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    A novel biopsy technique for lesions involvinginterproximal soft tissues

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    Background: Biopsy of soft tissues around teeth can cause periodontal deficiency with several side effects. When the neoformation involves frontal areas biopsy often induces an aesthetic impairment, particularly critical when the interdental papilla is entailed. Description of the procedure: A new surgical approach to treat lesions involving interdental tissues is described. The flap design is characterized by a sub-marginal scalloped incision, possibly confined into the keratinized tissue at the vestibular side. The incision starts 1 mm above the lesion, in the midline between the two confining teeth. With a parabolic shape, the incision reaches the gingival margin at the distal line angle of the distal tooth. Hence, another parabolic incision is performed connecting the starting point with the marginal mesial line angle of the mesial tooth. After this step, two options are applied depending on the lesion extension. When the lesion is confined coronally to the line connecting the gingival margin zeniths of the two adjacent teeth, oblique linear incisions are performed till to the gingival margin zeniths. Otherwise, when the lesion extends beyond this line, two parabolic incisions are per-formed on both adjacent teeth, till to the distal and mesial line angle respectively. Specific anatomic measurements rule up the incisional design. For the palatal/lingual side, the incision follows the principles of a general excisional procedure. Intrasulcular incisions are then made and the lesion with surrounding tissues are collected for examination. Both full-thickness and split-thickness are used for the buccal flap elevation. De-epithelized the adjacent anatomic papillae, two sling sutures are used to stabilize the entire flap to the most feasible coronal position. Generally single sutures are used for the palatal side and the eventual graft. Outcomes: All 9 cases treated so far have ever shown an esthetically satisfying outcome without recurrence or adverse events. Conclusions: This technique allows clinician to restore gingival health preserving aesthetics and function

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Is nutrition a risk or a protective factor for periodontal disease?

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    Background and Aim: Nutrition is a widely discussed topic in dentistry. In periodontology have been reported how vitamins, antioxidants (also in fruit) and folic acid could play a protective role from inflammation. The aim of this study was to evaluate if nutritional habits of periodontal patients could have influenced the severity of their periodontal status and its bacterial load.Methods: Thirty-four periodontal patients that underwent to a full periodontal examination (periodontology division - University of Bologna) and diagnosis with a moderate to severe periodontitis were included in the study. Patients with antibiotic therapy in the last 6 months and pregnant women were excluded. All were questioned about their previous 6 months food habits using the Food FrequencyTest (FFQ). The FFQ was reduced and integrated collecting the following data: body weight and height, waist circumference, smoking, diabetes and vitamins intake. Participants were divided according to the main variable, daily fruit consumption, in two groups of 17 subjects, and compared by a multilevel analysis to the periodontal parameter recovered from the initial exams. FFQ, probing depth (PPD),clinical attachment level (CAL), bleeding on probing (BoP), plaque index (P.I. O'Leary), exudate, microbial site specific real-time PCR test(Biomolecular Diagnostic®) results and percentage of mean bone level were used.Results: The multilevel statistical analysis did not report any significant results regarding food habits, periodontal parameters and microbial charges for both groups. Particularly no statistical differences emerged for PPD, CAL, BoP, and exudate. Even bacterial charges of the periodontal pathogens investigated, with a mean value associated to moderate to severe periodontitis, did not show any statistical difference. Conclusions: Form these results did not emerge any role of nutrition on the periodontal parameters collected, suggesting a marginal role subordinate to more influential variables. Further investigations including healthy, gingivitis and early periodontitis patients are suggested
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