1,720,962 research outputs found
Effetto diretto della 2-bromo-alfa-ergocriptina e della dopamina sulla contrattilità uterina in vitro
No full textSynthesis and characterization of a mini-library of new conjugated d(TGGGAG) oligonucleotides with potential anti-HIV activity.
No full textIn the search for ODNs endowed with relevant antiviral properties, Hotoda and coworkers investigated a series of G-quadruplex-forming ODNs, finally focusing on modified d(TGGGAG)
ODNs conjugated with aromatic residues at the 5-end. These were found to exhibit potent anti-HIV activity associated with low cytotoxicity when carrying at the 5′-end bulky aromatic residues.
Recently we described a general approach to obtain a mini library of new d(TGGGAG) ODNs, conjugated with different aromatic groups at the 5’-end through a phosphodiester bond. Several
modified sequences showed pronounced anti-HIV-1 activity and they showed high binding affinities for the HIV-1 envelope gp120 and gp41. In these structures the 5-end residues play a major role on the G-quadruplex stability, dramatically enhancing stability of the quadruplex complexes (Tm>20°C).
With the final goal to expand the repertoire of accessible end-modified G-rich ODNs, and to get a more complete picture of their structure-activity relationships, we describe herein the synthesis
and characterization of a mini-library of new d(5’TGGGAG3’) carrying hydrophobic groups at the 5’-end and 2-hydroxyethylphosphate group at the 3'-end, connected through phosphodiester and phosphoramidate bonds, respectively. In order to study the influence of the conjugation at the ends of the oligonucleotide chains on their ability to form quadruplex structures, a CD analysis was undertaken on the conjugated oligomers in comparison with the corresponding unmodified d(TGGGAG) oligomer
Effetto diretto della bromocriptina e della dopamina sulla contrattilità uterina in vitro
No full textHigher incidence of post-pill amenorrea in patients harbouring a prolactin (PRL) secretive tumour
No full textHarpin oligonucleotides forming G-quadruplexes: new aptamers with potential anti-HIV activity
No full textSeveral G-rich synthetic oligodeoxyribonucleotides (ODNs) have shown promising biological properties, ranging from anticancer to anti-HIV activities. G-quadruplex formation was found to be
a crucial prerequisite in determining these biological effects. Aptamers exhibiting anti-HIV activity represent an important class of potential therapeutics. Recently we described the
synthesis and characterization of new d(TGGGAG) ODNs, conjugated with different aromatic groups at the 5’-end through a phosphodiester bond. The modified sequences showed a parallel
stranded tetramolecular G-quadruplexes CD profile and a pronounced anti-HIV-1 activity. Herein, with the aim to use d(TGGGAG) as a lead sequence for a more effective anti-HIV
agent, we propose the fully automated synthesis of new ODNs containing two d(TGGGAG) sequences whose 3-ends are joint by an hexaethylenglycole loop. CD analysis was undertaken on
the 3’-3’ linked d(TGGGAG) hairpins in comparison with the corresponding unmodified oligomers. Besides, in order to study the influence of the conjugation at the ends of the harpin
chains on their ability to stabilize quadruplex structures and on their anti-HIV activity, different conjugated oligomers have been studied
Il laboratorio di analisi di impresa e l'elaborazione di un nuovo modello di rating per le piccole imprese rapporto finale di ricerca
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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