54 research outputs found
Comparison of proliferation and rapid cytokine induction assays for flow cytometric T‐cell epitope mapping
No full textQuantum optical processes: from basics to applications
No full textThis book is a self-contained guide to the world of quantum optical processes which addresses different aspects relevant in quantum optics and quantum information. The basic descriptions, measurement techniques, possible sources, nonclassical features, practical implications and applications of the quantization of light and its interaction with matter, are explored. The observed quantum properties such as coherent superposition, entanglement, nonlocality, decoherence and no-cloning, are discussed. The quantum optical processes such as continuous variable entanglement swapping, teleportation and telecloning from which follow the practical aspects such as quantum gate operations, cryptography and error correction are considered. In turn, the advantages and inherent challenges including the foresight in implementing continuous variable quantum communication and computation protocols are highlighted. The author gives a concise background with corresponding applications, the necessary mathematical derivation, simplified examples, illustrations and demonstrations, and the relative interpretations and outlooks. This book is intended to serve a multi-disciplinary readership, namely the atomic physics and quantum optics communities who seek to extend their research to applications, especially, to the field of quantum information processing as well as the theoretical quantum information community who builds up research on physically realizable systems such as optical setups and various atomic schemes. The content of this book also attracts other communities such as photonics who seeks to link research with continuous variable quantum information processing
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Contexts of Care: Supporting Immigrant-Origin Students and Families in a New Destination
No full textThis dissertation examines how educators, families, and youth collectively build caring contexts of reception in a growing, diversifying school district in the United States. Drawing on over 800 hours of embodied ethnographic research conducted during the 2023–2024 school year, the study explores the daily experiences of immigrant educators, students, and families. Merging critical qualitative ethnographic research with applied experience, the author worked as an Amharic- and Spanish-speaking family liaison across the district and as a college access mentor for immigrant-origin students in a college preparation program for immigrant-origin youth.
First, the study demonstrates that immigrant educators develop creative strategies to meet both individual and collective needs within institutional constraints. These educators actively work to interrupt systemic harm while navigating educational structures that often perpetuate inequities for immigrant-origin students. Second, the research illuminates how multiple actors—including students, families, and educators—interact within layered institutional contexts. An intersectional analysis reveals that these interactions are significantly shaped by factors beyond immigration status alone, including income, housing status, and national origin. Third, the findings show that caring educational practices emerge in complex and sometimes contradictory ways. While symbolic gestures of inclusion exist, the most effective practices address material needs and structural barriers facing immigrant communities. Fourth, the data indicates that student outcomes are closely tied to the availability of multilingual staff and resources that reflect the diverse backgrounds of the student population. Schools with more comprehensive linguistic support systems demonstrated more positive integration experiences. Finally, the research documents how immigrant youth themselves develop resilience strategies and peer support networks that function as protective mechanisms against exclusion and discrimination. These student-led initiatives often operate alongside formal institutional supports but fill critical gaps in the educational context of reception.
The dissertation concludes with policy recommendations that address structural barriers facing immigrant communities, including expanding higher education access for students with liminal immigration statuses, implementing staffing practices that reflect student diversity, providing attendance stipends for low-income students, and developing welcoming school cultures that address material needs beyond symbolic gestures. These recommendations are grounded in transformative care ethics and target multiple dimensions of contexts of reception, recognizing that meaningful immigrant integration requires coordinated interventions across educational and social domains.Educatio
Inhibition of Akt activity and calcium channel function coordinately drive cell-cell fusion in the BeWO choriocarcinoma placental cell line
No full textTo establish a simple and quantitative live cell fusion assay for placental syncytialization, we generated stable GFP and dsRed expressing fusogenic BeWo cell lines. Fluorescent Activated Cell Sorting was shown to provide a quantitative determination of forskolin (cAMP-mediated) fusion in a time and concentration dependent manner consistent with the increased secretion of beta human chorionic gonadotrophin (β-HCG) and appearance of multi-nucleated cells. Analyses of the fusion process demonstrated that in addition to increased cAMP levels, simultaneous reduction of intracellular calcium and inhibition of Type 1 phosphatidylinositol 3 kinase (PI3K)/Akt signaling also resulted in cell fusion. Although individual blockade of calcium channel function or PI3K/Akt signaling was without effect, the combination with forskolin resulted in a potentiation of cell fusion. These data demonstrate syncytialization is a complex process that depends upon the regulation of distinct signaling inputs that function in concert with each other
Induction of fetal/embryonic globin gene expression depends on intact cell signaling in definitive erythroid cells☆
No full textBensal HP Treatment for Burn and Excision Wounds: An In-Vivo Assessment of Wound Healing Efficacy and Immunological Impact.
No full textCell type-specific chromatin accessibility analysis in the mouse and human brain
No full textThe Assay for Transposase Accessible Chromatin by sequencing (ATAC-seq) is becoming popular in the neuroscience field where chromatin regulation is thought to be involved in neurodevelopment, activity-dependent gene regulation, hormonal and environmental responses, and pathophysiology of neuropsychiatric disorders. The advantages of using ATAC-seq include a small amount of material needed, fast protocol, and the ability to capture a range of gene regulatory elements with a single assay. With increasing interest in chromatin research, it is an imperative to have feasible, reliable assays that are compatible with a range of neuroscience study designs. Here we tested three protocols for neuronal chromatin accessibility analysis, including a varying brain tissue freezing method followed by fluorescence-activated nuclei sorting (FANS) and ATAC-seq. Our study shows that the cryopreservation method impacts the number of open chromatin regions identified from frozen brain tissue using ATAC-seq. However, we show that all protocols generate consistent and robust data and enable the identification of functional regulatory elements in neuronal cells. Our study implies that the broad biological interpretation of chromatin accessibility data is not significantly affected by the freezing condition. We also reveal additional challenges of doing chromatin analysis on post-mortem human brain tissue. Overall, ATAC-seq coupled with FANS is a powerful method to capture cell-type-specific chromatin accessibility information in mouse and human brain. Our study provides alternative brain preservation methods that generate high-quality ATAC-seq data while fitting in different study designs, and further encourages the use of this method to uncover the role of epigenetic (dys)regulation in the brain
Macrophage autophagy limits acute toxic liver injury in mice through down regulation of interleukin-1β
No full textFunctional variant analyses (FVAs) predict pathogenicity in the BRCA1 DNA double-strand break repair pathway
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