95 research outputs found
Development of an analytical protocol for a fast, sensitive and specific protein recognition in paintings by enzyme-linked immunosorbent assay (ELISA)
No full textAnal Bioanal Chem. 2011 Mar;399(9):3011-23. Epub 2010 Dec 19.
Development of an analytical protocol for a fast, sensitive and specific protein recognition in paintings by enzyme-linked immunosorbent assay (ELISA).
Palmieri M, Vagnini M, Pitzurra L, Rocchi P, Brunetti BG, Sgamellotti A, Cartechini L.
SourceCentro SMAArt, Dipartimento di Chimica, Università di Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy.
Abstract
Enzyme-linked immunosorbent assay (ELISA) analysis of proteins offers a particularly promising approach for investigations in cultural heritage on account of its appreciated properties of being highly specific, sensitive, relatively fast, and cost-affordable with respect to other conventional techniques. In spite of that, it has never been fully exploited for routine analyses of painting materials in consideration of several analytical issues that inhibited its diffusion in conservation science: limited sample dimensions, decrease of binder solubility and reduced availability of antibody bonding sites occurring with protein degradation. In this study, an ELISA analytical protocol suited for the identification of aged denatured proteins in ancient painting micro-samples has been developed. We focused on the detection of bovine β-casein and chicken ovalbumin as markers of bovine milk (or casein) and chicken albumen, respectively. A systematic experimentation of the ELISA protocol has been carried out on mock-ups of mural and easel painting prepared with 13 different pigments to assess limits and strengths of the method when applied for the identification of proteins in presence of a predominant inorganic matrix. The analytical procedure has been optimized with respect to protein extraction, antibodies' concentrations, incubation time and temperature; it allows the detection of the investigated proteins with sensitivity down to nanograms. The optimized protocol was then tested on artificially aged painting models. Analytical results were very encouraging and demonstrated that ELISA allows for protein analysis also in degraded painting samples. To address the feasibility of the developed ELISA methodology, we positively investigated real painting samples and results have been cross-validated by gas chromatography-mass spectrometry.
PMID: 2117052
Immunochemical Methods Applied to Art-Historical Materials: Identification and Localization of Proteins by ELISA and IFM
No full textDespite the large diffusion of natural organic substances in art-historical materials, their characterization presents many challenges due to the chemical complexity and instability with respect to degradation processes. Among natural products, proteins have been largely used in the past as binders but also as adhesives or additives in coating layers. Nevertheless, biological identification of proteins in art-historical objects is one of the most recent achievements obtained in heritage science thanks to the development of specifically tailored bio-analytical strategies. In the context of this active emerging discipline, immunological methods stand out for sensitivity, specificity and versatility for both protein recognition and localization in micro-samples. Furthermore, the growing use of immunological techniques for advanced diagnostics and clinical applications ensures continuous improvement in their analytical performance. Considering such, this review provides an overview of the most recent applications of enzyme linked immunosorbent assay and immunofluorescence microscopy techniques in the field of heritage materials. Specifically, the main strengths and potentials of the two techniques as well as their limits and drawbacks are presented and discussed herein
O reaction
No full textFollowing a recent investigation on the N(2D) + H2O reaction [Homayoon et al., J. Phys. Chem. Lett. 5, 3508 (2014)], we
report on an experimental and theoretical study of the isotopologue N(2D) + D2O reaction. Crossed molecular beam (CMB)
experiments were conducted at a collision energy of 10.3 kcal mol–1. Quasiclassical trajectory calculations were performed
on a recent potential energy surface to derive the centre-of-mass functions necessary to simulate the CMB laboratory
distributions. Excellent agreement was found. The importance of the channel leading to HON/DON was confirmed. The
inclusion of this channel, in addition to that leading to the isomer HNO/DNO, can affect the models considering the coupling
between nitrogen and oxygen chemistry in the upper atmosphere of Titan
On the Formation of Nitrogen-Bearing Molecules in Extraplanetary Atmospheres via Neutral Reactions : A Crossed Beam Study of N(2D)+C2H2
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Glancing-incidence X-ray diffraction of Ag nanoparticles in gold lustre decoration of Italian Renaissance pottery
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Crossed Beam Reactive Scattering of Nitrogen Atoms: the Reaction Dynamics of N( 2 D) + CH 4 and N( 2 D) + H 2 O
No full textExperimental studies on elementary reactions of C atoms and CN and OH radicals with molecules of importance in the interstellat medium
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