1,721,001 research outputs found
Overexpression, purification and characteristics of the cellulase gene from Paenibacillus campinasensis BL11
No full text由Paenibacillus campinasensis BL11 之基因中選殖出的基因片段(CelBL11)具有對對羧甲基纖維素（CMC）有活性的內切型葡聚醣酶於大腸桿菌中表達。CelBL11 由1,465 個核苷酸所組成，而此片段的末端並沒有停止密碼子。CelBL11經轉譯後形成約 55 kDa 的內切葡聚醣酶的蛋白質。其N 端包含一個經由預測的29 個氨基酸長度的訊息肽。催化中心(catalytic domain)是被歸類在family 5（GH5）之中。在纖維素酶的C 端有碳水化合物結合區是被歸類在在family III（CBM3）之中。重組蛋白在纖維素酶的N 端上有His-tag 的片段是用來作NTA-Agarose 親合性管柱曾析純化蛋白質所用。在SDS-PAGE 顯示分別在52 kD 和38 kDa 的位置上有內切型纖維素酶之活性。由核甘酸所推測出的長度大概與38 kDa 相差14kDa 左右。有可能是因為後修飾作用或是蛋白質酶水解所導致的結果。38 kDa纖維素酶對avicel, acid swollen avicel, CMC, 1,3B-glucan 和xylan 可作為分解的基質。經由純化後的38 kDa 纖維素酶其最適酸鹼條件為pH 7 最適溫度為60 oC。Hg2+和 N-bromosuccinimide 對纖維素酶具有劇烈的抑制；而Mn2+對纖維素酶有促進的效果。當環境pH 6 和7 時，在60 oC 的環境下八個小時後活性只減少的百分之二十以下；pH 5 和8 其相對活性分別為31％和15％。纖維素酶酵素動力學常數在基質為羧甲基纖維素時Km 和Vmax 分別為11.25 mg/ml 與1250mol/min/mg。當環境cellobiose 的濃度在5 mg/ml 以下時，基質與cellobiose 為競爭型抑制。From Paenibacillus campinasensis BL11, the gene CelBL11 encoding an
endoglucanase with an activity towards carboxymethyl cellulose (CMC) was cloned
and expressed in E. coli. CelBL11 is composed of 1,465 bp nucleotides without stop
coden in C-terminal. Cel-BL11 encodes an endoglucanase of 55 kDa. The N-terminal
of the cellulase contains a deduced signal peptide of 29 amino acids in length and a glycosyl hydrolase domain (catalytic domain) in family 5 (GH5); and a carbohydrate binding module in family III (CBM3) at C-terminal of this cellulase. The Cel-BL11 was fused with a His-tag at its N-terminal and the recombinant Cel-BL11 was purified by Ni-NTA affinity chromatography. Zymographic analysis of the recombinant Cel-BL11 exhibited two cellulase activities at 52 kDa and 38 kDa. Apparent ca. 14 kDa difference between the recombinant 38 kDa cellulase and its precursor form minus the signal peptide (ca. 3 kDa) could be due to proteolysis or post-translational modifications. The 38 kDa cellulase hydrolyzed avicel, acid swollen avicel, CMC, 1,3B-glucan and xylan. Optimum temperature and pH for the 38 kDa cellulase activity of the purified cellulase were found to be 60 °C and pH 7.0, respectively. The 38 kDa cellulase activity was strongly inhibited by Hg2+ and N-bromosuccinimide, andstrongly promoted by Mn2+. More than 80% residual activities at 60 oC for 8 h were demonstrated at pH 6 and 7; while 31 and 15% residual activities found for pH 5 and 8. The 38 kDa cellulase has a Km of 11.25 mg/ml and a Vmax of 1250 mol/min/mg with carboxymethyl-cellulose (CMC). Competitive inhibition of cellobiose below 5 mg/ml on CMC hydrolysis by Cel-BL11 was found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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