1,720,970 research outputs found
Mechanism of De-energized Mitochondria Membrane Potential Formation in Permeabilized Cardiac Myocytes
No full textThe biophysical characteristics in de-energized mitochondria are not known very well. In this study, we carried out the investigation into the membrane potential formation at de-energized states of mitochondria. NADH, FAD and ΔΨm were monitored simultaneously using permeabilized cardiac myocytes of the rat. We showed de-energized mitochondria in absence of mitochondrial substrates still have a membrane potential and it was about 56 ± 5 mV. To pursue the mechanism of that, firstly we tried the possibility of the involvement of mito-KATP channel. We tested effects of Pi /ATP, KATP channel blockers/opener, and K+ replacement with meglumine on the de-energized mitochondria. The addition of Pi and ATP could dramatically hyperpolarized about 62 ± 7.7 mV. However, all agents blocking KATP channel did not show any significant effects. Interestingly, Diazoxide (DZX), KATP channel opener, and Oligomycin A (OligoA), F1-F0-ATPase inhibitor, itself could depolarize the Ψm in basal condition about 11 ± 1 mV and 46.5 ± 6 mV respectively, and the addition of Pi could reverse the effect. The ATP induced the hyperpolarization was occurred with DZX but was fully inhibited by OligoA. K+ replacement with meglumine slowed down the speed of ATP induced hyperpolarization and made it transient. However, the change NMDG to K+ could reverse and showed the full effect of ATP on Ψm. From these results, KATP channel and reverse mode of F1-F0-ATPase by the residual ATP hydrolysis might contribute the formation of the resting Ψm in de-energized mitochondria even though the mechanism of formation of the resting Ψm is still not clear. The K+ environment seemed to be important for the activity of F1-F0-ATPase.
Key Words: Ψm ,mito-KATP channel, ATP, Diazoxide, Oligomycin AMaste
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Perspectives of physiome research
No full textAbstractPhysiome is an area of physiology to generate a whole system by analyzing and integrating scattered and discrete information. The term “physiome” was first introduced by James B. Bassingthwaight in 1993, and officially announced by the International Union of Physiological Societies as the new field to be accomplished in the 21st century. In this review, I introduce the concepts of physiome, why physiome should be pursued, what kind of strategy is necessary to form physiome, and how physiome can be used
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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