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Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
No full textMehmood, Nasir, Yuan, Yuan, Ali, Mohammed, Ali, Muhammad, Iftikhar, Junaid, Cheng, Chunzhen, Lyu, Meiling, Wu, Binghua (2021): Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis. Phytochemistry (112590) 181: 1-12, DOI: 10.1016/j.phytochem.2020.112590, URL: http://dx.doi.org/10.1016/j.phytochem.2020.11259
Fig. 4 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
No full textFig. 4. Schematic illustration of general terpenoid biosynthesis pathways showing identified unigenes from F. nilgerrensis leaf transcriptome data. The interconvertible precursors IPP and DMAPP, two phosphorylated C5 unites, are produced by the MVA and MEP pathways which are exchangeable from both compartmentations. The chloroplast is a major site for synthesis of hemiterpene (C5), monoterpenoids (C10), diterpenoids (C20) carotenoids (C40) and chlorophyll, while the cytosol and other organelle are responsible for synthesis of monoterpenoids (C10), sesquiterpene (C15) and triterpene (C30). But that is not strictly conclusive. Arrow with lines indicate reactions catalyzed by enzymes and the encoding genes, with unigenes identified in this experiment boxed. The color highlights are for better visualization. Abbreviations: AACT, acetoacetyl-CoA thiolase; CMK, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; DXR, 1-deoxy-D-xylulose 5-phosphate reductase; DXS, 1-deoxy-D-xylulose 5-phosphate synthase; HDR, (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase; HDS, (E)-4-hydroxy-3- methyl-but-2-enyl diphosphate synthase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; IDI, isopentenyl diphosphate isomerase; MCT, MEP cytidyltransferase; MDC, mevalonate-5-diphosphate decarboxylase; MDS, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MVK, mevalonate kinase. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)Published as part of Mehmood, Nasir, Yuan, Yuan, Ali, Mohammed, Ali, Muhammad, Iftikhar, Junaid, Cheng, Chunzhen, Lyu, Meiling & Wu, Binghua, 2021, Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis, pp. 1-12 in Phytochemistry (112590) 181 on page 7, DOI: 10.1016/j.phytochem.2020.112590, http://zenodo.org/record/829067
Fig. 7 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
No full textFig. 7. Phylogenetic relationship of the five FnTPS candidates with other known terpene synthases and a sequence alignment showing the conserved protein motifs. (A) A maximum-likelihood tree of the TPS proteins depicting the TPS-a, TPS-b, TPS-d, TPS-e/f, and TPS-g clades, with bootstrap values greater than 50% shown for the branching. The scale bar corresponds to 6% amino acid substitution. The five F. nilgerrensis proteins are in red. Selected proteins with available three-dimensional structural data are shown in bold using their PDB code followed by the abbreviated species name. The known enzymatic products are in light blue followed with the UniProt Accession numbers of the proteins. (B) Alignment of the five candidates FnTPSs with α-farnesene synthase from apple (Malus domestica) and α-bergamotene synthase from Lavender (Lavandula Angustifolia). The DxDD motif of typical class II terpene synthases in FnTPS6 is boxed and the highly conserved class I DDxxD as well as the lesser conserved RRx8W and NSE/DTE motifs are indicated. The color regime of amino acids is set in Bioedit version 7.2.6. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)Published as part of Mehmood, Nasir, Yuan, Yuan, Ali, Mohammed, Ali, Muhammad, Iftikhar, Junaid, Cheng, Chunzhen, Lyu, Meiling & Wu, Binghua, 2021, Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis, pp. 1-12 in Phytochemistry (112590) 181 on page 10, DOI: 10.1016/j.phytochem.2020.112590, http://zenodo.org/record/829067
Fragaria nilgerrensis leaves
No full text4.2. Isolation of phytochemical and volatile compounds from F. nilgerrensis leaves At different hours (0, 3, 6, 12, 18, 24, 48 and 72 h) after infected with C. gloeosporioides, leaves were removed and collected from the plants, frozen immediately in liquid nitrogen and then stored at 80 ◦ C for future use. Each cryopreserved sample was homogenized in liquid nitrogen by using pestle and mortar. One gram of the powdered samples was mixed with 2 ml saturated NaCl in a 20 ml-head-space vial, in which an internal standard (10 μl of 8.08 mg /L 2,6-dimethyl-4-heptanone (cat. Nr. W353701 from Sigma–Aldrich, Shanghai, China) was added. Before subjecting the mixtures for SPME extraction, each vial was heated at 60 ◦ C for 10 min. Subsequently, the headspace of the sample vial was exposed to a 65 μm divinylbenzene/carboxen/polydimethylsilioxan fiber (Supelco, Bellefonte, PA, USA) for 20 min at 60 ◦ C to complete the extraction. Relative abundance of the compounds was quantified by peak area determination and normalized to the mass of the internal standard. We pooled samples from three plants for one biological replication and the experiment was performed twice which gave similar results. Data are shown as mean without significant test in the Results section.Published as part of Mehmood, Nasir, Yuan, Yuan, Ali, Mohammed, Ali, Muhammad, Iftikhar, Junaid, Cheng, Chunzhen, Lyu, Meiling & Wu, Binghua, 2021, Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis, pp. 1-12 in Phytochemistry (112590) (112590) 181 on page 7, DOI: 10.1016/j.phytochem.2020.112590, http://zenodo.org/record/829067
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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