57 research outputs found
Evaluation of intra-cultivar variability in Olea europaea L. cv. Leccino using morphological, biochemical and molecular markers
No full textThe phenotypic and genotypic variability of olive tree cv. Leccino (Olea europaea L.), an ancient cultivar from North-Central Italy, was investigated using 44 quantitative and qualitative agro-morphological traits, SDS-PAGE of seed proteins (storage proteins and tegument proteins) and molecular markers such as RAPDs (30 primers) and SSRs (8 primers). Fifteen accessions of Olea europaea catalogued as "Leccino" and one certified tree of "Leccino" (LESt) were examined. The plants were clonally propagated and the measuring of all morphological characteristics was conducted over a period of seven years. The ANOVA analysis showed that all accessions were homogenous for many traits while very few variations were recorded for length of one year old shoots, leaf width, leaf length/leaf width ratio, fruit length, fruit fresh weight, fruit length/fruit width ratio, pit length and pit fresh weight. The PCA analysis and similarity coefficients confirmed a low level of variability of Leccino cultivar. SDS-PAGE analysis of seed proteins showed monomorphic patterns of storage proteins. Protein subunits of teguments revealed a generally high level of similarity as evidenced by Nei-Li coefficient. SSRs and RAPDs markers showed molecular monomorphism among Leccino accessions. The results of agro-morphological, biochemical and molecular nature, taken as a whole, seem to indicate a weakly differentiated/homogeneity of the accessions tested belonging to the Leccino cultivar. The limited morphological and genetic variation could support the assumptions of a monophyletic origin of Leccino cultivar with a genetically restricted base
[Refractory heart failure. Positive inotropes and beta blockers: alternative or complementary treatments?].
No full textDiffuse-light absorption spectroscopy and chemometrics for discrimination and quantification of extra virgin olive oil adulterants
No full textA selection of top-quality extra virgin olive oils with excellent nutraceutic properties was artificially adulterated by means of lower-grade olive oils commonly used in commercial counterfeits. The oil sample set was analyzed by means of diffuse-light absorption spectroscopy performed in the wide 400–1700 nm spectral range. A setup based on optical fiber technology was experimented, which made use of an optical fiber supercontinuum source. The spectroscopic library was processed by means of a Partial Least Squares regression for quantifying the adulterant concentration, followed by a Principal Component Analysis and Linear Discriminant Analysis for identifying the type of adulterant
Role of aging, chronic renal failure and dialysis in the calcification of mitral anulus.
No full textVisible and near-infrared absorption spectroscopy by an integrating sphere and optical fibers for quantifying and discriminating the adulteration of extra virgin olive oil from Tuscany
No full textBecause of its high price, extra virgin olive oil is frequently targeted for adulteration with lower quality oils. This paper presents an innovative optical technique capable of quantifying and discriminating the adulteration of extra virgin olive oil caused by lower-grade olive oils. An original set-up for diffuse-light absorption spectroscopy in the wide 400–1,700 nm spectral range was experimented. It made use of an integrating sphere containing the oil sample and of optical fibers for illumination and detection; it provided intrinsically scattering-free absorption spectrosco- py measurements. This set-up was used to collect spectro-scopic fingerprints of authentic extra virgin olive oils from the Italian Tuscany region, adulterated by different concentrations of olive-pomace oil, refined olive oil, deodorized olive oil, and refined olive-pomace oil. Then, a straightforward multivariate processing of spectroscopic data based on principal component analysis and linear discriminant analysis was applied which was successfully capable of predicting the fraction of adulterant in the mixture, and of discriminating its type. The results achieved by means of optical spectroscopy were compared with the analysis of fatty acids, which was carried out by standard gas chromatography
Visible and near.infrared soectral signatures for adulteration of extra virgin olive oil
No full textA smart cap for olive oil rancidity detection using optochemical sensors
No full textThe design and experimental setup of a smart cap are presented. It is capable of sniffing the vapors of extra virgin olive oil, thus alerting the consumer or the retailer of any rancid flavor. The cap is made of an array of metalloporphyrin-based optochemical sensors, the colors of which are modulated by the concentration of aldehydes, the main responsible for rancid off-flavors. A micro-optic device, implemented to simulate a cap prototype, is presented. The spectral response of the chromophore-array is processed by means of multivariate data analysis so as to achieve an artificial olfactory perception of oil aroma and, consequently, an indication of oil ageing and rancidity. In practice, the cap prototype proved to be a device for non-destructive testing of bottled oil quality
Comparative analysis of quality parameters of Italian extra virgin olive oils according to their region of origin
No full textItalian extra virgin olive oils from four regions covering different latitudes of the country were considered. They were
analyzed by means of absorption spectroscopy in the wide 200-2800 nm spectral range, and multivariate data processing
was applied. These spectra were virtually a signature identification from which to extract information on the region of
origin and on the most important quality indicators. A classification map was created which was able to group the 80 oils
on the basis of their region of origin. Furthermore, a model for the prediction of quality parameters such as oleic acidity,
peroxide number, K232, K270 and Delta K, was developed
Comparison of long-term outcome in anthracycline-related versus idiopathic dilated cardiomyopathy: a single centre experience
No full textAims Cardiac dysfunction is a severe complication of anthracycline-containing anticancer therapy. The outcome of anthracycline-induced cardiomyopathy (AICM) compared with other non-ischaemic causes of heart failure (HF), such as idiopathic dilated cardiomyopathy (IDCM), is unresolved. The aim of this study was to compare the survival of AICM patients with an IDCM cohort followed at our centre from 1990 to 2016. Methods and results We included 67 patients (67% female, 50 +/- 15 years) with AICM, defined as onset of otherwise unexplained left ventricular ejection fraction (LVEF) <= 50% following anthracycline therapy, and 488 IDCM patients (28% female, 55 +/- 12 years). Patients were followed with constantly optimized HF therapy, for 7.6 +/- 5.5 and 8.1 +/- 5.5 years, respectively. In both cohorts, 25% of patients reached the combined endpoint of death/heart transplantation. Overall survival rates at 5 and 10 years were similar (AICM: 86% and 61%, IDCM: 88% and 75%; P = 0.61), and so was cardiovascular survival (AICM: 91% and 76%, IDCM: 91% and 80%; P = 0.373), also after 1:1 propensity matching (P = 0.27) and adjusting for age, LVEF and left ventricular size. A trend toward higher all-cause mortality was present in AICM patients [hazard ratio (HR) 1.67, 95% confidence interval (CI) 0.95-2.92, P = 0.076]. No differences were observed between AICM and IDCM with regard to pharmacological HF therapy, but AICM patients were less likely to receive devices (13% vs. 41.8% in IDCM, P < 0.001). Conclusion Cardiovascular mortality in patients with AICM did not differ from that of a matched IDCM cohort, despite cancer-related morbidity and less prevalent use of devices. These data suggest that patients with AICM should be treated with appropriate guideline-directed medical therapies similar to other non-ischaemic dilated cardiomyopathies
Eficiecia de una nueva escala para la caracterizaciòn del color de aceites de oliva vìrgenes de procediencia italiana
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