139 research outputs found
Shewanella pealeana sp. nov., a member of a microbial community associated with the accessory nidamental gland of the squid Loligo pealei.
No full textA new, mesophillic, facultatively anaerobic, psychrotolerant bacterium, strain ANG-SQ1T (T = type strain), was isolated from a microbial community colonizing the accessory nidamental gland of the squid Loligo pealei. It was selected from the community on the basis of its ability to reduce elemental sulfur. The cells are motile, Gram-negative rods (2.0-3.0 microns long, 0.4-0.6 micron wide). ANG-SQ1T grows optimally over the temperature range of 25-30 degrees C and a pH range of 6.5-7.5 degrees C in media containing 0.5 M NaCl. 16S rRNA sequence analysis revealed that this organism belongs to the gamma-3 subclass of the Proteobacteria. The closest relative of ANG-SQ1T is Shewanella gelidimarina, with a 16S rRNA sequence similarity of 97.0%. Growth occurs with glucose, lactate, acetate, pyruvate, glutamate, citrate, succinate, Casamino acids, yeast extract or peptone as sole energy source under aerobic conditions. The isolate grows anaerobically by the reduction of iron, manganese, nitrate, fumarate, trimethylamine-N-oxide, thiosulfate or elemental sulfur as terminal electron acceptor with lactate. Growth of ANG-SQ1T was enhanced by the addition of choline chloride to growth media lacking Casamino acids. The addition of leucine or valine also enhanced growth in minimal growth media supplemented with choline. The results of both phenotypic and genetic characterization indicate that ANG-SQ1T is a Shewanella species. Thus it is proposed that this new isolate be assigned to the genus Shewanella and that it should be named Shewanella pealeana sp. nov., in recognition of its association with L. peale
Risk factors for postoperative pain in need for intervention after general anaesthesia
No full textPatienten mit einem hohen Risiko für postoperative Schmerzen zu identifizieren
ermöglicht einen rechtzeitigen Therapiebeginn und eine adäquatere
Therapieplanung sowie die prophy-laktische Gabe eines Analgetikums. In
früheren Studien wird die Inzidenz an postoperativem Schmerz mit 20% – 80%
angegeben. In dieser retrospektiven Studie wurde an 1736 Patienten, die
Inzidenz von postoperativem therapiebedürftigem Schmerz (PPINI), definiert als
NRS >4 in Ruhe, direkt nach Erwachen im Aufwachraum untersucht. Es wurden
Patienten direkt nach Erwachen aus einer Allgemeinanästhesie im Aufwachraum
mittels einer „Numerischen Rating-Skala“ (NRS) nach ihres Schmerzbefindens
befragt. Primärer Zielparameter war postoperativer therapiebedürftiger Schmerz
(PPINI), definiert als NRS >4 in Ruhe. In unserer Studie hatten 28,5% der
Patienten postoperative therapiebedürftige Schmerzen. Als unabhängige
Prädiktoren für PPINI zeigten sich jüngeres Alter (OR = 1.300 [1.007–1.678], p
= 0.044), weibliches Geschlecht (OR = 1.494 [1.138–1.962], p = 0.004),
Adipositas (OR = 1.683 [1.226–2.310], p = 0.001), Gebrauch von N2O (OR = 1.621
[1.110–2.366], p = 0.012), längere Dauer der Operation (OR = 1.165
[1.050–1.292], p = 0.004), muskuloskelettale und intraabdominelle Eingriffe
(musculoskeletal OR = 2.026 [1.326–3.095], p = 0.001; intraabdominal OR =
1.869 [1.148–3.043], p = 0.012) sowie ASA-PS I-II (OR = 1.519 [1.131–2.039], P
= 0.005) als Risikofaktoren für PPINI. Die Identifizierung von Patienten mit
hohem Risiko für PPINI ist ein wichtiger Schritt, um das Schmerzmanagement im
postoperativen Verlauf zu optimieren.To identify patients at high risk for postoperative pain allows timely
initiation of therapy and a more adequate treatment planning as well as the
prophylactic administration of an analgesic. Previous studies describe a high
incidence of post-operative pain (20% to 80%). In this retrospective study, we
investigated 1736 Patients to identify the incidence of postoperative pain in
need of intervention ( PPINI ), defined as NRS > 4 at rest, examined
immediately after awakening in the recovery room. Patients were interviewed
directly after awakening from general anesthesia in the recovery by means of
the NRS (numeric rating scale). The primary endpoint was postoperative pain
requiring treatment ( PPINI ), defined as NRS > 4 at rest. In our study, 28.5
% of patients had postoperative pain requiring therapy (PPINI). On
multivariate analysis, younger age (OR = 1.300 [1.007–1.678], p = 0.044),
female gender (OR = 1.494 [1.138–1.962], p = 0.004), obesity (OR = 1.683
[1.226–2.310], p = 0.001), use of nitrous oxide (OR = 1.621 [1.110–2.366], p =
0.012), longer duration of surgery (OR = 1.165 [1.050–1.292], p = 0.004),
location of surgery (musculoskeletal OR = 2.026 [1.326–3.095], p = 0.001;
intraabdominal OR = 1.869 [1.148–3.043], p = 0.012), and ASA-PS I–II (OR =
1.519 [1.131–2.039], P = 0.005) were identified as independent risk factors
for PPINI. The identification of patients at high risk for PPINI is an
important step to optimize postoperative pain management
Relative tensor products and Koszul duality in monoidal oo-categories
No full textThis semi-expository work covers central aspects of the theory of relative
tensor products as developed in Higher Algebra, as well as their application to
Koszul duality for algebras in monoidal oo-categories. Part of our goal is to
expand on the rather condensed account of loc. cit. Along the way, we
generalize various aspects of the theory. For instance, given a monoidal
oo-category Cc, an oo-category Mm which is left-tensored over Cc, and an
algebra A in Cc, we construct an action of A-A-bimodules N in Cc on left
A-modules M in Mm by an "external relative tensor product" N \otimes_A M. (Up
until now, even the special ("internal") case Cc = Mm appears to have escaped
the literature.
As an application, we generalize the Koszul duality of loc. cit. to include
modules. Our straightforward approach requires that we at this point assume
certain compatibilities between tensor products and limits; these assumptions
have recently been shown to be unnecessary in work by Brantner, Campos and
Nuiten (arXiv:2104.03870).Comment: Some of the assumptions needed for our approach to Koszul duality for
left-modules in (LM-)monoidal oo-categories have been shown to be unnecessary
by Brantner, Campos and Nuiten in arXiv:2104.03870. We've reworked the
introduction to reflect this fact, and to highlight our foundational work on
external relative tensor product
Asotin County, Washington
No full textWalter Brooks and Elsie Garrison of Asotin, Washingtonhttps://digitalcommons.cwu.edu/edward_nolan/1012/thumbnail.jp
Lipoprotein X Causes Renal Disease in LCAT Deficiency.
No full textHuman familial lecithin:cholesterol acyltransferase (LCAT) deficiency (FLD) is characterized by low HDL, accumulation of an abnormal cholesterol-rich multilamellar particle called lipoprotein-X (LpX) in plasma, and renal disease. The aim of our study was to determine if LpX is nephrotoxic and to gain insight into the pathogenesis of FLD renal disease. We administered a synthetic LpX, nearly identical to endogenous LpX in its physical, chemical and biologic characteristics, to wild-type and Lcat-/- mice. Our in vitro and in vivo studies demonstrated an apoA-I and LCAT-dependent pathway for LpX conversion to HDL-like particles, which likely mediates normal plasma clearance of LpX. Plasma clearance of exogenous LpX was markedly delayed in Lcat-/- mice, which have low HDL, but only minimal amounts of endogenous LpX and do not spontaneously develop renal disease. Chronically administered exogenous LpX deposited in all renal glomerular cellular and matrical compartments of Lcat-/- mice, and induced proteinuria and nephrotoxic gene changes, as well as all of the hallmarks of FLD renal disease as assessed by histological, TEM, and SEM analyses. Extensive in vivo EM studies revealed LpX uptake by macropinocytosis into mouse glomerular endothelial cells, podocytes, and mesangial cells and delivery to lysosomes where it was degraded. Endocytosed LpX appeared to be degraded by both human podocyte and mesangial cell lysosomal PLA2 and induced podocyte secretion of pro-inflammatory IL-6 in vitro and renal Cxl10 expression in Lcat-/- mice. In conclusion, LpX is a nephrotoxic particle that in the absence of Lcat induces all of the histological and functional hallmarks of FLD and hence may serve as a biomarker for monitoring recombinant LCAT therapy. In addition, our studies suggest that LpX-induced loss of endothelial barrier function and release of cytokines by renal glomerular cells likely plays a role in the initiation and progression of FLD nephrosis
Formal Integration of Derived Foliations
No full textFrobenius' theorem in differential geometry asserts that every involutive subbundle of the tangent bundle of a manifold M integrates to a decomposition of M into smooth leaves. We prove an infinitesimal analogue of this result for locally coherent qcqs schemes X over coherent rings. More precisely, we integrate partition Lie algebroids on X to formal moduli stacks X → S, where S is the formal leaf space and the fibres of X → S are the formal leaves. We deduce that deformations of X-families of algebro-geometric objects are controlled by partition Lie algebroids on X. Combining our integration equivalence with a result of Fu, we deduce that Toën–Vezzosi's infinitesimal derived foliations (under suitable finiteness hypotheses) are formally integrable
Formal Integration of Derived Foliations
No full textFrobenius' theorem in differential geometry asserts that every involutive subbundle of the tangent bundle of a manifold M integrates to a decomposition of M into smooth leaves. We prove an infinitesimal analogue of this result for locally coherent qcqs schemes X over coherent rings. More precisely, we integrate partition Lie algebroids on X to formal moduli stacks X → S, where S is the formal leaf space and the fibres of X → S are the formal leaves. We deduce that deformations of X-families of algebro-geometric objects are controlled by partition Lie algebroids on X. Combining our integration equivalence with a result of Fu, we deduce that Toën–Vezzosi's infinitesimal derived foliations (under suitable finiteness hypotheses) are formally integrable
Formal Integration of Derived Foliations
No full textFrobenius' theorem in differential geometry asserts that every involutive subbundle of the tangent bundle of a manifold M integrates to a decomposition of M into smooth leaves. We prove an infinitesimal analogue of this result for locally coherent qcqs schemes X over coherent rings. More precisely, we integrate partition Lie algebroids on X to formal moduli stacks X → S, where S is the formal leaf space and the fibres of X → S are the formal leaves. We deduce that deformations of X-families of algebro-geometric objects are controlled by partition Lie algebroids on X. Combining our integration equivalence with a result of Fu, we deduce that Toën–Vezzosi's infinitesimal derived foliations (under suitable finiteness hypotheses) are formally integrable
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