1,517 research outputs found
Association between HLA and islet cell antibodies in diabetic patients with a mitochondrial DNA mutation at base pair 3243
No full textIslet cell antibodies (ICA), autoantibodies to glutamic acid decarboxylase (GAD) and HLA genotypes were examined in 31 patients with diabetes and a mitochondria! gene mutation located at base pair 3243 (mtDNA 3243 mutation). ICA was detected in 42 % (13/31) of these patients compared to 0 of 90 among healthy control subjects. The ICA showed a "non-restricted" pattern of staining in all 13 ICA-positive patients. In a sensitive radioligand assay only 2 of 31 (6 %) diabetic patients with the mutation were positive for both GAD65 autoantibodies and ICA, while the remaining 29 patients were GAD65 antibody negative. The ICA-positive patients had an increased frequency of the HLADQA1*0301 allele compared to control subjects (p < 0.05). Of the diabetic patients with the mutation 45% (14/31) had progressive clinical course of betacell failure. These results indicate thai patients with an mtDNA 3243 mutation may develop islet autoimmunity associated with ICA and GAD autoantibodies. We hypothesize that the presence of HLADQA1*0301 in individuals with the mtDNA 3243 mutation increases the risk for diabetes and associated autoantibodies against islet cell antieens
A POSSIBLE ROLE OF THE PANCREATIC α1- AND α2-CELLS AS LOCAL REGULATORS OF INSULIN SECRETION
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Room temperature structures beyond 1.5 Å by serial femtosecond crystallography
No full textabstract: About 2.5 × 10[superscript 6] snapshots on microcrystals of photoactive yellow protein (PYP) from a recent serial femtosecond crystallographic (SFX) experiment were reanalyzed to maximum resolution. The resolution is pushed to 1.46 Å, and a PYP structural model is refined at that resolution. The result is compared to other PYP models determined at atomic resolution around 1 Å and better at the synchrotron. By comparing subtleties such as individual isotropic temperature factors and hydrogen bond lengths, we were able to assess the quality of the SFX data at that resolution. We also show that the determination of anisotropic temperature factor ellipsoids starts to become feasible with the SFX data at resolutions better than 1.5 Å.The final version of this article, as published in Structural Dynamics, can be viewed online at: http://aca.scitation.org/doi/10.1063/1.491990
Progression from islet autoimmunity to clinical type 1 diabetes is influenced by genetic factors
No full textR code for the statistical analysis of the following publication:
Beyerlein A, Bonifacio E, Vehik K, Hippich M, Winkler C, Frohnert BI, Steck AK, Hagopian WA, Krischer JP, Lernmark Å, Rewers MJ, She JX, Toppari J, Akolkar B, Rich SS, Ziegler AG; TEDDY Study Group: Progression from islet autoimmunity to clinical type 1 diabetes is influenced by genetic factors: results from the prospective TEDDY study. J Med Genet. 2019; 56(9):602-60
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