1,720,963 research outputs found
Ultrasonographic examination and cross sectional area (CSA) of main tendons of shoulder and elbow in German Shepherd dogs
No full textEsame ultrasonografico e Cross Sectional Area (CSA) dei pricinpali tendini di spalla e gomito nel cane pastore tedesco: studio preliminare su 10 soggetti
No full textStudio dei fattori che influenzano l’ecogenicita’ media dei tendini nel cane pastore tedesco adulto
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Surgical repair of Achilles tendon rupture in dogs: A review of the literature, a case report and new perspectives
Full text linkAchilles tendon rupture in the dog is a common traumatic lesion. An accurate evaluation of etiology, grade of the lesion with or without loss of tendon substance, and time elapsed from the trauma can guide the surgeon in choosing the best surgical technique. Moreover, the healing process after a tendon injury is usually difficult and uncertain because the prognosis is influenced by extent of trauma, time elapsed between the trauma and its treatment and low tendon vascularization. However, the rapid development of tissue engineering and regenerative medicine could soon result in the development of scaffolds with bioactive proteins that can direct the healing process toward complete tendon regeneration without fibrotic tissue development
Greening the Production of Indigo Blue Exploiting Light and a Recombinant Synechocystis sp. PCC6803 Strain Expressing the Enzyme mFMO
No full textCyanobacteria are emerging as interesting cell factories, offering the significant advantage of their in-built photosynthetic machinery, which generates NADPH to support redox biocatalysis. In this study, we assessed the potential of the cyanobacterium Synechocystis sp. PCC6803 in producing the dye indigo by light-driven whole-cell biotransformation using indole as a starting compound. A stable transgenic strain expressing a flavin-containing monooxygenase from Methylophaga aminisulfidivorans (mFMO) was engineered, enabling light-dependent indigo production. Upon optimising conditions, effective biotransformations could be performed, resulting in 112 mg/L indigo (86% conversion of the furnished indole). Additionally, we present a method for the recovery of the secreted dye directly from the growth medium through solid-phase absorption on polyamide nets. Overall, the effectiveness and sustainability of the biotransformation in Synechocystis sp. PCC6803 performed at the laboratory scale provide a strong basis for further exploring the applicability of the process
A new thermophilic ene‐reductase from the filamentous anoxygenic phototrophic bacterium Chloroflexus aggregans
Full text linkAiming at expanding the biocatalytic toolbox of ene‐reductase enzymes, we decided to explore photosynthetic extremophile microorganisms as unique reservoir of (new) biocatalytic ac-tivities. We selected a new thermophilic ene‐reductase homologue in Chloroflexus aggregans, a pe-culiar filamentous bacterium. We report here on the functional and structural characterization of this new enzyme, which we called CaOYE. Produced in high yields in recombinant form, it proved to be a robust biocatalyst showing high thermostability, good solvent tolerance and a wide range of pH optimum. In a preliminary screening, CaOYE displayed a restricted substrate spectrum (with generally lower activities compared to other ene‐reductases); however, given the amazing metabolic ductility and versatility of Chloroflexus aggregans, further investigations could pinpoint pecu-liar chemical activities. X‐ray crystal structure has been determined, revealing conserved features of Class III (or thermophilic‐like group) of the family of Old Yellow Enzymes: in the crystal pack-ing, the enzyme was found to assemble as dimer even if it behaves as a monomer in solution. The description of CaOYE catalytic properties and crystal structure provides new details useful for en-larging knowledge, development and application of this class of enzymes
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Metabolic and enzymatic engineering strategies for polyethylene terephthalate degradation and valorization
Full text linkPolyethylene terephthalate (PET) is one of the most marketed aromatic polyesters in the world with an annual demand in 2022 of approximately 29 million metric tons, expected to increase by 40% by 2030. The escalating volume of PET waste and the current inadequacy of recycling methods have led to an accumulation of PET in the terrestrial ecosystem, thereby posing significant global health risks. The pressing global energy and environmental issues associated with PET underscore the urgent need for "upcycling" technologies. These technologies aim to transform reclaimed PET into higher-value products, addressing both energy concerns and environmental sustainability. Enzyme-mediated biocatalytic depolymerization has emerged as a potentially bio-sustainable method for treating and recycling plastics. Numerous plastic-degrading enzymes have been identified from microbial origins, and advancements in protein engineering have been employed to modify and enhance these enzymes. Microbial metabolic engineering allows for the development of modified microbial chassis capable of degrading PET substrates and converting their derived monomers into industrial relevant products. In this review, we describe several engineering approaches aiming at enhancing the performances of PET-degrading enzymes and we present the current metabolic engineering strategies adopted to bio-upcycle PET into high-value molecules
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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