1,720,953 research outputs found
Proteomics analyses of Fli-1 transgenic zebrafish
No full text血管內皮是一層緊密排列在各種血管內壁的表面細胞層，在血管空腔的循環血流與血管壁中形成一個交界面，內皮細胞在血管發育、凝血、免疫系統甚至在動脈硬化與癌症中都扮演著重要的角色。血管的正常發育明顯是胚胎發育的關鍵，然而在成人中血管發育的異常則容易導致疾病的產生，例如: 腫瘤的生長。另一方面，在人類疾病的診斷上生物標誌是一個被普遍運用的工具。Fli-1: EGFP基因轉殖斑馬魚在發育過程中，fli-1啟動子可以驅動EGFP表現在血管組織。在此研究中，我們希望能藉由Fli-1: EGFP基因轉殖斑馬魚胚胎來探索新的血管內皮細胞生物標誌。首先，我們個別收集並分離 4 hpf 與120 hpf 的Fli-1: EGFP基因轉殖斑馬魚胚胎，接著利用流式細胞分選儀 (FACSAria) 分選出帶有綠色螢光的血管內皮細胞。在進行分選前，先將受精後4小時Fli-1: EGFP的胚胎細胞與餵養細胞進行短暫共同培養來增加細胞數量。接著利用SDS-PAGE分離細胞萃取液後將蛋白質片段切下並進行膠內水解後再以 LC/MS/MS 分析，最後利用 MSCOT 資料庫(http://www.matrixscience.com ) 分析所得 MS 數據。血管內皮細胞在兩個不同發育階段中蛋白質型態呈現著不同的分布，比較實驗的數據，我們可以發現一些發育階段專一性蛋白的表現與可能的血管內皮細胞生物標誌，Thrombospondin-4A、4B。更進一步，我們製備針對斑馬魚Thrombospondin-4A、4B的抗體並透過免疫染色分析發現Thrombospondin-4A與已知的血管內皮細胞生物標誌 (VEGFR1、VEGFR2 與 Tie-2) 表達在相同的位置。Thrombospondin-4A具有可以在人類疾病管理上作為臨床診斷與預後生物標誌的潛能。The vascular endothelium is a thin layer of cells that lines the interior surface of blood vessels, forming an interface between circulating blood in the lumen and the rest of the vessel wall. Vascular endothelial cells play an important role in vascular development, blood coagulation, immune system and even in atherosclerosis and cancer. It has been reported that normal blood vessel formation is critical for embryonic development, whereas the aberrant blood vessel formation in the adult contributes to pathologies such as tumor growth. On the other hand, biomarker is a prevalent tool for early diagnosis of human diseases. Zebrafish fli-1 promoter is able to drive expression of EGFP in all blood vessels throughout embryogenesis. In this study, we intend to identify some novel biomarker of vascular endothelial cells by using Fli-1: EGFP transgenic zebrafish embryos. First, we collected and isolated cells of Fli-1: EGFP transgenic zebrafish embryo at 4hpf and 120hpf and then the EGFP positive vascular endothelial cells were sorted by Flow cytometry (FACSAria). The cells from 4hpf Fli-1 embryos were shortly co-cultured with feeder layer to increase the cell number (for expanding cell population) before sorting process. After resolving the total lysate from the sorted cells by SDS-PAGE, the separated proteins were subjected to trypsin in gel digestion followed by LC/MS/MS analysis. The data of proteomics analysis present the different protein patterns in these two stages. According to these data, we could identify some developmental stage-specific proteins and potential vascular endothelial cell biomarkers, Thrombospondin-4A and 4B. Furthermore, we clone the zebrafish TSP4A and TSP4B for the His-TSP4A and His-TSP4B fusion proteins and generate the antibodies against these antigens. By using immuno-staining, we could find out zebrafish Thrombospondin-4A respectively co-localize with VEGFR1、VEGFR2 and Tie-2. Thrombospondin-4A may be a biomarker for clinical diagnosis or prognosis in human disease management
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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