1,720,954 research outputs found
Uso dell'enzima transglutaminsi come adiuvante tecnologico per la valorizzazione della componente proteica del siero refluo dalle industrie casearie
No full textSTUDI DI CARATTERIZZAZIONE DELLA rPrP OVINA MODIFICATA DALLA TRANSGLUTAMINASIMICROBICA
No full textLe proteine prioniche (PrP) sono molecole associate alle Encefalopatie Spongiformi Trasmissibili
(TSE), come la scrapie in pecora, la BSE nei bovini o il morbo di Creutzfeldt-Jacob nell’uomo (1). La
PrP è una glicoproteina espressa nell’encefalo dei mammiferi, dove è legata alla membrana
cellulare attraverso un’àncora di GPI (1). Alla base del ruolo svolto dalla PrP nelle “malattie da
prione” c’è un meccanismo molecolare che implica la conversione dell’isoforma cellulare PrPc in
PrPsc, una variante dotata di una infettività intrinseca. La PrPc ha una prevalente struttura ad α-
elica con una minima quota di β -sheet, mentre la PrPsc possiede essenzialmente una struttura di
tipo β (2) responsabile dell’aggregazione in oligomeri che possono evolvere nella formazione di
fibra amiloide (2,3).
In studi precedenti avevamo già dimostrato che la transglutaminasi microbica (mTG, enzima che
catalizza la formazione di legami isopeptidici intra e/o intermolecolari fra Q e K endo-proteiche) è
in grado di modificare la PrP ricombinante ovina (rPrP) introducendo dei legami intramolecolari
che alterano la mobilità elettroforetica della proteina. In particolare, la forma modificata presenta
una maggiore mobilità in SDS-PAGE rispetto a quella nativa. Nel presente lavoro l’attenzione è
stata rivolta alla caratterizzazione della rPrP modificata. Saggi enzimatici condotti in presenza
ammine primarie (che competono con le K della rPrP), quali idrossilammina, putrescina,
monodansilcadaverina e spermidina hanno confermato che la modificazione della rPrP è mTGdipendente.
Esperimenti di spettrometria di massa hanno permesso di rilevare la presenza di tre
legami isopeptidici intramolecolari, responsabili del cambiamento conformazionale della proteina
modificata che si riflette in un’aumentata mobilità elettroforetica. Il trattamento con la proteinasi
K (PK) ha evidenziato che, mentre la rPrP nativa è sensibile all’azione proteolitica della PK, la forma
modificata risulta resistente, comportamento tipico delle PrP ricche in struttura β (4).
Per valutare se i legami introdotti dalla mTG siano in grado di alterare la cinetica di formazione
della fibra amiloide, sono in corso esperimenti di aggregazione di rPrP modificata e non in cui la
formazione di fibra amiloide viene monitorata mediante l’uso della Tioflavina T
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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