2,703 research outputs found
The complete mitochondrial genomes of Schistosoma haematobium and Schistosoma spindale and the evolutionary history of mitochondrial genome changes among parasitic flatworms
No full textComplete mitochondrial genome sequences for the schistosomes Schistosoma haematobium and Schistosoma. spindale have been characterized. S. haematobium is the causative agent of urinary schistosomiasis in humans and S. spindale uses ruminants as its definitive host; both are transmitted by freshwater snail intermediate hosts. Results confirm a major gene order rearrangement among schistosomes in all traditional Schistosoma species groups other than Schistosoma japonicum; i.e., species groups S. mansoni, S. haematobium, and S. indicum. These data lend support to the 'out of Asia' (East and Southeast Asia) hypothesis for Schistosoma. The gene order change involves translocation of atp6-nad2-trnA and a rearrangement of nad3-nad1 relative to other parasitic flatworm mt genomes so far sequenced. Gene order and tRNA secondary structure changes (loss and acquisition of the DHU and/or TPsiC arms of trnC, trnF, and trnR) between mitochondrial genomes of these and other (digenean and cestode) flatworms were inferred by character mapping onto a phylogeny estimated from nuclear small subunit rRNA gene sequences of these same species, in order to find additional rare genomic changes suitable as synapomorphies. Denser and wider taxon sampling of mt genomes across the Platyhelminthes will validate these putative characters
Interview: Anne-Marie Fortier
No full textThis paper is an edited version of an email interview conducted by Debra Ferreday and Adi Kuntsman with Anne-Marie Fortier, the author of Multicultural Horizons: Diversity and the Limits of the Civil Nation (Routledge, 2008). Fortier’s work has been informative in the development of some of the arguments explored in this special issue; in their conversation Ferreday and Kuntsman asked her to comment on the ideas of haunting, racial imaginaries, nostalgia, national anxieties, political feelings and hopes for the future
E. Barrett Prettyman papers
No full textE. Barrett Prettyman, Jr. (b. 1925), was Katherine Anne Porter's lawyer and close friend from the mid-1960s until her death in 1980. He has been in private practice in Washington, D.C., since 1955, and a partner with the prominent firm of Hogan and Hartson since 1964. He is the author of Death and the Supreme Court. His papers include personal letters and business correspondence relating to Porter. During the lifetime of Mr. Prettyman, no one may quote from these papers unless authorized in writing by him. In addition, researchers may not consult the portion of these papers donated in 1997 without Mr. Prettyman's written permission
'The cracked mirror': Anne Sexton's poetics of self-representation
No full textThis thesis re-evaluates the work of the poet Anne Sexton (1928-1974), concentrating, in particular, on the indeterminacies, contradictions and aporia which it finds to be characteristic of her ostensibly frank and self-revelatory writing. The study is based on a close textual
analysis of Sexton's writing, is informed by oststructuralist theories, and is sustained by an
examination and discussion of archive collections of her previously unpublished papers. In seeking an understanding of Sexton's poetics, the thesis identifies and interrogates the strategies of denial and obfuscation apparent in her own explication of her work - principally, by scrutiny of the unpublished, and previously unresearched, drafts of a series of lectures
which she delivered in 1972. Chapters One and Two consider the origins of `confessional' or - Sexton's preferred term - 'personal' poetry and reassess her place within contemporary poetry. They suggest that
Sexton's writing is engaged in a process of negotiation and contestation, both with the boundaries and expectations of confessionalism, and with the strictures of T. S. Eliot's theory of `impersonality'. In support of these arguments, Chapter Two offer a reading of Sexton's
little-known poem, `Hurry Up Please It's Time', alongside its intertext, Eliot's The Waste Land. Chapter Three reassesses received views of the supposedly beneficial interrelationship between confessional speaker and reader. It examines Sexton's appropriation of dramatic
masks and personae and her use of metaphors of striptease and prostitution, and suggests that these are employed simultaneously to appease and to repel an intrusive audience. Similarly, Chapters Four and Five trace Sexton's problematisation of two previously-accepted tenets of confessional poetry: its status as autobiography and its truthfulness, drawing attention to the techniques employed in order to give the impression of both. Chapter Six considers Sexton's
problematic engagement with a language which is not malleable, transparent, and referential but, rather, is experienced as uncooperative and occlusive. Finally, the thesis recuperates Sexton from the common charge of narcissism, arguing that it is the writing, rather than the poet, which is self-reflexive and self-conscious. In this respect, it concludes that her work - perhaps unexpectedly - anticipates many of the tendencies of postmodernist writing
Anne E. Streaty Wimberly, Interdenominational Theological Center, April 23, 2020
No full textStatement submitted by Anne E. Streaty Wimberly, Director, Youth Hope-Builders Academy (YHBA Theological Program for High School Youth) and the Connecting With Hope Innovation Hub (CWHIH)Young Adult Ministry Initiative centered in twenty-three churches in and beyond the Atlanta Metro Area. Both YHBA and CWHIH are outreach programs of Interdenominational Theological Center (ITC). CWHIH is currently funded by Lilly Endowment Incorporated
Anne E. Streaty Wimberly, Interdenominational Theological Center, April 23, 2020
No full textStatement submitted by Anne E. Streaty Wimberly, Director, Youth Hope-Builders Academy (YHBA Theological Program for High School Youth) and the Connecting With Hope Innovation Hub (CWHIH)Young Adult Ministry Initiative centered in twenty-three churches in and beyond the Atlanta Metro Area. Both YHBA and CWHIH are outreach programs of Interdenominational Theological Center (ITC). CWHIH is currently funded by Lilly Endowment Incorporated
Anne-Marie Fortier in conversation with Debra Ferreday and Adi Kuntsman
No full textThis paper is an edited version of an email interview conducted by Debra Ferreday and Adi Kuntsman with Anne-Marie Fortier, the author of Multicultural Horizons: Diversity and the Limits of the Civil Nation (Routledge 2008). Fortier’s work has been informative in the development of some of the arguments explored in this special issue; in their conversation Ferreday and Kuntsman asked her to comment on the ideas of haunting, racial imaginaries, nostalgia, national anxieties, political feelings and hopes for the future
Open access self-archiving: An author study
No full textThis, our second author international, cross-disciplinary study on open access had 1296 respondents. Its focus was on self-archiving. Almost half (49%) of the respondent population have self-archived at least one article during the last three years. Use of institutional repositories for this purpose has doubled and usage has increased by almost 60% for subject-based repositories. Self-archiving activity is greatest amongst those who publish the largest number of papers. There is still a substantial proportion of authors unaware of the possibility of providing open access to their work by self-archiving. Of the authors who have not yet self-archived any articles, 71% remain unaware of the option. With 49% of the author population having self-archived in some way, this means that 36% of the total author population (71% of the remaining 51%), has not yet been appraised of this way of providing open access. Authors have frequently expressed reluctance to self-archive because of the perceived time required and possible technical difficulties in carrying out this activity, yet findings here show that only 20% of authors found some degree of difficulty with the first act of depositing an article in a repository, and that this dropped to 9% for subsequent deposits. Another author worry is about infringing agreed copyright agreements with publishers, yet only 10% of authors currently know of the SHERPA/RoMEO list of publisher permissions policies with respect to self-archiving, where clear guidance as to what a publisher permits is provided. Where it is not known if permission is required, however, authors are not seeking it and are self-archiving without it. Communicating their results to peers remains the primary reason for scholars publishing their work; in other words,
researchers publish to have an impact on their field. The vast majority of authors (81%) would willingly comply with a mandate from their employer or research funder to deposit copies of their articles in an institutional or subject-based repository. A further 13% would comply reluctantly; 5% would not comply with such a mandate
Biomphalaria glabrata transcriptome: cDNA microarray profiling identifies resistant- and susceptible-specific gene expression in haemocytes from snail strains exposed to Schistosoma mansoni
No full text© 2008 Lockyer et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Background - Biomphalaria glabrata is an intermediate snail host for Schistosoma mansoni, one of the important schistosomes infecting man. B. glabrata/S. mansoni provides a useful model system for investigating the intimate interactions between host and parasite. Examining differential gene expression between S. mansoni-exposed schistosome-resistant and susceptible snail lines will identify genes and pathways that may be involved in snail defences.
Results - We have developed a 2053 element cDNA microarray for B. glabrata containing clones from ORESTES (Open Reading frame ESTs) libraries, suppression subtractive hybridization (SSH) libraries and clones identified in previous expression studies. Snail haemocyte RNA, extracted from parasite-challenged resistant and susceptible snails, 2 to 24 h post-exposure to S. mansoni, was hybridized to the custom made cDNA microarray and 98 differentially expressed genes or gene clusters were identified, 94 resistant-associated and 4 susceptible-associated. Quantitative PCR analysis verified the cDNA microarray results for representative transcripts. Differentially expressed genes were annotated and clustered using gene ontology (GO) terminology and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis. 61% of the identified differentially expressed genes have no known function including the 4 susceptible strain-specific transcripts. Resistant strain-specific expression of genes implicated in innate immunity of invertebrates was identified, including hydrolytic enzymes such as cathepsin L, a cysteine proteinase involved in lysis of phagocytosed particles; metabolic enzymes such as ornithine decarboxylase, the rate-limiting enzyme in the production of polyamines, important in inflammation and infection processes, as well as scavenging damaging free radicals produced during production of reactive oxygen species; stress response genes such as HSP70; proteins involved in signalling, such as importin 7 and copine 1, cytoplasmic intermediate filament (IF) protein and transcription enzymes such as elongation factor 1α and EF-2.
Conclusion - Production of the first cDNA microarray for profiling gene expression in B. glabrata provides a foundation for expanding our understanding of pathways and genes involved in the snail internal defence system (IDS). We demonstrate resistant strain-specific expression of genes potentially associated with the snail IDS, ranging from signalling and inflammation responses through to lysis of proteinacous products (encapsulated sporocysts or phagocytosed parasite components) and processing/degradation of these targeted products by ubiquitination.The Wellcome Trus
Anne Shirley do século XXI: a adaptação da personagem na série Anne With An E
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-Graduação em Estudos da Tradução, Florianópolis, 2021.Este é um estudo sobre a personagem Anne Shirley, protagonista de Anne of Green Gables (1908), da autora canadense L. M. Montgomery, tal qual adaptada na série Anne With an E (2017), de Moira Walley-Beckett, produzida pelo canal canadense CBC (Canadian Broadcasting Corporation) e transmitida pela plataforma de streaming Netflix. O presente trabalho tem como objetivo central analisar os principais recursos audiovisuais adotados para a introdução da personagem no episódio-piloto da série, baseando-se em noções de adaptação propostas por Julie Sanders (2006), Deborah Cartmell (1999), Brian McFarlane (1996) e, sobretudo, Linda Hutcheon (2013). Como metodologia para a construção dessa análise, foram selecionados três recursos audiovisuais de adaptação largamente presentes no primeiro episódio da série: flashback, enquadramentos em primeiro plano (ou close) e falas de efeito enunciadas. Também é discutido o possível impacto do streaming na distribuição e recepção do entretenimento contemporâneo ? para abordar esse fenômeno, recorreu-se a estudiosos da área da comunicação, como Chris Anderson (2006), John Fiske (2011), Pierre Levy (2013) e Raymond Williams (2016), entre outros. Esta pesquisa está ancorada, ainda, em parte da vasta fortuna crítica de mais de 100 anos da obra de Montgomery, de modo a fundamentar a hipótese de que a série atualizou a personagem Anne com vistas ao público do século XXI.Abstract: This study approachesthe character of Anne Shirley, protagonist of Anne of Green Gables (1908), by Canadian author L.M. Montgomery, as adapted into the series Anne with an E (2017), by Moira Walley-Beckett, produced by the Canadian channel CBC (Canadian Broadcasting Corporation) and broadcasted by Netflix. This work has as main objective analyzing the main audiovisual resources chosen and applied to the presentation of the character in the series? pilot episode, based on the concepts of adaptation proposed by Julie Sanders (2006), Deborah Cartmell (1999), Brian MacFarlane (1996) and, mainly, Linda Hutcheon (2013). As a methodology to build the analyses three audiovisual resources largely presented at the first episode of the series were selected: flashbacks, close-ups, and eloquent speech. The possible effect of the streaming at the distribution and reception of contemporary entertainment is also discussed ? this phenomenon is understood by gathering ideas from scholars of communication, as Chris Anderson (2006), John Fiske (2011), Pierre Levy (2013) and Raymond Williams (2016), among others. Furthermore, this research is based on some of the extensive critical fortune of more than 100 years of Montgomery?s work, to support the hypothesis that the series updated the character Anne as a means of aiming at 21st century?s audience
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