1,721,480 research outputs found
The role of HMGCR in colorectal cancer and the study of lncRNA in lung cancer TKI-resistance
No full textHMGCR (3-Hydroxy-3-methyl-glutaryl-CoA reductase, HMGCR)是合成膽固醇的速率決定酵素，將 HMG-CoA轉換成mevalonate，因此膽固醇生合成路徑也稱為mevalonate pathway；活化mevalonate pathway會促進GTPase farnesylation，加速細胞之生長與癌化；臨床上，服用Statins (HMGCR 抑制劑)可減少血中膽固醇濃度，降低心血管與腦血管疾病之發生率。大腸直腸癌(Colorectal Cancer, CRC)為世界發生率第三高之癌症，其患者腫瘤組織之HMGCR活性較正常組織高，服用statins可降低罹患大腸直腸癌之風險，我們使用HMGCR表現較多的HCT116以及HMGCR表現較低的SW480來探討HMGCR在大腸直腸癌的角色。 使用shRNA knockdown或CRISPR knockout HCT116之HMGCR，會增加apoptotic marker (Cleaved caspase-3)之表現，減少pro-survival (Mcl-1)之表現；Overexpress HMGCR至SW480則增加Mcl-1之表現，與發炎相關的COX-2以及引發DNA de novo methylation的DNMT3A之表現亦增加，推論HMGCR是CRC致癌因子之一。 我們以RNA sequencing分析兩組TKI-resistant 細胞，HCC827 vs HCC827/IR和H1975 vs H1975/AR，發現參與調控cell adhesion、tight junction以及提升cell migration能力之蛋白之mRNA有差異，例如cell adhesion相關的E-cadherin與Occludin之表現量在HCC827/IR和H1975/AR較少；另外，我們分析lncRNAs表現之差異，發現兩個lncRNAs：CLU及CRNDE高度表現在HCC827/IR和H1975/AR細胞，主要分布在核內，使用DNA gapmers knockdown CLU與CRNDE，探討其在TKI-resistance的角色
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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