4,236 research outputs found
Suppression of the Pth(Ts) phenotype mediated by the overproduction of tRNA maintains moderate levels of the Pth(Ts) protein
No full text<p><b>Copyright information:</b></p><p>Taken from "Excess of charged tRNA maintains low levels of peptidyl-tRNA hydrolase in (Ts) mutants at a non-permissive temperature"</p><p>Nucleic Acids Research 2006;34(5):1564-1570.</p><p>Published online 15 Mar 2006</p><p>PMCID:PMC1408313.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Depicts the cellular growth of the (Ts) mutant strain AA7852 separately transformed with pVH124 (ΔU, ΔV), pVH125 (U, ΔV) or pVH119 (U, V) incubated at different temperatures. Isolated colonies of the independent transformants were streaked onto LB-Ap plates and incubated overnight at the indicated temperatures. () Presents the immunodetection of Pth(Ts) in the (Ts) mutant strain AA7852 separately transformed with pVH124, pVH125, pVH119, ptRNACCA (X, R, T, M) or pTH2 (W) and grown at 32°C prior to transfer at time = 0 min at 41 or 43°C. The concentration of Pth(Ts) protein was estimated by immunoblot analysis. The left lane shows purified wild-type Pth protein, which migrates slightly faster in SDS–PAGE than the Pth(Ts) variant (arrowed) ()
Emergence of oblique TS mode due to longitudinal wall oscillation in 2D channel flow
No full textStabilizing effects of wall oscillation on two dimensional (2D) Tollmein-Schlichting (TS) waves and oblique TS waves developing in 2D channel flow are numerically investigated using the Floquet method. It was shown from the previous study[1] that the wall oscillation mostly has stabilizing effect and then TS mode is occasionally more stable than the oblique mode. In the present study the characteristics of the stability are investigated in detail. Then it is cleared that the exchange of the most unstable mode form the 2D TS to the oblique TS can occur at a certain value of Reynolds number
Modelling and Design of Photovoltaic Fed Re-lift Luo Converter for Air Conditioner
No full textThe design of high gain converter with photovoltaic (PV) module is carried out in this paper for air conditioning application. The solar based power system gaining large importance due to the renewable nature and increased life time. To understand power and voltage characteristic of PV module, the five parameter single diode model is evolved and analyzed. Due to the varying power characteristic of PV panel to the temperature and irradiance change, perturb and observe maximum power point tracking algorithm is imposed to harvest the maximal potential PV power. Air conditioner require high currents so solar modules are connected in parallel instead of series configuration. Since the output voltage of PV module is low, it requires a DC to DC power electronic converter with large voltage gain to boost the output voltage. Various converters such as conventional boost, buck/boost and Luo converters to provide high voltage gain are analyzed in this work and Luo converter is found to give small ripple in current and voltage as compared to the other two converters for the same voltage gain. Further, in Luo converter, voltage gain can be improved by lifting technique in which self-lift and re-lift techniques are employed. The Re-lift Luo converter has high voltage gains twice that of self-lift Luo and reduced ripple for solar power application. For air conditioning application AC output is obtained using a full bridge single phase voltage source inverter
A TS uszályhíd kifejlesztése és alkalmazása
No full textThe essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers.The essay focuses on the history of floating bridges and their special situation in Hungary. It produces the main elements and building procedures of the TS barge bridge. The author writes an appreciation of the TS barge bridge designers
The new CEN/TS 19100: Design of glass structures
No full textSince the beginning of 2021, CEN/TS 19100 Design of Glass Structures has been available in its first three parts. The fourth part is expected soon. This Technical Specification of the European standards organisation CEN is as a pre-standard of a corresponding future Eurocode. These documents constitute the first ever comprehensive design code for the entire structural glass engineering field on the European market for the first time. In addition to a clear outline, the Technical Specification has been drafted to be compatible with EN 1990 “Basis of Design” and to address glass-specific design matters, particularly related to robustness and redundancy. Although the standard still has the status of a CEN/TS, thereby allowing the European nations the option of whether to introduce it, either in full or in parts, it already contains national openings through which the European countries can adapt the design results to their own safety level by National Determined Parameters (NDPs). Such an approach already anticipates the future Eurocode, which is expected to be published as EN 19100—Design of Glass Structures. This article provides some context on the history and concept behind the new documents and gives an overview of the design rules and the corresponding technical background of the different parts of CEN/TS 19100.Applied MechanicsArchitectural Technolog
Investigation of grinding performance in ultrasonic vibration assisted grinding of Ti-6Al4V alloy using minimum quantity lubrication
No full textMinimum quantity lubrication (MQL) is an efficient cooling and lubrication technique usually used these days in grinding operation. It isfound to be advantageousfor improving the grinding performance in terms of reduced grinding forces, surface integrity and production cost since it offers better cooling, lubrication, and lower coolant consumption. Ultrasonic vibration assisted grinding (UAG) has also shown the improvement in the grinding performance owing to the change in the nature of cutting process in UAG. In this study, the grinding performance of UAG combined with MQL using soluble oil on Ti-6Al-4V alloy is studied through surface grinding experiments. The results show significant improvement in surface finish and reduction in grinding forces are achieved in UAG with MQL grinding process as compared with conventional dry and ultrasonic vibration assisted dry grinding
Development of EMT/TS Co-simulation Using PowerFactory and PSS/E
No full textAs the scale and complexity of power systems increase, simulating them in efficient and accurate ways continues tobe a challenge in power systems engineering. Electromagnetic Transient (EMT) and Transient Stability (TS) simulation are the two main dynamic power system simulation methods. To simulate large and complex power systems in sufficient detail without sacrificing execution time, one of the idea is to perform a co-simulation that couples EMT and TS simulator. Although several attempts have been made to couple both simulator, only on rare occasions do these hybrid EMT-TS simulators couple two or more industry-adopted simulation tools.The objective of this thesis is to to develop and study the benefits and limitations of the Electromagnetic Transient – Transient Stability co-simulation based on PowerFactory and PSS/E, both of which are among the most extensively used simulation tools in industry and academia alike. With regards to the objective, the EMT-TS co-simulation using PowerFactory and PSS/E has been developed. Then, several tests are performed to evaluate the function of each composing part of the EMT-TS co-simulation, and to test the integration between all its component. Next, The developed co-simulation is applied to study cases and the results are compared to a monolithic EMT simulation to evaluate its accuracy and execution time. Furthermore, the effect of TS and EMT time step to the accuracy and execution time of EMT-TS co-simulation have also been investigated.The study case results show that the developed EMT-TS co-simulation has not been beneficial yet in terms of accuracy and execution time. Although the active power result shows a similar tendency with the monolithic EMT result, the difference between both are visible. The difference between both are more prominent in the reactive power result. The total execution time of the developed co-simulation in the study cases are in the range of 23-24 minutes, significantly larger than the total execution time obtained from the monolithic EMT simulation which is around of 12s. Also, it is found that reducing the TS time step from 0.02 s to 0.01 s slightly increases the total simulation time from 23 to 26 minutes. However, it does not contribute a significant improvement on the accuracy of the developed EMT-TS co-simulation. The result obtained from reducing the EMT time step to is the same with the result obtained using EMT time step. Moreover, the reduction of the EMT time step significantly increases the total simulation time from 23 to 42 minutes. The developed co-simulation still has a lot of room for improvement and further developments in this topic might increase its performance.Electrical Engineering | Sustainable Energy Technolog
A reliable amplification technique with single-sided specificity for the isolation of 5' gene regulating regions.
No full textA simple and efficient method is described for the isolation of extension fragments of known DNA sequences by polymerase chain reaction (PCR) using a single specific primer. With this method, size-selected genomic DNA fragments are ligated to a plasmid Vector (pGEM-4Z) which contains sequencing primers and the population of chimeric plasmids is used for transforming Escherichia coli. DNA is extracted from an aliquot of the resulting mini-library and PCR performed using a sequence-specific primer and either of the standard sequencing primers of the plasmid vector. This method appears to be more Versatile than inverse PCR (IPCR), since: ii) the DNA sequence needed as the specific primer can be as short as about 20 nucleotides (nt); (ii) the DNA templates to be used in PCR are available in high amount, thus facilitating all manipulations; and (iii) if relinearization of the DNA by restriction enzyme digestion is desired before the PCR reaction, many restriction sites can be chosen from the vector polylinker. Using this method, we have isolated the genomic 5' region of the carrot bifunctional dihydrofolate reductase-thymidylate synthase-encoding gene dhfr-ts using a 21-nt sequence of the 5' region of the dhfr-ts cDNA clone as the specific primer
Molecular cloning and analysis of a cDNA coding for the bifunctional dihydrofolate reductase-thymidylate synthase of Daucus carota.
No full textMolecular cloning of dihydrofolate reductase-thymidylate synthase (DHFR-TS) of Daucus carota was
achieved by immunoscreening of a cDNA library obtaining a 2 kbp clone which contains an open reading
frame of 1528 bp. Comparison of the deduced amino acid sequence with those from other sources revealed the presence of motifs typical of DHFR and TS thus confirming the bifunctional nature of the carrot protein. As in other organisms, a higher degree of conservation was observed in the TS domain. Analysis of the dhfr-ts gene content in carrot revealed the presence of several copies per diploid genome
Analysis of the structure of the 5' end of the gene coding for carrot dihydrofolate reductase-thymidylate synthase.
No full textCarrot cells contain two transcript types for the dhfr-ts gene and evidence was obtained for a plastidial localization of the product of the longer transcripts. This indication contrasted with previous circumstantial evidence which suggested a cytosolic Iodization. The conflicting evidence was found to bt due to the presence of a large intron in the region corresponding to the 5' end of the mRNA. In this paper we report the structure of the genomic region corresponding to the 5' end of the carrot DHFR-TS-encoding gene and the cloning by inverse polymerase chain reaction of the promoter region which has been partially sequenced showing the presence of putative TATA boxes
and AT-rich regions. It is also observed that the two dhfr-ts gene transcripts contain TC repeats and a TC-rich stretch, respectively, whose possible role in the regulation of gene expression is discussed
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