1,721,357 research outputs found
Kinetics of in vivo inhibition of tissue cathepsin d by pepstatin A
No full text1. 1. We have investigated the kinetics of inhibition of cathepsin D in heart, liver and skeletal muscle of CD-1 mice following administration of 25, 50, 100 and 200 mg/kg i.p. of pepstatin A, a specific inhibitor of this protease. 2. 2. In the liver, a significant inhibition of cathepsin D occurred up to at least 15 days, whereas, in heart and skeletal muscle, this inhibition lasted for a much shorter period of time. 3. 3. These results show that the recovery of enzyme activity to normal values is dose-dependent and that, at the same dose level, marked differences occur in the recovery of enzyme activity in these organ tissues, the liver being the most sensitive one. © 1988
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Pepstatins: Aspartic proteinase inhibitors having potential therapeutic applications
No full textCathepsin D (EC 3.4.23.5) is a lysomal aspartie proteinase that is involved, under normal phusiologycal conditions, ..
Membrane gangliosides and immuno-mediated cytolysis in drug sensitive and treatment-induced multidrug resistant human ovarian cancer cells
No full textThe pattern of cytoplasmic membrane gangliosides and two cellular features which have been reported to be related to the expression of different membrane gangliosides namely adhesion to solid substrates and susceptibility to the lytic activity of immune effector cells have been investigated in drug sensitive A2780 human ovarian cancer cells and in two treatment-induced multidrug resistant sublines (A2780 - DX1 and A2780 - DX3) The total membrane gangliosides content of A2780 sensitive cells was comparable to that of the two multidrug resistant (MDR) sublines but the acquisition of the MDR phenotype was characterized by an increased expression of the polysialylated gangliosides (particularly the disialoganglioside GD1) and decreased expression of the monosialoganglioside GM2. The kinetics of cellular adhesion both to plastic culture dishes and to extracellular matrix coated dishes were similar in the three cell lines indicating that the gangliosides profile seems not to be relevant for cell adhesivity to the above mentioned substrates. When human peripheral blood lymphocytes in toto (PBL) and two lymphokine activated (LAK) T cell subpopulations (CD3+4-8- and CD3-16+) were used as effector cells against A2780 (sensitive e) and A2780-DX3 (highly resistant) cells cytolysis of target cells was more efficient against the A2780-DX3 subline suggesting a possible role of the ganglioside GD1a as a target structure for LAK immunotherapy
Trattamento dell'incontinenza urinaria post-prostatectomia di grado severo mediante l'uso di sling tipo "AdVance"
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Effects of E-64 (cysteine-proteinase inhibitor) and Pepstatin (aspartyl-proteinase inhibitor) on metastasis formation in mice with mammary and ovarian tumors
No full textThe effects of E-64 (Cathespin B and L inhibitor) and Pepstatin A (Cathepsin D inhibitor) on spontaneous and experimental metastasis formation were investigated in mice with MCa mammary carcinoma, M5076 ovarian sarcoma and L1210 leukemia. Pepstatin induced a marked decrease in the number of spontaneous metastasis in MCa or M5076 tumor bearing mice. This phenomenon was also noted with E-64 but only in M5076 tumor bearing mice. On the other hand, both these agents were unable to prevent the formation of experimental metastasis in mice injected i.v. with L1210, MCa or M5076 tumor cells or with tumor cells in which Cathepsin B, E and D activities were inhibited by a 24 hour continuous exposure to high non-cytotoxic concentrations of L-64 and/or Pepstatin. These data suggest that Cathepsin B, E and D seem to be involved in the early steps of the metastatic process rather than in the hematogenous spread of tumor cells. However, other pharmacological activities which may account for the discrepant effects of E-64 or Pepsatin on experimental and spontaneous metastasis cannot be ruled out
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