3,195 research outputs found
Letter from Stephen T. Mather, U.S. National Park Service to Jesse L. Boyce
No full textLetter from the Director of the National Park Service, Stephen T. Mather, to Jesse L. Boyce informing him that immediate action is being taken to remove the TNT from the Grand Canyon
Stephen L. Carter on Civility
No full textStephen L. Carter is one of America’s leading public intellectuals. Throughout his career, Professor Carter has helped to shape the national debate on issues ranging from the role of religion in our politics and culture to the role of integrity and civility in our daily lives. He is the William Nelson Cromwell Professor of Law at Yale University. He served as a law clerk for two of the great veterans of the civil rights movement, including Justice Thurgood Marshall of the U.S. Supreme Court. A member of the American Academy of Arts and Sciences and the American Law Institute, he holds seven honorary degrees. He has published five novels and is the author of seven critically acclaimed nonfiction books on law, ethics and politics, including this year’s FYS common book, Civility: Manners, Morals, and the Etiquette of Democracy
Abstract 3509: EWS/FLI regulates transcriptional activation via length-dependent GGAA microsatellites
No full textAbstract
Objective: The purpose of this study is to investigate how EWS/FLI transcriptionally activates gene targets via polymorphic GGAA microsatellites. Ewing Sarcoma is a pediatric bone malignancy initiated by a t(11;22) chromosomal translocation that produces the EWS/FLI oncoprotein. EWS/FLI transcriptionally activates and represses its target genes to mediate oncogenic reprogramming. Expression of its up-regulated targets correlates with EWS/FLI binding to associated GGAA microsatellites, which show length polymorphisms. These microsatellite polymorphisms may critically affect EWS/FLI-responsiveness of key gene targets. For example, NR0B1 is necessary for EWS/FLI mediated oncogenic transformation, and we found a “sweet-spot” of 20-25 repeat length as optimal for EWS/FLI mediated transcriptional activity at NR0B1 through clinical observation and in vitro studies. The mechanism underlying this optimal length is unknown.
Methods: We explored the stoichiometry and binding affinity of EWS/FLI for different repeat lengths through biochemical studies, including fluorescence polarization, and immunoprecipitation assays, combined with bioinformatics analysis. Additionally, use of EWS/FLI mutant constructs has been critical for elucidating particular binding behavior of EWS/FLI at different microsatellite repeat lengths.
Results: Fluorescence anisotropy studies demonstrate that FLI binding affinity is independent of GGAA microsatellite length. In contrast, the stoichiometry of protein to DNA binding increases in specific incremental patterns with increasing microsatellite repeats. EWS/FLI mutants inform on binding, suggesting a complex relationship between microsatellite length and transcriptional activity.
Conclusion: Overall our data suggests a model in which the DNA binding domain of multiple FLI monomers function as independent binding units to facilitate transcriptional activity in a length-dependent manner. The EWS portion appears to be critical for in vivo binding of genomic DNA globally. We propose that GGAA microsatellites are necessary and sufficient for EWS/FLI-mediated oncogenic transformation and that repeat length affects optimal DNA binding stoichiometry and transcriptional activity.
Citation Format: Kirsten M. Johnson, Cenny Taslim, Stephen L. Lessnick. EWS/FLI regulates transcriptional activation via length-dependent GGAA microsatellites [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 3509. doi:10.1158/1538-7445.AM2017-3509</jats:p
Life at war and the heroic illusions created to cope with war: a study of Stephen Crane and Tim O'Brien
No full textThis thesis will examine the fictional war novels, The Red Badge of Courage by Stephen Crane and Going after Cacciato by Tim O‘Brien. It will examine the heroic illusions created by soldiers on the frontline as psychological coping mechanisms as a means to escape the realities of war. It will also examine how Stephen Crane and Tim O‘Brien create protagonists and characters that struggle to understand the conflicts within themselves as consequences of their developing point of view toward themselves, their war comrades, and their society‘s values and how each of these writers through observing battlefield experience comes to question the meaning of war and its effects. Stephen Crane and Tim O‘Brien investigate the moral and cultural values of their respective societies. Crane portrays the Victorian era O‘Brien examines1960‘s America. Each novel asks us to view their war with both irony and sympathy.M.A.L.S.Includes bibliographical referencesby Gaye L. Alle
Stephen King Day, Public Talk
No full textStephen King, the author of this year\u27s University of Maine cl-ass book, Hearts in Atlantis, has agreed. to come to campus on October l. To honor Stephen King and to provide a context for this year\u27s class book (part of which is set on our campus when Stephen was a student here in the 1960\u27s), Wednesday October 3 is being designated Stephen King Day. A number of activities are being planned., including a public talk
Stephen Leacock: A Reappraisal
No full textThis collection of essays explores the many dimensions of the writings of Stephen Leacock, the well-loved Canadian author of Sunshine Sketches of a Little Town.1. Riding Off in All Directions: A Few Wild Words in Search of Stephen Leacock (TIMOTHY FINDLEY)
2. Leacock and Leahen: The Feminine Influence on Stephen Leacock (ERIKA RITTER)
3. Leacock and Understanding Canada (GUY VANDERHAEGHE)
4. Leacock and the Media (RALPH L. CURRY)
5. The Historical Leacock (IAN ROSS ROBERTSON)
6. Stephen Leacock, Economist: An Owl Among the Parrots (MYRON J. FRANKMAN)
7. Imperial Cosmopolitanism, or the Partly Solved Riddle of Leacock’s Multi-National Persona (JAMES STEELE)
8. Stephen Leacock, Humorist: American by Association (BEVERLY RASPORICH)
9. Religion and Romance in Mariposa (GERALD LYNCH)
10. The Roads Back: Sunshine Sketches of a Little Town and George Elliott’s The Kissing Man (CLARA THOMAS)
11. Untestable Inferences: Post-Structuralism and Leacock’s Achievement in Sunshine Sketches of a Little Town (ED JEWINSKI)
12. The Achievement of Stephen Leacock (ALEC LUCAS, MALCOLM ROSS, GLENN CLEVER, R. L. MCDOUGALL)
13. Stephen Leacock: The Writer and His Writings (Compiled by RALPH L. CURRY
Stephen Leacock: A Reappraisal
No full textThis collection of essays explores the many dimensions of the writings of Stephen Leacock, the well-loved Canadian author of Sunshine Sketches of a Little Town.1. Riding Off in All Directions: A Few Wild Words in Search of Stephen Leacock (TIMOTHY FINDLEY)
2. Leacock and Leahen: The Feminine Influence on Stephen Leacock (ERIKA RITTER)
3. Leacock and Understanding Canada (GUY VANDERHAEGHE)
4. Leacock and the Media (RALPH L. CURRY)
5. The Historical Leacock (IAN ROSS ROBERTSON)
6. Stephen Leacock, Economist: An Owl Among the Parrots (MYRON J. FRANKMAN)
7. Imperial Cosmopolitanism, or the Partly Solved Riddle of Leacock’s Multi-National Persona (JAMES STEELE)
8. Stephen Leacock, Humorist: American by Association (BEVERLY RASPORICH)
9. Religion and Romance in Mariposa (GERALD LYNCH)
10. The Roads Back: Sunshine Sketches of a Little Town and George Elliott’s The Kissing Man (CLARA THOMAS)
11. Untestable Inferences: Post-Structuralism and Leacock’s Achievement in Sunshine Sketches of a Little Town (ED JEWINSKI)
12. The Achievement of Stephen Leacock (ALEC LUCAS, MALCOLM ROSS, GLENN CLEVER, R. L. MCDOUGALL)
13. Stephen Leacock: The Writer and His Writings (Compiled by RALPH L. CURRY
Self-consciousness and the image of self in the poetry of Stephen Spender, 1928 to 1934
No full textThe purpose of this thesis is twofold. First, to demonstrate the value and significance of Spender's early poetry in terms of its vision and technique. Through a series of close readings the thesis traces the ways in which Spender's early poetry not only shows itself to be self-conscious but also manipulates images of self. Presenting images of self, Spender achieves a balance between engagement with and distance from the self, and the reader shares in the process of poetic self-awareness. Secondly, to demonstrate the broader value of the poetry. Spender's poetry presents a distinctive exploration of the possibilities of self in relation to the external world. The resolution of Spender’s questioning and selection of both personal and public values, rooted in his contemporary situation and private circumstances, in his poetry takes the form less of historical document than of human record. The period on which I focus, 1928 to 1934, represents Spender’s first, and arguably most significant, poetic phase. The thesis is specifically concerned with four texts: Nine Experiments. Spender's contributions to Oxford Poetry (1929 and 1930), Twenty Poems and Poems (1933 and 1934). Nine Experiments marks the beginning of a particular approach and lyric style which finds its culmination in Poems (1933 and 1934). The earliest poetry is interesting largely insofar as it looks forward to later themes and techniques. In Nine Experiments and Oxford Poetry (1929 and 1930) we see Spender's often successful struggle to achieve effective forms in which to explore issues of self and value. Twenty Poems and Poems (1933 and 1934) concentrate on themes of love and friendship and the pressure on the poet of the contemporary political scene. The poetry does not reconcile the demands of the external, public world with his inner desires and aspirations, but presents a series of fascinatingly unresolved tensions. The thesis explores the way these poems strive for certainty. This striving stems from the tension between Spender's desire to politicize poetry and his tendency to the lyrical, personal statement
The Democratic State
No full textRoger Benjamin was president of the Council for Aid to Education (CAE) from 2005 to 2019 and was formerly provost of the University of Minnesota and the University of Pittsburgh. He has authored, coauthored, or co-edited nine books, including The Democratic Purposes of Education and The New Limits of Education Policy: Avoiding a Tragedy of the Commons.
Stephen L. Elkin is professor emeritus of government and politics at the University of Maryland and founding editor of the journal The Good Society. He is the author or editor of seven books, including Reconstructing the Commercial Republic: Constitutional Design after Madison.This Kansas Open Books title is funded by a grant from the National Endowment for the Humanities and the Andrew W. Mellon Foundation Humanities Open Book Program.Edited by Roger Benjamin and Stephen L. Elkin. Contributors include Peter H. Aranson, Roger Benjamin, David Braybrooke, Stephen L. Elkin, Norman Furniss, and Peter C. Ordeshook.One outcome of the declining economic growth and rising political conflict of the 1980s has been a renewed interest in political theory and increased questioning about the durability of the capitalist state. More and more political scientists are critically assessing the prevailing pluralist vision of the relationships between the state and the economy. Is the capitalist state able to adjust to crises and contradictions? What is the role of the state in changing—deteriorating—economic circumstances? How should we understand competing interpretations on the relative autonomy of the state, the nature of property rights, the legitimation crisis?
This collection of five original essays by seven of the best-known political-economy theorists addresses the interconnections between the economy and the polity and embodies the leading theoretical approaches to the political economy of the state
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