67,939 research outputs found
Suramin Inhibits the Toxic Effects of Presynaptic Neurotoxins at the Mouse Motor Nerve Terminals
No full textClinically available chemical antagonists of snake neurotoxins still await to be identified. In this study, we demonstrate that an anti- trypanosomiasis agent, suramin, is an effective inhibitor of -bungarotoxin isolated from the venom of Formosan Krait snake. Following intraperitoneal injection (12 ng/g) of -bungarotoxin in mice, the time to paralysis (loss a limb withdrawal reflex, 21.8±3.4 h, n=4) was significantly prolonged after intravenous injection (16 g/g) of suramin ( 35.9±4.0 h, n=4, P<0.05). The mechanism of this inhibitory effect of suramin was analyzed at the mouse nerve terminals. -Bungarotoxin (1 g/ml) produces an irreversible blocking effect of nerve-evoked muscle contractions of mouse phrenic nerve–diaphragm (blocking time 135±6 min, n=6). Pretreatment with suramin (0.3 mM) significantly prolonged the blocking time by three-fold. This selective inhibitory effect of suramin was further confirmed when suramin was shown to delay the neuromuscular blocking effect of another presynaptic neurotoxin, crotoxin (from American rattlesnake venom), but not that of the postsynaptic neurotoxin, - bungarotoxin. Furthermore, suramin inhibited -bungarotoxin in blocking transmitter release as revealed by prolonging the time to abolish the end- plate potential amplitude (with suramin, 391±8 min; without treatment, 141±5 min). K+ current was measured in the mouse triangularis sterni preparation; suramin (0.3 mM) had no significant effect on -bungarotoxin in inhibiting K+ current (77±3% of control; with suramin 75±3% of control, respectively). These findings clearly show that suramin is an inhibitor of presynaptic neurotoxins, mediated by interrupting the toxins in blocking the releasing mechanism of transmitter at the motor nerve terminals. The implication of these findings is that suramin and related compounds can become useful agents in management of snakebites
Keynote: Jon Gertner
No full textThe symposium will start on the evening of April 16 with a keynote address by Jon Gertner. Jon is a journalist, historian, and feature writer for The New York Times Magazine
as well as the author of the NYTimes bestseller, The Idea Factory. His address will focus on the issue of intellectual property and the ethical questions around the huge amount of human-generated content that large language models use as they are developed
Enhanced Spontaneous Transmitter Release at Murine Motor Nerve Terminals with Cyclosporine
No full textCyclosporine, a calcineurin inhibitor, significantly enhances spontaneous acetylcholine release after a brief tetanus and potentiates the effect of phorbol 12,13- dibutyrate. Both actions are prevented by the protein kinase C inhibitor, bisindolylmaleimide iodide. Protein kinase C and calcineurin thus play important roles in the balance between phosphorylation and dephosphorylation regulating spontaneous transmitter release at motor nerve terminals
Jon Mirande eta ironia
No full textLa ironía es un elemento que ha ido siempre unido a la poesía, y especialmente a la poesía moderna.Tras un pequeño repaso a esta en diferentes épocas, se pasa a describir las tres diferentes ironías de Jon Mirande: la intelectual, la social y la filosófica. Todo ello acompañado de ejemplosIrony is an element that has always been united to poetry, and especially to modern poetry. After a small revision of irony in different eras, the author then describes the three different ironies of Jon Mirande: intellectual, social and philosophical irony. All this illustrated with example
Jon Pineda, 32nd Annual ODU Literary Festival
No full textJon Pineda is the author of The Translator\u27s Diary, winner of the Green Rose Prize for Poetry, and BIrthmark, winner of the Crab Orchard Award Series in Poetry Open Competition. His memoir, Sleep in Me, is forthcoming in 2010 from the University of Nebraska Press. He teaches in the low-residency MFA program at Queens University of Charlotte
Min Lin et Maria Galikowski, The Search for Modernity
No full textBéja Jean-Philippe. Min Lin et Maria Galikowski, The Search for Modernity. In: Perspectives chinoises, n°56, 1999. pp. 72-74
LIN-2 and FRM-3 are required to maintain locomotory behaviour.
No full text(A) Genomic structure of lin-2 and frm-3 locus, mutant allele characterization, and protein architecture for LIN-2 and FRM-3. The whole promoter region of lin-2a and part of the kinase domain of LIN-2A is deleted in the e1309 mutants. The FERM domain in both frm-3a and frm-3b is deleted in the gk585 mutants. Syb1019 and syb1036 are stop codons in lin-2 and frm-3 that inactivate the expression of lin-2a and lin-2b, and frm-3a and frm-3b. (E, F) Locomotion speed is reduced by the loss of LIN-2 and FRM-3. Representative trajectories of locomotion in wild type (E) and mean locomotion speed in wild type, lin-2(e1309), frm-3(gk585), lin-2(syb1019), frm-3(syb1036), and lin-2(e1309);frm-3(gk585) mutants. To measure locomotion speed, young adult animals were washed with a drop of PBS and then transferred to fresh NGM plates with no bacterial lawn (30 worms per plate). Worm movement recordings (under room temperature 22°C) were started 10 min after the worms were transferred. A 2 min digital video of each plate was captured at 3.75 Hz frame rate by WormLab System (MBF Bioscience). Average speed and tracks were generated for each animal using WormLab software. To confirm the repeatability of the data, the locomotion speed was measured in two independent experiments in two days. For each mutant, around 10–40 animals were analyzed in one experiment. Significance was tested for each experiment. Data are mean ± SEM (**, p p < 0.001 when compared to wild type; n.s., non-significant; one-way ANOVA). The number of worms analyzed for each genotype is indicated in the bar.</p
Interview with Jon Baskin--May 15, 2015
No full textJon Baskin is co-founder and editor of The Point magazine in Chicago. He is also a graduate student at the University of Chicago's Committee on Social Thought and the author of many essays and works of criticism for venues such as The Los Angeles Review of Books, The Nation, n+1, The New York Observer, BookForum, Salon, and The Point. Earlier in his career he was a fact checker for various magazines, including Popular Science, Inc Magazine, The Atlantic Monthly, and n+1. The interview was conducted at the office of The Point in Chicago on May 15, 2015.1_izzia9z
Jon Sands, 41st Annual ODU Literary Festival
No full textJon Sands is the author of The New Clean (2011), as well as the co-host of The Poetry Gods podcast. His work has been published widely, and anthologized in The Best American Poetry. He’s a youth mentor with Urban Word-NYC, and teaches creative writing for adults at Bailey House in East Harlem (an HIV/AIDS service center). He’s a recent MFA graduate in fiction from Brooklyn College, where his work won the Himan Brown Award for short stories, and he has represented New York City multiple times at the National Poetry Slam. He lives in Brookly
Min hua tai yang neng dian chi dian ji cai liao zhi yan fa
No full textLee, Tien Lin Lawrence = 敏化太陽能電池電極材料之研發 / 李天麟.Thesis M.Phil. Chinese University of Hong Kong 2014.Includes bibliographical references.Abstracts also in Chinese.Title from PDF title page (viewed on 28, October, 2016).Lee, Tien Lin Lawrence = Min hua tai yang neng dian chi dian ji cai liao zhi yan fa / Li Tianlin
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