 2000-fold more sensitive in respect to the liquid chromatography method with UV detection. The method showed the required features of specificity and sensitivity to detect aminoethylcysteine ketimine decarboxylated dimer in human plasma and in cultured cells after in vitro supplementation. (C) 2003 Elsevier B.V. All rights reserved
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