259,945 research outputs found
LIN-39 does not regulate TRN fate markers in AVM.
No full text(A) AVM was posteriorly displaced in lin-39(n1760) mutants, but the expression of TRN fate marker uIs115[mec-17p::TagRFP] was not affected in lin-39 mutants. (B) The displaced AVM also expressed the TRN fate marker zdIs5[mec-4p::GFP] in lin-39 mutants. Scale Bars = 100 μm. (TIF)</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
LIN-39 motif in the promoters of neuronal fate markers.
No full text(A) The sequences of functional Hox sites identified in this study (Fig 3), which likely mediate the regulation of neuronal fate markers by LIN-39. (B) The sequence logo of LIN-39 binding sites generated from ChIP-seq data. These logos were downloaded from http://cisbp.ccbr.utoronto.ca/. (TIF)</p
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
LIN-5 phosphorylation by cell cycle and polarity kinases <i>in vitro</i>.
No full text(A) Graphic overview of mass spectrometry analysis of in vitro kinase assays, revealing multiple target residues in LIN-5 phosphorylated by PAR-1, PKC-3, CK1, CK2, GSK3, CDK1 and Aurora B kinase. Yellow residues indicate in vivo phosphorylated residues, brackets indicate essential residues identified in complementation assay. (B) Radioactive counts (CPM) of in vitro kinase assays with CK1 and GSK3 on a synthetic LIN-5 peptide 654–670 with or without synthetic incorporation of phosphorylated amino acids S659 or S662.</p
LIN-1 sumoylation is required for ventral toroid contraction.
No full text(A) Wild-type and K10A, K169A mutant LIN-1::GFP expression in L3 larvae at the Pn.px stage after VPC-specific degradation of AID::SMO-1 from the L2 stage onward. The 1° and 2° VPC descendants are underlined in white. The left panels show the corresponding DIC images overlaid with the LIN-1::GFP signal in green. (B) Quantification of LIN-1::GFP expression levels in 1° and 2° VPC descendants at the Pn.px stage in LIN-1::GFP wild-type and K10A, K169A double mutants under the indicated conditions. See S3 Fig for the corresponding measurements at the Pn.pxx stage. (C) Toroid morphogenesis defects in LIN-1 K10A and K169A single and double mutants at the L4 stage. Left panels show lateral views of z-projections. vulA and vulB1 toroids are outlined by the white rectangle in the top left panel and shown in top (xz) views in the right panels. (D) Quantification of vulA contraction, calculated as the ratio of the vulA and vulB1 toroid diameter. The box plots show the median values with the 25th and 75th percentiles and the whiskers indicate the maximum and minimum values. Where indicated, untreated controls are labelled with–IAA (blue) and animals treated with 1 mM auxin with +IAA (red). In each graph, the numbers of animals scored are indicated by the numbers in brackets. Statistical significance in (B) and (D) was calculated with unpaired two-tailed t-tests. p-values are indicated as * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. The scale bars are 10 μm.</p
"Third Generation"-Type Functional Tris(2-pyridyl)borate Ligands and their Transition-Metal Complexes
No full textPhenyltris(2-pyridyl)borates (Tpyb) are a promising class of tripodal “scorpionate”-type ligands with potential utility in the development of transition metal complexes with interesting optical, electronic or magnetic properties, and as building blocks to metallosupramolecular polymers. We report here a new class of “third generation”-type Tpyb ligands that contain different functional groups attached to the boron-bound aryl moiety. The synthesis, characterization and metal ion complexation behavior of ligands with iodo and trimethylsilyl groups is discussed. The electrochemical and absorption characteristics of the corresponding low-spin Fe(II) and Ru(II) complexes are compared. We demonstrate the further elaboration of the iodo derivatives with alkynes via Sonogashira-Hagihara coupling, a process that proceeds with high yield for the Fe(II) and Ru(II) complexes, but not for the free ligand. The borylation of the silyl-substituted Ru(II) complex with BBr3 was also investigated. In addition to the expected borylation product, Ru(Tpyb-Bpin)2, the replacement of one (major product) or two phenyl groups is observed, suggesting that electrophilic borylation occurs at both the C(Ph)-Si and the C(Ph)-B aromatic carbons. The successful attachment of a range of different functional groups at the periphery of the Tpyb metal complexes is expected to provide opportunities to access new polymeric materials via C-C coupling or click-type reactions.Peer reviewe
A single E-box in the <i>Cel-lin-3</i> CRM is not sufficient for <i>lin-3</i> expression in the anchor cell of <i>C</i>. <i>elegans</i>.
No full text(A) New cis-regulatory lin-3 alleles with deleted E-boxL and NHR or NHR and E-boxR. (B) Quantification of vulval induction in these new mutants. Note the complete absence of any induction in the recovered lin-3 alleles (n>30). Scorings of lin-3(1417) animals are the same as those reported in Fig 5 and are used here to indicate that this mutation leads to vulval hypo-induction rather than no induction at all. (C-D) smFISH in lin-3(mf72) (C) and N2 (D) animals. Green spots correspond to lin-3 transcripts and red spots to lag-2 that is used as an anchor cell marker. Blue is DAPI staining of nuclei. Note the absence of lin-3 expression in the anchor cell in the lin-3(mf72) mutant animal. Absence of lin-3 signal in the anchor cell was also confirmed for the other lin-3 alleles.</p
Sinokaratawia Huang & Lin, 2007, gen. nov.
No full textGenus <i>Sinokaratawia</i> gen. nov. <p> <b>Type species</b>. <i>Sinokaratawia prokopi</i> <b>sp. nov.</b></p> <p> <b>Etymology</b>. Named after the Latin name for China and the genus <i>Karatawia</i>.</p> <p> <b>Diagnosis</b>. Wing characters only. Forewing Ax2 strongly oblique; subdiscoidal cell posteriorly open in male but posteriorly closed or nearly so in female; MP straight, MAa with a smooth bend, distally zigzagged, a constricted area between MAa and MP; a very acute projecting anal angle in male hind wing; a distinct constriction of the area between RP 3/4 and IR2; MAa distally zigzagged; no long basal branch of AA parallel to AP; two rows of cells in forewing postdiscoidal area and basal part of area between MP and CuA; CuAa short.</p>Published as part of <i>Huang, - Y. & Lin, - B., 2007, A new genus of isophlebioid damsel-dragonflies (Odonata: Isophlebioptera: Campterophlebiidae) from the Middle Jurassic of China, pp. 13-22 in Zootaxa 1642</i> on page 14, DOI: <a href="http://zenodo.org/record/179672">10.5281/zenodo.179672</a>
Singaporemma banxiaoensis Lin & Li 2014
No full textSingaporemma banxiaoensis Lin & Li, 2014 Figures 6B–b, 7C Singaporemma banxiaoensis Lin & Li, 2014: 42, figs 4–6, 16C–D, 20A Examined material. Holotype ♂, paratypes 1♂ and 1♀ (IZCAS), CHINA: Guangxi, Pingxiang, Xiashi Town, Xinming Village, Banxiaotun, Banxiao Cave, 22°5.542'N, 106°52.148'E, altitude 175 m, 26 July 2011, X. Wang leg. Diagnosis. Male of this species is similar to S. halongense (Fig. 6A) and S. lenachanae (Fig. 6D), but can be distinguished from the latter two by the narrower, pointed embolic tip (Fig. 6b vs. Fig. 6a, 6d), and by the vestigial white eyespots lacking black ocular base in the both sexes (see Lin & Li, 2014: fig. 4G–H vs. Lin et al., 2017: figs 16E–F, 21A). Female is close to S. takensis sp. n. in having a similar configuration of vulva, but differs from the latter by the inverted triangular inner vulval plate, the wider, shorter central process (Fig. 7C vs. Fig. 5C–D). Description. See Lin & Li, 2014: 42. Distribution. China (Guangxi) (Fig. 10).Published as part of Yan, Fanhu & Lin, Yucheng, 2018, A review of the spider genus Singaporemma (Araneae: Tetrablemmidae), with the description of a new species, pp. 329-346 in Zootaxa 4392 (2) on page 331, DOI: 10.11646/zootaxa.4392.2.6, http://zenodo.org/record/119544
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