1,720,988 research outputs found
Supplementary_materials – Supplemental material for Modulation of the NOP receptor signaling affects resilience to acute stress
No full textSupplemental material, Supplementary_materials for Modulation of the NOP receptor signaling affects resilience to acute stress by Victor A D Holanda, Salvatore Pacifico, Joaquim Azevedo Neto, Luca Finetti, Bruno Lobão-Soares, Girolamo Calo, Elaine C Gavioli and Chiara Ruzza in Journal of Psychopharmacology</p
Primers used in this research work.
No full textThe control of reproductive processes in Rhodnius prolixus involves a variety of neuroactive chemicals. Among these, several studies have suggested that the biogenic amine octopamine (OA), might play an active role in these processes. Here, we investigate the molecular profile of the R. prolixus α adrenergic-like OA receptor 1 (RpOAα1-R) and its role in egg production. Comparative molecular analyses confirm that the RpOAα1-R gene codes for a true OAα1 receptor. The RpOAα1-R transcript is highly expressed in tissues associated with egg production, and after a blood meal, which is the stimulus for full egg production in R. prolixus, the RpOAα1-R transcript is upregulated in the ovaries and spermatheca. After RNAi-mediated RpOAα1-R knockdown, an ovarian phenotype characterized by slow egg development is observed. Furthermore, an altered egg phenotype has been characterized with eggs that are deformed. Interestingly, there is no evidence of disruption in vitellogenin (Vg) synthesis by the fat body or uptake by the oocytes. On the other hand, RpOAα1-R downregulation is correlated with defective choriogenesis in the eggs. These results provide critical information concerning the role of OAα1-R in oogenesis in R. prolixus.</div
<i>R</i>. <i>prolixus</i> OAα1-R sequence: regions chosen for dsRNA are highlighted.
No full textR. prolixus OAα1-R sequence: regions chosen for dsRNA are highlighted.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Hydropathy profile of the predicted RpOAα1-R amino acid sequence.
No full textHydropathy profile of the predicted RpOAα1-R amino acid sequence.</p
Fluorescent in situ hybridization analysis of <i>RpOAα1-R</i> transcript in the reproductive system of unfed <i>R</i>. <i>prolixus</i> adult females.
No full textAnatomy of the female R. prolixus reproductive system (A). Localization of the RpOAα1-R transcript across the reproductive system. CO: common oviduct; LO: lateral oviduct (B). Localization of the RpOAα1-R transcript in the spermatheca (C), tropharium (D), and follicle (E). FISH experiments were repeated twice, using 3 reproductive systems in each replicate.</p
Controls for fluorescent in situ hybridization of RpOAα1-R transcript in the reproductive system of <i>R</i>. <i>prolixus</i> adult females.
No full textControls for fluorescent in situ hybridization of RpOAα1-R transcript in the reproductive system of R. prolixus adult females.</p
<i>R</i>. <i>prolixus</i> OAα1-R sequence (accession number OR248009).
No full textR. prolixus OAα1-R sequence (accession number OR248009).</p
Molecular features of <i>R</i>. <i>prolixus</i> OAα1-R.
No full textThe control of reproductive processes in Rhodnius prolixus involves a variety of neuroactive chemicals. Among these, several studies have suggested that the biogenic amine octopamine (OA), might play an active role in these processes. Here, we investigate the molecular profile of the R. prolixus α adrenergic-like OA receptor 1 (RpOAα1-R) and its role in egg production. Comparative molecular analyses confirm that the RpOAα1-R gene codes for a true OAα1 receptor. The RpOAα1-R transcript is highly expressed in tissues associated with egg production, and after a blood meal, which is the stimulus for full egg production in R. prolixus, the RpOAα1-R transcript is upregulated in the ovaries and spermatheca. After RNAi-mediated RpOAα1-R knockdown, an ovarian phenotype characterized by slow egg development is observed. Furthermore, an altered egg phenotype has been characterized with eggs that are deformed. Interestingly, there is no evidence of disruption in vitellogenin (Vg) synthesis by the fat body or uptake by the oocytes. On the other hand, RpOAα1-R downregulation is correlated with defective choriogenesis in the eggs. These results provide critical information concerning the role of OAα1-R in oogenesis in R. prolixus.</div
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