30,726 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Four and a half LIM protein 1C (FHL1C)
Get PDFFour-and-a-half LIM domain protein 1 isoform A (FHL1A) is predominantly expressed in skeletal and cardiac muscle. Mutations in the FHL1 gene are causative for several types of hereditary myopathies including X-linked myopathy with postural muscle atrophy (XMPMA). We here studied myoblasts from XMPMA patients. We found that functional FHL1A protein is completely absent in patient myoblasts. In parallel, expression of FHL1C is either unaffected or increased. Furthermore, a decreased proliferation rate of XMPMA myoblasts compared to controls was observed but an increased number of XMPMA myoblasts was found in the G(0)/G(1) phase. Furthermore, low expression of K(v1.5), a voltage-gated potassium channel known to alter myoblast proliferation during the G(1) phase and to control repolarization of action potential, was detected. In order to substantiate a possible relation between K(v1.5) and FHL1C, a pull-down assay was performed. A physical and direct interaction of both proteins was observed in vitro. In addition, confocal microscopy revealed substantial colocalization of FHL1C and K(v1.5) within atrial cells, supporting a possible interaction between both proteins in vivo. Two-electrode voltage clamp experiments demonstrated that coexpression of K(v1.5) with FHL1C in Xenopus laevis oocytes markedly reduced K(+) currents when compared to oocytes expressing K(v1.5) only. We here present the first evidence on a biological relevance of FHL1C
Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and Lasp-1
No full textZyxin is a versatile component of focal adhesions in eukaryotic cells. Here we describe a novel binding partner of zyxin, which we have named LIM-nebulette. LIM-nebulette is an alternative splice variant of the sarcomeric protein nebulette, which, in contrast to nebulette, is expressed in non-muscle cells. It displays a modular structure with an N-terminal LIM domain, three nebulin-like repeats, and a C-terminal SH3 domain and shows high similarity to another cytoskeletal protein, Lasp-1 (LIM and SH3 protein-1). Co-precipitation studies and results obtained with the two-hybrid system demonstrate that LIM-nebulette and Lasp-1 interact specifically with zyxin. Moreover, the SH3 domain from LIM-nebulette is both necessary and sufficient for zyxin binding. The SH3 domains from Lasp-1 and nebulin can also interact with zyxin, but the SH3 domains from more distantly related proteins such as vinexin and sorting nexin 9 do not. On the other hand, the binding site in zyxin is situated at the extreme N terminus as shown by site-directed mutagenesis. LIM-nebulette and Lasp-1 use the same linear binding motif. This motif shows some similarity to a class II binding site but does not contain the classical PXXP sequence. LIM-nebulette reveals a subcellular distribution at focal adhesions similar to Lasp-1. Thus, LIM-nebulette, Lasp-1, and zyxin may play an important role in the organization of focal adhesions
Do insectivorous bird communities decline on land-bridge forest islands in Peninsular Malaysia?
No full textTo assess the impact of habitat fragmentation on tropical avian communities, we sampled lowland forest birds on six land-bridge islands and two mainland forest sites in Lake Kenyir, Peninsular Malaysia using timed point counts, hypothesizing that insectivorous birds are the worst affected guild. We used an information-theoretic approach to evaluate the effects of area, isolation, primary dietary guild (omnivore, frugivore and insectivore) and their interactions in predicting species richness, abundance and diversity. Our analysis showed that a model that considered the effects of area, dietary guild and their interaction best explained observed patterns of species richness. But amodel considering both area and dietary guild best explained the variation in abundance. Notably, insectivorous birds were singled out as the dietary guild most sensitive to fragmentation, followed by frugivorous and omnivorous birds and hence provide support for our hypothesis. Assemblages of insectivorous birds were clearly depauperate on anthropogenic forest islands in Lake Kenyir and are consistent with forest fragmentation studies in the Neotropics. Given their specialized foraging ecology and diversity, conservation of intact communities of insectivorous bird guilds in Malaysia will be critical for maintaining predator–prey interactions in lowland tropical forests.Ding Li Yong, Lan Qie, Navjot S. Sodhi, Lian Pin Koh, Kelvin S.-H. Peh, Tien Ming Lee, Haw Chuan Lim and Susan L.-H. Li
DESA1002 'Continuous City' Li Vern Lim
No full textMy building is an Otaku Centre called “オタク天国” which literally means Otaku Heaven. “Otaku” is a Japanese term used to refer to people with obsessive interests, particularly anime, manga and video games. My design caters to the special interests of the mild and also the extreme otaku. The building has anime/manga merchandise stores, cosplay cafes, figure exhibition spaces and a real-life battle simulation maze. The top floor consists of working spaces for anime and game producers. The top floor is a working area. Thus, it was made to look more mechanical. Vertical slits were used as windows, making it look slightly resemble robot (mecha) parts. The ground floor design was meant to look fun and inviting. The design was inspired by the fact that anime is a type of film industry. The walls were made to look like film wrapping around the columns. The film reel acts as the entrance to symbolize the beginning of a journey once one steps through the “reel”. The underground floor was meant for the more extreme otaku. It was designed to look like a huge underground cavern found in many sci-fi anime. It would make people feel as if they have been transported into the anime dimension. Circulation; basically, there are 2 ways to get to each floor; 1) Lifts – for the disabled 2) Stairs – in case the lifts are not working There are 2 entrance points into the building. Workers would enter from the front entrance, which is facing the main road, and go straight up using the lift or stairs. Milder otaku would also enter through the front entrance. The more extreme otaku would choose to go through an alleyway behind the building and enter a lift which would bring them down right into the underground floor where all the hardcore stuff is happening. There is a lift on the ground floor and a ramp along the curvy walls that lead right into the exhibition space
BDES2020 - Decorated Shed <Li Vern, Lim>
No full textANTHILL: where spaces are designed based on paths of circulation. There are different ways in which people move through space; past spaces, through spaces, and into spaces. There are a few layers in the building. The top layer consist of a laser-cut aluminium and plastic composite. Each preferation has a meaning as it responds to the program within. The second floor comprises of working areas with interesting balcony spaces while the ground floor comprises of 2 parts; a curvy and fun area for people to hang out and relax, and a more serious work area. Both floors are connected by vertical circulation; a winding stairway leading up to a platform and a lift for disabled access
Yves-Heng Lim
No full textYves-Heng Lim est enseignant-chercheur au Département d’Etudes de Sécurité et de Criminologie de l’Université Macquarie, Sydney. Il est l’auteur de China’s Naval Power: An Offensive Realist Approach (Ashgate, 2014). Yves-Heng Lim is a lecturer at the Department of Security Studies and Criminology, Macquarie University. He is the author of China’s Naval Power: An Offensive Realist Approach (Ashgate, 2014)
Rational design of Li<sub>3</sub>VO<sub>4</sub>@carbon core–shell nanoparticles as Li-ion hybrid supercapacitor anode materials
No full textA Li-ion hybrid supercapacitor (Li-HSC) delivering high energy within seconds (excellent rate performance) with stable cycle life is one of the most highly attractive energy storage devices.</p
- …
