1,720,953 research outputs found
Evaluation of the effects of a nonspecific phage cocktail on the biofilm and growth of Stutzerimonas stutzeri
Full text linkA corrosão microbiologicamente induzida (CMI) representa um desafio econômico e ambiental relevante, especialmente nas indústrias de petróleo e gás, onde a formação de biofilmes por microrganismos acelera a deterioração de superfícies metálicas. Entre as espécies bacterianas associadas a esse fenômeno destaca-se Stutzerimonas stutzeri, bactéria de origem marinha com capacidade de formar biofilmes e degradar hidrocarbonetos, comprometendo a integridade de estruturas metálicas e a qualidade do petróleo. Estratégias inovadoras de controle, como o uso de bacteriófagos, têm se mostrado alternativas promissoras aos biocidas químicos, devido à sua especificidade, à produção de enzimas que degradam a matriz extracelular e ao potencial de aplicação em coquetéis combinados. Neste trabalho, foram avaliados os efeitos de fagos isolados e de um coquetel fágico inespecífico sobre o crescimento e a formação de biofilme de S. stutzeri. Ensaios foram conduzidos em microplacas MBEC (Minimum Biofilm Eradication Concentration) e em tanques piloto contendo cupons de aço carbono, com avaliação do crescimento bacteriano, biomassa total, morfologia do biofilme por Microscopia Eletrônica de Varredura (MEV), rugosidade superficial por perfilometria óptica e observação de células aderidas por microscopia de fluorescência. Os resultados mostraram que, embora o coquetel fágico não tenha causado uma redução significativa no crescimento de células planctônicas, foi capaz de prevenir a formação de biofilme, especialmente em uma Multiplicidade de Infecção (MOI) de 10. A análise de perfilometria óptica indicou menor rugosidade nos cupons tratados, sugerindo menor presença de biofilme. A microscopia de fluorescência também revelou células mais espaçadas nos cupons tratados com o coquetel no MOI 10. Esses resultados sugerem o potencial biotecnológico do coquetel fágico para o controle do biofilme de S. stutzeri e para a mitigação da CMI em ambientes industriais, como a exploração de petróleo. Palavras-chave: corrosão microbiologicamente induzida; bacteriófagos; fagoterapia; superfícies metálicasMicrobiologically Induced Corrosion (MIC) represents a significant economic and environmental challenge, especially in the oil and gas industries, where the formation of biofilms by microorganisms accelerates the deterioration of metallic surfaces. Among the bacterial species associated with this phenomenon, Stutzerimonas stutzeri stands out. This is a marine bacterium with the capacity to form biofilms and degrade hydrocarbons, thus compromising the integrity of metallic structures and oil quality. Innovative control strategies, such as the use of bacteriophages, have proven to be promising alternatives to chemical biocides due to their specificity, their production of enzymes that degrade the extracellular matrix, and their potential for application in combined cocktails. In this work, the effects of isolated phages and a non-specific phage cocktail on the growth and biofilm formation of S. stutzeri were evaluated. Assays were conducted in MBEC (Minimum Biofilm Eradication Concentration) microplates and in pilot tanks containing carbon steel coupons, with assessment of bacterial growth, total biomass, biofilm morphology by Scanning Electron Microscopy (SEM), surface roughness by optical profilometry, and observation of adhered cells by fluorescence microscopy. The results showed that, although the phage cocktail did not cause a significant reduction in the growth of planktonic cells, it was capable of preventing biofilm formation, especially at a Multiplicity of Infection (MOI) of 10. Optical profilometry analysis indicated lower roughness on the treated coupons, suggesting a lower presence of biofilm. Fluorescence microscopy also revealed more spaced-out cells on the coupons treated with the cocktail at MOI 10. These results suggest the biotechnological potential of the phage cocktail for the control of S. stutzeri biofilm and for the mitigation of MIC in industrial environments, such as oil exploration. Keywords: microbiologically induced corrosion (MIC); bacteriophages; phage therapy; metallic surfaces.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico - (CNPQ)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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