257,040 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
A comment on "Intergenerational equity: sup, inf, lim sup, and lim inf"
No full textWe reexamine the analysis of Chambers (Social Choice and Welfare, 2009), that produces a characterization of a family of social welfare functions in the context of intergenerational equity: namely, those that coincide with either the sup, inf, lim sup, or lim inf rule. Reinforcement, ordinal covariance, and monotonicity jointly identify such class of rules. We show that the addition of a suitable axiom to this three properties permits to characterize each particular rule. A discussion of the respective distinctive properties is provided.Social welfare function; Intergenerational equity; Lim sup ; Lim inf
Interpretare la LIM
No full textIl capitolo presenta le forme di utilizzo della LIM secondo la prospettiva delle competenze digitali
Strumenti di valutazione e progettazione delle attività con la LIM
No full textIl capitolo presenta le nuove forme di comunicazione introdotte dalla LIM e le organizzazioni didattiche innovative relative a progettazione e valutazione
Data used for Lim et al. (2024)
No full text<p>Data used in Lim et al. (2024)</p>
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<p>Lim, H., Cho, C. S., & Son, M. (2024). The 2022 Goesan earthquake of the moment magnitude 3.8 along the buried fault in the central Korean Peninsula. Journal of Seismology. https://doi.org/10.1007/s10950-024-10201-y</p>
Lim (Or Lim You), Mr H P, [No Service Number]
No full textThis record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/399461Surname: LIM (OR LIM YOU). Given Name(s) or Initials: MR H P. Military Service Number or Last Known Location: [No Registration Number]. Missing, Wounded and Prisoner of War Enquiry Card Index Number: 47767.217246
Item: [2016.0049.31754] "Lim (Or Lim You), Mr H P, [No Service Number]
Four and a half LIM protein 1C (FHL1C)
No full textFour-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C
Specificity of single LIM motifs in targeting and LIM/LIM interactions in situ
No full textThe LIM motif defines a double zinc finger structure found in proteins involved in cell fate determination and growth control. LIM proteins, which include LIM homeo domain, LIM kinase, focal adhesion, and LIM-only proteins, usually contain two or more LIM motifs clustered at their amino- or carboxy-terminal end. At present, the mode of action of the LIM domain is not clear. In this study we have analyzed the binding properties of LIM motifs in the cellular environment. We show that MLP, CRP, and betaCRP define a subclass of LIM-only proteins with unique dual subcellular localization in the nucleus and along actin-based filaments in the cytosol. A double MLP construct that accumulated nearly exclusively along actin filaments promoted myogenic differentiation efficiently, arguing for a functional role of cytoskeleton-associated MLP. Binding of MLP to the actin cytoskeleton is specifically attributable to its second LIM motif. An additional LIM motif potentiates binding. Potentiating LIM motifs can be interchanged, resulting in differential targeting of interacting proteins. To analyze LIM-LIM interactions in situ, this property was exploited to develop a hybrid interaction approach based on the relocalization of LIM-containing constructs to the actin cytoskeleton. These experiments revealed the existence of marked selectivity in the interactions of single LIM motifs, and among LIM domains from different LIM-homeo domain and LIM-only proteins. Furthermore, the analysis suggested that the LIM motif has two interacting interfaces. On the basis of these findings, we propose that LIM motifs function as specific adapter elements to promote the assembly and targeting of multiprotein complexes
Strumenti di valutazione e progettazione delle attività con la LIM
No full textIl capitolo presenta le nuove forme di comunicazione introdotte dalla LIM e le organizzazioni didattiche innovative relative a progettazione e valutazione
Bluff bodies in deep turbulent boundary layers: Reynolds-number issues
No full textIt is generally assumed that flows around wall-mounted sharp-edged bluff bodies
submerged in thick turbulent boundary layers are essentially independent of the
Reynolds number Re, provided that this exceeds some (2–3) × 104. (Re is based on
the body height and upstream velocity at that height.) This is a particularization of
the general principle of Reynolds-number similarity and it has important implications,
most notably that it allows model scale testing in wind tunnels of, for example,
atmospheric flows around buildings. A significant part of the literature on wind
engineering thus describes work which implicitly rests on the validity of this
assumption. This paper presents new wind-tunnel data obtained in the ‘classical’ case
of thick fully turbulent boundary-layer flow over a surface-mounted cube, covering an
Re range of well over an order of magnitude (that is, a factor of 22). The results are
also compared with new field data, providing a further order of magnitude increase in
Re. It is demonstrated that if on the one hand the flow around the obstacle does not
contain strong concentrated-vortex motions (like the delta-wing-type motions present
for a cube oriented at 45? to the oncoming flow), Re effects only appear on fluctuating
quantities such as the r.m.s. fluctuating surface pressures. If, on the other hand, the
flow is characterized by the presence of such vortex motions, Re effects are significant
even on mean-flow quantities such as the mean surface pressures or the mean velocities
near the surfaces. It is thus concluded that although, in certain circumstances and for
some quantities, the Reynolds-number-independency assumption is valid, there are
other important quantities and circumstances for which it is not
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