98 research outputs found
FIGURE 2 in Rosellinia qiongensis sp. nov., R. verticillata sp. nov. and a new record of R. lamprostoma from China
No full textFIGURE 2. Rosellinia lamprostoma (GMB0086) A. Host. B. Stromata on host substrate C. Close-up of stroma D. Transverse section trough stroma E. Vertical section through stroma F–H. Asci I. Ascus apical ring in Melzer's reagent. J, K. Ascospores L. Ascospores in black ink M. Culture on PDA. Scale bars: B = 1 mm, C–E = 200 μm, F–L = 10 μm.Published as part of Long, Sihan, Pi, Yinhui, Wu, Youpeng, Liu, Lili, Zhang, Xu, Long, Qingde, Lin, Yan, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N. & Li, Qirui, 2022, Rosellinia qiongensis sp. nov., R. verticillata sp. nov. and a new record of R. lamprostoma from China, pp. 287-300 in Phytotaxa 552 (5) on page 292, DOI: 10.11646/phytotaxa.552.5.2, http://zenodo.org/record/679657
Large sets of extended directed triple systems with odd orders
No full textAbstractFor three types of triples, unordered, cyclic and transitive, the corresponding extended triple, extended triple system and their large set are introduced. The spectrum of LEDTS(v) for even v has been given in our paper (Liu and Kang (2009) [9]). In this paper, we shall discuss the existence problem of LEDTS(v) for odd v and give the almost complete conclusion: there exists an LEDTS(v) for any positive integer v≠4 except possible v=95,143,167,203,215
Author Correction: Caspase-11 signaling enhances graft-versus-host disease (Nature Communications, (2019), 10, 1, (4044), 10.1038/s41467-019-11895-2)
No full text© 2020, The Author(s). In the original version of this Article, the following author affiliations were inadvertently switched. Affiliation of Qingde Wang and Timothy R. Billiar should be: “Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, 15213 USA”. Haichao Wang\u27s affiliation should be: “The Feinstein Institute for Medical Research, Northwell Health, 350 Community Drive, Manhasset, NY11030, USA”. The errors have been corrected in the HTML and PDF versions of this article
Large sets of extended directed triple systems with even orders
No full textAbstractFor three types of triples: unordered, cyclic and transitive, the corresponding extended triple, extended triple system and their large sets are introduced. The existence of LESTS(υ) and LEMTS(υ) were completely solved. In this paper, we shall discuss the existence problem of LEDTS(υ) and give the following conclusion: there exists an LEDTS(υ) for any even υ except υ=4. The existence of LEDTS(υ) with odd order υ will be discussed in another paper, we are working at it
Molecular and physical determinants of fibrinogen-dependent platelet aggregation and adhesion in flow
No full textFibrinogen (Fg) mediates platelet aggregation and adhesion to artificial surfaces by interacting with its receptor, glycoprotein IIb and IIIa complex (GPIIbIIIa, or integrin alphaIIbbeta 3), on the platelet membrane. Considerable evidence has demonstrated that the (H12) on the gamma chain carboxyl terminus is required for the binding of Fg from solution to activated platelet GPIIbIIIa, while the RGD sites, the universal integrin recognition domain on adhesive ligands, are not involved. In this study, using recombinant Fg, well-defined Fg plasmin digestion fragments, and specific monoclonal anit-Fg antibodies, we demonstrated that the same sequence, the H12, or more precisely, the AGDV on the extreme carboxyl terminus of the gamma chain (gamma408--411), is also required for platelet-bound Fg to support platelet aggregation (crosslinking), thus experimentally verifying the "two sticky ends" theory of Fg-mediated platelet aggregation The RGD-containing domains on the Aalpha chains are not involved in aggregation. The AGDV sequence on the gamma chain carboxyl terminus is also necessary and sufficient for activated platelets to adhere to surface-adsorbed Fg, while the RGD sequences we similarly not required. A receptor induced binding site (RIBS), the Fg RIBS-I site (gamma373--385), on Fg either bound to its GPIIbIIIa-receptor or on a surface, is not directly involved in interactions between platelet GPIIbIIIa and immobilized Fg. The inhibitory effects of the anti-Fg-RIBS-I antibody are due to steric hindrance of the accessibility of the AGDV site to platelet GPIIbIIIa. Thus, the extreme carboxyl terminus of the gamma chain is the only site in both fluid and solid phase Fg that is involved in platelet GPIIbIIIa-Fg interactions.Though resting platelets are able to adhere to surface-bound Fg, this adhesion efficiency is much lower than that of the adhesion of the activated platelets. The adhesion efficiency of both resting and activated platelets to surface-adsorbed Fg decreases with increasing shear rate from 100 s -1 to 2,000 s-1. However, the decrease of the adhesion efficiency of the resting platelets is more marked than the decrease of the adhesion efficiency of the activated ones. Thus, the higher the shear rates, the larger the difference in the adhesion efficiencies between resting and activated platelets. However, due to the higher collision frequencies at higher shear rates, the adhesion of resting platelets was maintained at a similar level from shear rates of 300--2,000 s-1, while the adhesion of activated platelets kept increasing from 100 s -1 to 2,000 s-1. These data indicate that platelet activation is an efficient regulation pathway for platelet adhesion to surfaces
Rosellinia verticillata S. H. Long & Q. R. Li 2022, sp. nov.
No full textRosellinia verticillata S.H. Long & Q.R. Li sp. nov. (Fig. 4) MycoBank No: MB 842287 Holotype: — CHINA, Guizhou Province, Tongren, Fanjingshan Nature Reserve (27°53′44.77″N, 108°43′52.69″E), elev. 989 m, dead branch of Litsea verticillata Hance, 14 October 2020, S. H. Long and Lili Liu FJS19 (GMB0084, holotype, ex-type GMBC0084; KUN-HKAS 122639, isotype). Saprobic on dead twig of L. verticillata. Sexual morph Subiculum felted, brown, restricted to rim around stromata, evanescent. Stromata 630–735 µm high × 525–700 µm diam. (av. = 679 × 628 µm, n = 20), superficial, scattered to gregarious, solitary, globose, with a conical pointed top, dark, shiny, carbonaceous. Ostioles black, papillate. Ectostroma 80–100 µm thick, black, carbonaceous. Entostroma disappearing at maturity. Perithecia filling entirely the stroma cavity or partly collapsed. Asci 173. 5–241.5 × 18.5–39 µm (av. = 212.5 × 30 µm, n = 30), 8-spored, unitunicate, cylindrical, short pedicellate, apically rounded, with a long J+, barrel-shaped apical apparatus in Melzer’s reagent, 11.5–15.5 µm high, 7–8 µm wide. Ascospores 42–62 × 6.5–12 µm (av. = 55 × 9.3 µm, n = 30), overlapping uniseriate or biseriate, fusiform, ends rounded, hyaline when immature, slight brown to brown at maturity, straight to curved, with a germ slit running the entire length of the spores, entirely surrounded by a thin slimy sheath visible in water and in black ink, lacking appendages. Asexual morph Undetermined. Culture characteristics: —Ascospores germinated on PDA within 24 hours. Colonies on PDA were white when young, became pale brown, dense, but thinning towards edge, medium dense, white from above, reverse side white at margin, flesh to pale brown at centre. No pigmentation was produced on PDA medium. No conidia were observed on PDA or OA media. Habitat/Distribution: —Known to inhabit dead wood, Guizhou Province, China. Etymology: —Refers to its host species, L. verticillata Other material examined: — CHINA, Guizhou Province, Ya Mu Gou Scenic Area (27°54′42.83″N, 108°42′9.08″E), altitude: 1058 m, dead branch of unidentified plant, 15 October 2020, S. H. Long, FJS45 (GMB0085, KUN-HKAS 122641, living culture GMBC0085). Additional sequences: —GMB0084 (LSU: OM001207) GMB0085 (LSU: OM001208). Note: —In the phylogenetic tree (Fig. 1), R. verticillata clustered together with R. tetrastigmae Q.R. Li & J.C. Kang. However, the ascospores of R. verticillata are smaller than those of R. tetrastigmae (42.0–62.0 × 6.7–12.0 µm vs. 72.5–111.5 × 12.5–19.5 µm), and Rosellinia verticillata (GMB0082) ascospores are completely surrounded by a thin slimy sheath, but the R. tetrastigmae only has the slime at the ends (Xie et al. 2019). Moreover, stromata of R. verticillata are larger than those of R. tetrastigmae (630–735 µm high × 525–700 µm diam. vs. 300–500 mm high × 400–600 mm diam.). And R. verticillata belongs to R. emergens group (Petrini 2013) but it is different from the species which have whole spore length germ silt in this group for R. verticillata ascospores are completely surrounded by a thin slimy sheath.Published as part of Long, Sihan, Pi, Yinhui, Wu, Youpeng, Liu, Lili, Zhang, Xu, Long, Qingde, Lin, Yan, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N. & Li, Qirui, 2022, Rosellinia qiongensis sp. nov., R. verticillata sp. nov. and a new record of R. lamprostoma from China, pp. 287-300 in Phytotaxa 552 (5) on pages 295-297, DOI: 10.11646/phytotaxa.552.5.2, http://zenodo.org/record/679657
Rosellinia qiongensis S. H. Long & Q. R. Li 2022, sp. nov.
No full textRosellinia qiongensis S.H. Long & Q.R. Li sp. nov. (Fig. 3) MycoBank No: MB 842285 Holotype: — CHINA, Hainan Province, Qiongzhong County (19°7′8.60″N, 109°7′8.60″E), elev. 177 m, on dead branch of bamboo, 13 November 2020, S.H. Long and Lili Liu QZ 100-1 (GMB0082, holotype; ex-type GMBC0082); ibid (KUN-HKAS 122640, isotype). Saprobic on a dead branch of bamboo. Sexual morph Subiculum felted cream-colored to light brown, restricted to rim around stromata, persistent. Stromata 560–725 µm high × 525–635 µm diam. (av. = 639 × 582 µm, n = 20), superficial, scattered to gregarious, solitary, globose, with a conical pointed top, dark brown to black, shiny, carbonaceous. Ostioles black, finely to coarsely papillate. Ectostroma 60–80 µm thick, black, carbonaceous. Entostroma black, confined to base. Perithecia not collapsed in the stroma cavity. Asci 108.5–141.5 × 11–24 µm (av. = 126 × 15.5 µm, n = 30) 8-spored, unitunicate, cylindrical, short pedicellate, apically rounded, with a long barrel-shaped, apically flattened, basally attenuated or not, J + apical apparatus in Melzer’s reagent, 6.0–6.8 µm high, 6.6–9.9 µm wide. Ascospores 24.5–31 × 5–8 µm (av. = 27.2 × 6.4 µm, n = 30), biseriate, fusiform, ends rounded, hyaline when immature, slight brown to brown at maturity, straight to curved, with three, 3–9 µm, straight germ slits, without slimy sheathes or appendages. Asexual morph Undetermined. Culture characteristics: —Ascospores germinated on PDA within 24 hours. Colonies on PDA were white when young, became pale brown, dense, but thinning towards the edge, medium dense, white from above, reverse sides were white at the margin, flesh to pale brown at centre, no pigmentation were produced on PDA medium, no conidia were observed on PDA or OA media. Habitat/Distribution: —Known to inhabit dead wood, Hainan Province, China. Etymology: —Refers to Qiong, the abbreviation of Hainan province, where the type specimen was collected. Other material examined: — CHINA, Hainan Province, Qiongzhong County (19°7′8.10″N, 109°52′21.36″E), altitude: 177 m, dead branch of bamboo, 13 November 2020, S.H. Long, QZ180 (GMB0083, paratype, ex-paratype GMBC0083). Additional sequences: —GMB0082 (LSU: OM001205); GMB0083 (LSU: OM001206). Notes: —Our new strains of Rosellinia qiongensis accommodate as the sister clade to R. pervariabilis Q.R. Li & J.C. Kang in the phylogenetic tree with high statistical supports (100/0.99) (Fig. 1). Their ascospores are similar in shape and both are light brown in color, but R. qiongensis had three germ slits on ascospores while R. pervariabilis are lacking them. Moreover, the size of ascospores of R. qiongensis is larger than that of R. pervariabilis (24.5–31× 5–8 µm vs. 19.5–24.5 × 4–5 µm) (Xie et al. 2019). Rosellinia qiongensis belongs to Rosellinia emergens group and it is also reminiscent of R. dolichospora Syd. & P. Syd. and R. patilii L.E. Petrini, which has fusiform, brown ascospores with three short germ slits (Petrini 2013). However, R. qiongensis differs from R. dolichospora and R. patilii by its smaller ascospores (24.5–31 × 5–8 µm in R. qiongensis, 29–33 × 6.3– 7.7 µm in R. dolichospora, and 38.6–45.4 × 7.2– 9.8 µm in R. patilii) (Petrini 2013).Published as part of Long, Sihan, Pi, Yinhui, Wu, Youpeng, Liu, Lili, Zhang, Xu, Long, Qingde, Lin, Yan, Kang, Yingqian, Kang, Jichuan, Wijayawardene, Nalin N. & Li, Qirui, 2022, Rosellinia qiongensis sp. nov., R. verticillata sp. nov. and a new record of R. lamprostoma from China, pp. 287-300 in Phytotaxa 552 (5) on pages 293-295, DOI: 10.11646/phytotaxa.552.5.2, http://zenodo.org/record/679657
Restricted t-wise L-intersecting families on set systems
No full textAbstractLet L={λ1,…,λs} be a set of s non-negative integers with λ1<λ2<⋯<λs, and let t≥2. A family F of subsets of an n-element set is called t-wise L-intersecting if the cardinality of the intersection of any t distinct members in F belongs to L. We give the following improvement to the Füredi–Sudakov theorem. Let t≥3 and F be a t-wise L-intersecting family of subsets of [n]. Then, for |⋂F∈FF|<λ1, |F|=o(ns); for |⋂F∈FF|≥λ1, and n sufficiently large, |F|≤k+s−1s+1n−λ1s+∑i≤s−1n−λ1i. We also give a sharp upper bound for the size of a k-uniform t-wise L-intersecting family when s=1
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