385 research outputs found
Scanning of the supramolecular organization of the proteins of the extracellular matrix by biophysical techniques
No full text
Broadband Molecular dynamics in tropoelastin and its analogous fragments: influence of pathology on the physical structure
No full textLas Cortes de Castilla bajo el reinado del Emperador Carlos V (1516-1556)
No full textA lo largo del presente trabajo hemos abordado el estudio de las Cortes de Castilla en aquel período en el que presidió la Corona castellana, conjuntamente con su madre la reina Juana (1506-1555) el rey Carlos I (1518-1556), futuro emperador desde 1521. En los inicios de la Edad Moderna las Cortes constituían un órgano de naturaleza eminentemente consultivo para con la autoridad regia, en el que había llegado a residir una importante competencia fiscal, cada vez más mediatizada y controlada por el monarca y sus oficiales. La revuelta de los Comuneros constituye el momento clave en el que la autoridad del reino, representada en las Cortes, y la del rey, con un poder cada vez más personal y exclusivo, llegaron a enfrentarse, en perjuicio de la primera, lo que constituyó el principio del fin de las antiguas Cortes medievales y de su pretensión de convertirse en una cámara parlamentaria.Departamento de Derecho Penal e Historia y Teoría del DerechoGrado en Derech
Circular RNAs: a novel tool in cardiovascular biomarker development?
No full textAs we move towards cardiovascular personalised medicine, the development of novel biomarkers to assist in the decision-making process is a hot topic in translational research. In recent years, the non-coding transcriptome has emerged as a novel source of indicators with clinical applications. A vast number of publications have highlighted the potential of different members of this family as biomarkers, especially microRNAs and long non-coding RNAs. Recent advances in RNA sequencing and bioinformatic analysis have drawn attention to another promising class of non-coding RNAs: circular RNAs (circRNAs). CircRNAs are single-stranded and covalently closed RNA molecules that lack free caps or poly(A) tails. Initially discovered in plant viroids as early as the 1970s, circRNAs were originally considered splicing by-products or background noise. Nonetheless, transcriptome-wide analyses have identified and characterised thousands of circRNAs in diverse human cells, with key functions in the regulation of cellular processes. Indeed, various authors have proposed a causative role in a number of pathological conditions, including cardiovascular disease (CVD). Furthermore, circRNAs have the optimal chemical and biological properties to serve as interesting biomarkers. CircRNAs are free of exonuclease-mediated degradation and have a longer half-life than most linear RNAs, and their expression is cell- and developmental stage- and disease-specific. The presence of circRNAs has been demonstrated in clinical specimens, including whole blood, plasma and serum. Here, we review publications that have evaluated the potential of circRNAs as biomarkers for the diagnosis and prediction of cardiovascular conditions, including atherosclerosis, hypertension, aneurysm and the risk of adverse events. In addition, we discuss current methodological limitations and proposed future steps for their application as clinical indicators.D de Gonzalo-Calvo was a recipient of a Juan de la Cierva-Incorporación grant from the Ministerio de Economía y Competitividad (IJCI-2016-29393). CIBER Cardiovascular (CB16/11/00403 to D de Gonzalo-Calvo and V Llorente-Cortes) is a project of the Instituto de Salud Carlos III.Peer reviewe
Low-Density Lipoprotein Upregulates Low-Density Lipoprotein Receptor-Related Protein Expression in Vascular Smooth Muscle Cells: Possible Involvement of Sterol Regulatory Element Binding Protein-2-Dependent Mechanism
No full textProvenance-based trust for grid computing: Position Paper
No full textCurrent evolutions of Internet technology such as Web Services, ebXML, peer-to-peer and Grid computing all point to the development of large-scale open networks of diverse computing systems interacting with one another to perform tasks. Grid systems (and Web Services) are exemplary in this respect and are perhaps some of the first large-scale open computing systems to see widespread use - making them an important testing ground for problems in trust management which are likely to arise. From this perspective, today's grid architectures suffer from limitations, such as lack of a mechanism to trace results and lack of infrastructure to build up trust networks. These are important concerns in open grids, in which "community resources" are owned and managed by multiple stakeholders, and are dynamically organised in virtual organisations. Provenance enables users to trace how a particular result has been arrived at by identifying the individual services and the aggregation of services that produced such a particular output. Against this background, we present a research agenda to design, conceive and implement an industrial-strength open provenance architecture for grid systems. We motivate its use with three complex grid applications, namely aerospace engineering, organ transplant management and bioinformatics. Industrial-strength provenance support includes a scalable and secure architecture, an open proposal for standardising the protocols and data structures, a set of tools for configuring and using the provenance architecture, an open source reference implementation, and a deployment and validation in industrial context. The provision of such facilities will enrich grid capabilities by including new functionalities required for solving complex problems such as provenance data to provide complete audit trails of process execution and third-party analysis and auditing. As a result, we anticipate that a larger uptake of grid technology is likely to occur, since unprecedented possibilities will be offered to users and will give them a competitive edge
Epigenetic Biomarkers and Cardiovascular Disease: Circulating MicroRNAs
No full text[EN] MicroRNAs (miRNAs) are a class of small noncoding RNA (20-25 nucleotides) involved in gene regulation.
In recent years, miRNAs have emerged as a key epigenetic mechanism in the development and physiology
ofthe cardiovascular system. Thesemolecular species regulate basic functions invirtually all celltypes, and
are therefore directly associated with the pathophysiology of a large number of cardiovascular diseases.
Since their relatively recent discovery in extracellular fluids, miRNAs have been studied as potential
biomarkers of disease.A wide array of studies have proposed miRNAs as circulating biomarkers of different
cardiovascular pathologies (eg, myocardial infarction, coronary heart disease, and heart failure, among
others), which may have superior physicochemical and biochemical properties than the conventional
protein indicators currently used in clinical practice. In the present review, we provide a brief introduction
to the field of miRNAs, paying special attention to their potential clinical application. This includes their
possible role as new diagnostic or prognostic biomarkers in cardiovascular disease[ES] Los microARN (miARN) son una clase de ARN no codificante de pequen˜o taman˜o (20-25 nucleo´tidos) que
participan en la regulacio´n ge´nica. En los u´ ltimos an˜os, los miARN han emergido como un mecanismo
epigene´tico clave en el desarrollo y en la funcionalidad del sistema cardiovascular. Estas especies
moleculares regulan funciones ba´ sicas en pra´ cticamente todos los tipos celulares y por ello esta´n
directamente asociadas con la fisiopatologı´a de un gran nu´ mero de enfermedades cardiovasculares.
Desde su relativamente reciente descubrimiento en fluidos extracelulares, se ha estudiado los miARN
como potenciales biomarcadores de enfermedad. Son numerosos los estudios que proponen a los miARN
como biomarcadores circulantes de diferentes patologı´as cardiovasculares (infarto de miocardio,
enfermedad coronaria o insuficiencia cardiaca, entre otras), incluso con propiedades fisicoquı´micas y
bioquı´micas superiores a los indicadores proteicos convencionales utilizados actualmente en la pra´ ctica
clı´nica. En el presente trabajo se ofrece una breve introduccio´n al campo de los miARN, con especial
atencio´n a su potencial aplicacio´n clı´nica. Esta incluye su posible papel como nuevos biomarcadores de
diagno´ stico o de prono´ stico en la enfermedad cardiovascular.Our study received aid from the Carlos III Health Institute,
which is cofunded with financial assistance from the ERDF
(European Regional Development Fund) (V. Llorente-Corte´ s: FIS
PI14/01729), the Carlos III Health Institute (Cardiovascular
Biomedical Research Network Center: CB16/11/00403), the TV3
Marathon Foundation (V. Llorente-Cortes: 201521-10) and the
Carlos III Health Institute, Ministry of Economy and Competitiveness (E. Iglesias-Gutie´ rrez: DEP2012-39262). D. de Gonzalo-Calvo
has a Sara Borrell contract (CD14/00109) with the Carlos III Health
Institute. D. de Gonzalo-Calvo and V. Llorente-Corte´ s are members
of the European network Cardiolinc.Peer reviewe
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