17 research outputs found
Candidate High-Resolution Mass Spectrometry-Based Reference Method for the Quantification of Procalcitonin in Human Serum Using a Characterized Recombinant Protein as a Primary Calibrator
No full textDataset related to Huu-Hien Huynh, Vincent Delatour, Maxence Derbez-Morin, Qinde Liu, Amandine Boeuf, and Joëlle Vinh, (2022) Candidate High-Resolution Mass Spectrometry-Based Reference Method for the Quantification of Procalcitonin in Human Serum Using a Characterized Recombinant Protein as a Primary Calibrator. Anal. Chem. 2022, 94, 10, 4146–4154.This project (18HLT03 SEPTIMET) has received funding from the EMPIR programme co-financed by the Participating States and from the European Union's Horizon 2020 research and innovation programme
Preparation of Two Luminescent Complexes: AlIII(8-hydroxyquinolinolato)3 and EuIII(thenoyltrifluoroacetonato)3(1,10-phenanthroline)
No full textLuminescent Atropisomeric N,N-Chelating Ligands from Copper-Catalyzed One-Pot C−N and C−C Coupling Reactions
No full textNew atropisomeric N,N-chelating ligands with a 3,3‘-bis[2-(2‘-py)-indolyl] unit have been achieved via one-pot reactions that involve the formation
of multiple C−N bonds between an indolyl and a brominated benzene and the indolyl 3,3‘-C−C coupling. The new ligands display distinct blue
intramolecular excimer emission (λmax = 445 nm). Cu(I) ions bind to the new N,N-chelate ligands with a homochiral selectivity. The complex
[Cu(bpib)2][BF4] (bpib = bis{3,3‘-[N-phenyl-2-(2‘-py)-indolyl]}) crystallizes as chiral crystals, thus allowing enantiomeric separation
Response to Letter to the Editor regarding “Achieving comparability with IFCC reference method for the measurement of hemoglobin A1c by use of an improved isotope-dilution mass spectrometry method”
No full textElectro-mass olfactory multi-sensor (EMMS)
No full textFor an olfactory sensor or electronic nose, the task is not only to detect the object concentration, but also to recognize it. It is well known that all the elements can be identified by their charge to mass ratio e(+)/m. We tried to imitate this principle for molecular recognition. Two kinds of sensors are used simultaneously in testing. One is quartz crystal microbalance (QCM) for detecting the change in mass, the other is interdigital electrode (IE) for detecting the change in conduction, as an electro-mass multi-sensor (EMMS). in this paper, the principle and the feasibility of this method are discussed. The preliminary results on the recognition of alcohol by EMMS coated with lipids are presented. Meanwhile, the multi-sensor can also be used as an instrument for research on some physico-chemistry problems. The change in conduction of coated membrane caused by one absorbed molecule is reported. It is found that when a QCM is coated with membrane, it still obeys the relationship Delta F (frequency change of QCM) = K Delta m (mass change of absorbed substance) and the proportional coefficient, K, depends not only on quartz properties but also on membrane characteristics as well. (C) 2000 Elsevier Science S.A. All rights reserved.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000088378500028&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Chemistry, AnalyticalElectrochemistryInstruments & InstrumentationSCI(E)EICPCI-S(ISTP)
Candidate high-resolution mass spectrometry-based reference method for the quantification of procalcitonin in human serum using a characterized recombinant protein as a primary calibrator
No full textInternational audienceProcalcitonin (PCT) is a widely used biomarker for rapid sepsis diagnosis and antibiotic stewardship. Variability of results in commercial assays has highlighted the need for standardization of PCT measurements. An antibody-free candidate reference measurement procedure (RMP) based on the isotope dilution mass spectrometry and protein calibration approach was developed and validated to quantify PCT in human serum. The method allows quantification of PCT from 0.25 to 13.74 μg/L (R > 0.998) with extension up to 132 μg/L after dilution of samples with PCT concentration above 13.74 μg/L. Intraday bias was between −3.3 and +5.7%, and interday bias was between −3.0 and −0.7%. Intraday precision was below 5.1%, and interday precision was below 4.0%. The candidate RMP was successfully applied to the absolute quantification of PCT in five frozen human serum pools. A recombinant PCT used as a primary calibrator was characterized by high-resolution mass spectrometry and amino acid analysis to establish traceability of the results to the SI units. This candidate RMP is fit to assign target values to secondary certified reference materials (CRMs) for further use in external quality assessment schemes to monitor the accuracy and comparability of the commercially available immunoassay results and to confirm the need for improving the harmonization of PCT assays. The candidate RMP will also be used to evaluate whether the correlation between the candidate RMP and immunoassays is sufficiently high. Overall, this candidate RMP will support reliable sepsis diagnosis and guide treatment decisions, patient monitoring, and outcomes
Candidate High-Resolution Mass Spectrometry-Based Reference Method for the Quantification of Procalcitonin in Human Serum Using a Characterized Recombinant Protein as a Primary Calibrator
No full textProcalcitonin
(PCT) is a widely used biomarker for rapid sepsis
diagnosis and antibiotic stewardship. Variability of results in commercial
assays has highlighted the need for standardization of PCT measurements.
An antibody-free candidate reference measurement procedure (RMP) based
on the isotope dilution mass spectrometry and protein calibration
approach was developed and validated to quantify PCT in human serum.
The method allows quantification of PCT from 0.25 to 13.74 μg/L
(R > 0.998) with extension up to 132 μg/L
after
dilution of samples with PCT concentration above 13.74 μg/L.
Intraday bias was between −3.3 and +5.7%, and interday bias
was between −3.0 and −0.7%. Intraday precision was below
5.1%, and interday precision was below 4.0%. The candidate RMP was
successfully applied to the absolute quantification of PCT in five
frozen human serum pools. A recombinant PCT used as a primary calibrator
was characterized by high-resolution mass spectrometry and amino acid
analysis to establish traceability of the results to the SI units.
This candidate RMP is fit to assign target values to secondary certified
reference materials (CRMs) for further use in external quality assessment
schemes to monitor the accuracy and comparability of the commercially
available immunoassay results and to confirm the need for improving
the harmonization of PCT assays. The candidate RMP will also be used
to evaluate whether the correlation between the candidate RMP and
immunoassays is sufficiently high. Overall, this candidate RMP will
support reliable sepsis diagnosis and guide treatment decisions, patient
monitoring, and outcomes
Tuning the Luminescence and Electroluminescence of Diphenylboron Complexes of 5-Substituted 2-(2‘-Pyridyl)indoles
No full textTo examine the effect of substituent groups on the luminescence of BPh2(X-2-PI) complexes,
three new air-stable boron complexes BPh2(F-2-PI) (5a), BPh2(Cl-2-PI) (5b), and BPh2(CH3O-2-PI) (5c) were synthesized and characterized, where F-2-PI = 5-fluoro-2-(2‘-pyridyl)indole,
Cl-2-PI = 5-chloro-2-(2‘-pyridyl)indole, and CH3O-2-PI = 5-methoxyl-2-(2‘-pyridyl)indole. In
these complexes, the 5-substituted 2-PI ligand chelates in a tretrahedral fashion to the boron
center. Compounds 5a−c are luminescent, with 5a having the highest emission efficiency.
Compared with the emission maximum of BPh2(2-PI) (516 nm), the emission maximum of
5a and 5b is blue-shifted to 490 and 487 nm, respectively, while the emission of 5c is red-shifted to 532 nm, indicating the possibility of tuning the luminescence of these complexes
by varying the substituent groups on the 2-PI ligand. An electroluminescent device using
compound 5a as the emitter and the electron transport material has been fabricated
Tuning the Luminescence and Electroluminescence of Diphenylboron Complexes of 5-Substituted 2-(2‘-Pyridyl)indoles
No full textTo examine the effect of substituent groups on the luminescence of BPh2(X-2-PI) complexes,
three new air-stable boron complexes BPh2(F-2-PI) (5a), BPh2(Cl-2-PI) (5b), and BPh2(CH3O-2-PI) (5c) were synthesized and characterized, where F-2-PI = 5-fluoro-2-(2‘-pyridyl)indole,
Cl-2-PI = 5-chloro-2-(2‘-pyridyl)indole, and CH3O-2-PI = 5-methoxyl-2-(2‘-pyridyl)indole. In
these complexes, the 5-substituted 2-PI ligand chelates in a tretrahedral fashion to the boron
center. Compounds 5a−c are luminescent, with 5a having the highest emission efficiency.
Compared with the emission maximum of BPh2(2-PI) (516 nm), the emission maximum of
5a and 5b is blue-shifted to 490 and 487 nm, respectively, while the emission of 5c is red-shifted to 532 nm, indicating the possibility of tuning the luminescence of these complexes
by varying the substituent groups on the 2-PI ligand. An electroluminescent device using
compound 5a as the emitter and the electron transport material has been fabricated
