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    Mitochondrial genomic comparison provides evolutionary insights into relationships between azooxanthellate and zooxanthellate scleractinians (Cnidaria; Anthozoa)

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    近年來利用分子親緣研究顯示無共生藻石珊瑚的演化關係仍需進一步探討。 本論文以Fungiacyathus stephanus和Madrepora oculata兩種無共生藻石珊瑚,以及Polycyathus sp.和Euphyllia ancora兩種有共生藻石珊瑚作為研究材料,比較無共生藻石珊瑚和有共生藻石珊瑚粒線體基因組中的差異,並和已知石珊瑚粒線體基因比較,加以分析其親緣關係。 研究結果發現,無共生藻石珊瑚粒線體基因組和有共生藻石珊瑚基因組相似,但個別支序中擁有許多獨立的特徵: (一)本研究首度發現珊瑚粒線體基因組內基因間的排列重組。在Madrepora粒線基因組中,細胞色素氧化酶第二次單元基因 (cytochrome oxidase subunit II, cox2) 和細胞色素氧化酶第三次單元基因 (cytochrome oxidases subunit III, cox3) 之間發生基因重新排列。另外,NADH脫氫酶第四次單元基因 (NADH dehydrogenase subunit 4, nad4) 的五撇端具有胸腺嘧啶的細胞間異質性現象 (heteroplasm) ; (二) Fungiacyathus具有較長的基因間區且在細胞色素氧化酶第一次單元基因 (cytochrome oxidase subunit I, cox1) 中發現第一型介入子 (group I intron) 插入,而造成其基因體結構較為鬆散 (> 19 kb);(三)相反的,因基因間區較短,使得Polycyathus則為目前已知珊瑚粒線體中基因排列最緊密的粒線體 (< 16 kb);(四)在Euphyllia中發現目前石珊瑚中已知最長的cox1基因。 以粒線體基因片段序列親緣關係分析也支持這四個粒線體基因組的特徵演化。Madrepora在石珊瑚中呈現獨立分支,對應了在其基因重組的特殊性;而具有第一型介入子的Fungiacyathus和一群也具有第一型介入子的複雜珊瑚群 (complex clade)有較相近的關係,顯示無共生藻石珊瑚和有共生藻石珊瑚可能具有相同的第一型介入子來源;而Polycyathus和Euphyllia的分據親緣樹上的強壯珊瑚系群 (robust clade) 和複雜珊瑚系群,則支持了葵珊瑚亞目 (suborder Caryophylliina) 為非單一起源。Because only a few studies of azooxanthellate scleractinians exist and results of 16S rDNA analyses of coral phylogeny are ambiguous, a mitochondrial genomics study can provide further understanding of scleractinian evolution. Both Euphyllia ancora and Polycyathus sp. which contain zooxanthellae belong to the suborder Caryophylliina and were previously separated into the robust subclade with family-level debates. The genera, Fungiacyathus and Madrepora, have also been the subject of taxonomic debate after radioimmunoassay and mitochondrial sequence analyses.our mitochondrial (mt) genomes, two from azooxanthallate corals, Fungiacyathus stephanus and Madrepora oculata, and two from zooxanthellate corals, Euphyllia ancora and Polycyathus sp., were sequenced in this study. Three major features were discovered in the azooxanthellate mt genomes. First, gene rearrangement of cytochrome oxidase subunit II (COII) and cytochrome oxidase subunit III (COIII) and a poly (T) variation in the nad4 protein-coding gene were found in the mt genome of M. oculata. These characters are discussed in the Scleractinia for the first time. Second, F. stephanus presented a loose genome (19,381 bp) with a group I intron in the cox1 gene. In contrast, the zooxanthellate coral, Polycyathus sp., had the most compact mt genome (15,356 bp) among all the scleractinians known to date. Euphyllia ancora presented an extremely long cox1 gene. Phylogenetic analyses also showed unique information of these four mt genomes in the evolutionary trees, and M. oculata formed a single lineage with robust clade corals, supporting the result of rearrangement of the COII and COIII genes. Fungiacyathus stephanus grouped with the Poritidae. The cox1 group I intron occurs in the Fungiacyathus-Poritidae lineage. The high sequence similarity of cox1 intron nucleotides between Fungicyathus and the Poritidae indicates that azooxanthellate and zooxanthellate corals have the same origin of cox1 group I intron. The two Caryophylliina corals, Polycyathus and Euphyllia, were respectively grouped with the robust and complex clades. This suggests that Caryophylliina corals are not monophyletic. In conclusion, based on a mitogenomic approach, this study provides some insights into the phylogenetic relationships and evolution of mitochondrial genomes among these taxonomically ambiguous taxa.口試委員會審定書…………………………………………………………………i謝……………………………………………………………………………………ii文摘要………………………………………………………………………………………… …2bstract…………………………………………………………………………………………3ntroduction…………………………………………………………………………………………5eneral biology of azooxanthellate scleractinian corals……………………………………………5he evolutionary history of azooxanthellate corals……………………………………………………6olecular phylogenetics of scleractinians and the phylogenetic position of azooxanthellate corals……………………………………………………………………………………………………8itogenomic approaches to azooxanthellate corals……………………………………………………9ims of this study………………………………………………………………………………………11aterials and Methods………………………………………………………………………………13oral sample collection………………………………………………………………………………13oral species…………………………………………………………………………………………13NA extraction………………………………………………………………………………………16ong-PCR and universal primer design………………………………………………………………17loning and sequencing………………………………………………………………………………18enome annotation and sequence analysis……………………………………………………………19mplification of poly (T) variation within the nd4 gene in M oculata……………………………20olecular phylogeny…………………………………………………………………………………21esult…………………………………………………………………………………………………22itochondrial genome organization…………………………………………………………………22ene rearrangement and the variation of poly thymine of nad4 in M oculata………………………23ntergenic spaces and gene density…………………………………………………………………24ene length……………………………………………………………………………………………25ucleotide composition………………………………………………………………………………25RNA structure………………………………………………………………………………………26odon usage…………………………………………………………………………………………26hylogeny………………………………………………………………………………………………28iscussion……………………………………………………………………………………………30nique mitochondrial genome of M oculata………………………………………………………30ungiacyathus`s cox1 group I intron and its phylogenetic relationship with Corallimorparia………33ompact genome of Polycyathus sp…………………………………………………………………35hylogenetic analysis of Polycyathus and Euphyllia support the suborder caryophylliina is not monophyletic……………………………………………………………………………………………36onclusion……………………………………………………………………………………………39eference………………………………………………………………………………………………4

    Society of Composers Region VI Conference - Concert 2 Thursday. February 9. 2006 8:00 p.m. Lillian H. Duncan Recital Hall

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    Program: Falling through Crimson and Lead / Suzanne Sorkin -- Madison Sketchbook / John G. Bilotta -- Nocturne No.3 / Francisco Castillo Trigueros -- Schematic Nocturne / Bruce Bennett -- Turning / Derek Bermel -- Once It Was / Trent Hanna -- Interaction / Mei-Fang Lin -- Dialogue for two pianos / Florie Namir.No program is available for this performance

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Sounds of a single-family house

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    The purpose of this piece, “Sounds of a Single-Family House,” is to study how sounds change due to the varying reverberation times of different spaces. Different frequencies stimulate different impulse responses within the same space, changing how certain sounds are heard. This piece primarily uses sounds recorded from a two-story, single-family house with a basement as its musical materials. Some of these sounds went through further audio signal processes to make the music more interesting. Ultimately, the piece explores the musical possibilities that exist within the sounds of daily life, with an aim to extend or even redefine what music can be.Embargo status: Restricted to TTU community only. To view, login with your eRaider (top right). Others may request access exception by clicking on the PDF link to the left

    Concertino for Alto Saxophone with Oboe, Horn in F, and Piano

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    Concertino for Alto Saxophone with Oboe, Horn in F, and Piano, a three-movement small-form concerto, provides performers with an opportunity to present music with atonal melodic and harmonic concepts composed in a method that is quite tonal. It is written with the intention of presenting atonality in such a way that it is "pleasing to the ears." Through the use of fragmentation, repetition, and motivic expansion, atonal concepts in this work develop in much the same way as melodies of the Common Practice period. Through the juxtaposition with and the interjection of tonal textures, Concertino advances a forced bond between tonality and atonality

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    This man receives sinners, a cantata on Luke 15

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    This Man Receives Sinners, a cantata for mixed voices and chamber ensemble, is a setting of the full text of the 15th Chapter of the Gospel of Luke. This text contains three parables of Jesus that concern the lost and the found. The parables are told in response to the complaint of the Pharisees and scribes in Luke 15:2 that Jesus welcomed sinners and ate with them. The three parables are those of a shepherd with a lost sheep, a woman with a lost coin, and a man with two sons. This composition is through composed and consists of five major sections with no formal breaks between them. Three sections setting the three parables of Jesus in Luke 15 follow the introductory section. The work concludes with a short section reprising material from the four sections prior. While grounded in extended tertian musical language, the melodic and harmonic language of the work spans modal, tonal, and atonal domains. Formally, it contains vocal solos in arioso style, choruses voiced by full and partial choirs, as well as passages of purely instrumental music. The choice and placement of these musical elements within the piece are determined by the structure of the source text. Indeed, it is the source text that drives every aspect of the work: melody, harmony, and form

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods
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