5,602 research outputs found
CHO microRNA engineering is growing up : recent successes and future challenges
No full textmicroRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines
Erratum: 3D bioprinted in vitro secondary hyperoxaluria model by mimicking intestinal-oxalatemalabsorption-related kidney stone disease (Applied Physics Reviews (2022) 9 (041408) DOI: 10.1063/5.0087345)
No full text© 2023 Author(s).This article was originally published online on 21 November 2022 with an incorrect affiliation identifier for author Dong-Woo Cho. It is correct as it appears above. All online versions of this article were corrected on 23 November 2022. AIP Publishing apologizes for this error.11Nsciescopu
Unimodality of Betti numbers for Hamiltonian circle actions with index-increasing moment Maps
No full textThe unimodality conjecture posed by Tolman in [L. Jeffrey, T. Holm, Y. Karshon, E. Lerman and E. Meinrenken, Moment maps in various geometries, http://www.birs.ca/workshops/2005/05w5072/report05w5072.pdf] states that if (M,ω) is a 2n-dimensional smooth compact symplectic manifold equipped with a Hamiltonian circle action with only isolated fixed points, then the sequence of Betti numbers {b0(M),b2(M),...,b2n(M)} is unimodal, i.e. bi(M) ≤ bi+2(M) for every i < n. Recently, the author and Kim [Y. Cho and M. Kim, Unimodality of the Betti numbers for Hamiltonian circle action with isolated fixed points, Math. Res. Lett. 21(4) (2014) 691-696] proved that the unimodality holds in eight-dimensional case by using equivariant cohomology theory. In this paper, we generalize the idea in [Y. Cho and M. Kim, Unimodality of the Betti numbers for Hamiltonian circle action with isolated fixed points, Math. Res. Lett. 21(4) (2014) 691-696] to an arbitrary dimensional case. We prove the conjecture in arbitrary dimension under the assumption that the moment map H : M → R is index-increasing, which means that ind(p) < ind(q) implies H(p) < H(q) for every pair of critical points p and q of H, where ind(p) is the Morse index of p with respect to H. © World Scientific Publishing Company1111sciescopu
Systems biology for reverse aging
No full textCellular senescence is an irreversible and permanent cell cycle arrest in response to internal and external stresses. Its unresponsiveness to growth factor signals distinguishes it from a potentially reversible state, quiescence. Cellular senescence can inhibit tumor development by blocking proliferation of damaged cells, but as senescent cells become accumulated in a tissue, they can contribute to the promotion of agerelated diseases such as cancer by secreting inflammatory cytokines [1]. © 2021 Cho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
The antihypertensive effects of the Jamaican Cho-Cho (Sechium edule)
No full textThe experiments reported in this study constitute a preliminary investigation into the possible hypotensive effect of the Jamaican Cho-Cho (Sechium edule). Experiments were conducted in a random and blind fashion on two sub species of Sechium edule. Both the pulp and the peel were examined for hypotensive activity. Water-soluble extracts were prepared from these components of the fruit and injected into anaesthetised rats. Various cardiovascular parameters were measured including heart rate, mean arterial pressure (MAP) and several ECG intervals. We report that all extracts tested produced a fall in blood pressure with little change in ECG intervals. Extract B produced the least change in heart rate with a fall in MAP of approximately 23 mmHg. Changes in heart rate with all extracts appeared to be minimal as an ED25 value could only be determined for extract A, and ED10 values could not be evaluated for extracts C and D. The mechanism(s) by which these extracts produce their hypotensive effects could not be determined in these preliminary experiments. However, it appears not to involve direct effects on cardiac tissue. This conclusion is based on the finding that it took a minimum of 10 to 15 seconds for the hypotensive action to manifest post bolus. Future experiments will be aimed at delineating the mechanism(s) involved in decreasing MAP.Peer reviewedfinal article publishe
Nota su Eschilo, Cho. 65
No full textThe author defends the reading ἄκραντος in the sense of “unfinished” in Aesch. Cho. 65
High spatial resolution microwave detection system for long range Brillouin-based distributed sensors
No full textWe present a microwave detection system for long range Brillouin-based sensing with a potential spatial resolution of 60cm. It was demonstrated over 30km, with temperature accuracy of 1.6C, and spatial resolution of 2m
Surface Selective Oxidation, Hot Dip Galvanizing and Coating-related Properties of Advanced High Strength and Ultra-high Strength Automotive Steel Grades
No full textHigh spatial resolution microwave detection system for Brillouin-based distributed temperature and strain sensors
No full textWe present a microwave detection system for coherent detection of spontaneous Brillouin-based distributed temperature and strain measurements. The system was designed to overcome the existing bandwidth limitations of a previously used commercial spectrum analyser and provide a commercially practical solution to the detection of the Brillouin frequency shift and intensity for long range high spatial resolution measurements. The detection system bandwidth corresponds to a potential spatial resolution of ~60 cm. The system was demonstrated as a temperature sensor over a range of 30 km, with a temperature resolution of 1.6 °C and spatial resolution of ~2 m. It was also demonstrated as a combined temperature and strain sensor over a range of 6.3 km with ~1.3 m spatial resolution; temperature and strain resolutions of 3 °C and 80 µε respectively were achieved
Surface Selective Oxidation, Hot-Dip Galvanizing and Coating-Related Properties of Advanced High-Strength and Ultrahigh-Strength Automotive Steel Grades
No full text[No abstract available]11scopu
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