341 research outputs found
Induction of heme oxygenase-1 by traditional Chinese medicine formulation ISF-1 and its ingredients as a cytoprotective mechanism against oxidative stress
No full textTraditional medicines are clinically used to treat post-stroke disorders in China. In search of alternative medicines for post-stroke rehabilitation, we recently identified the heme oxygenase-1 (HO-1) pathway as a key mechanism underlying the biological activities of the ischemic stroke formulation ISF-1. This study was designed to further investigate ISF-1 for HO-1 induction in cultured human cells and corresponding cytoprotective effects against oxidative injury. A rat stroke model induced by middle cerebral artery occlusion was employed to verify the activity of ISF-1 in vivo. It was found that HO-1 expression was induced by ISF-1 in a dose- and time-dependent manner. Four ingredients from ISF-1 were identified to be responsible for HO-1 induction. The appropriate combinations of these active ingredients or purified compounds resulted in synergistic induction of HO-1 expression. A minimal HO-1-inducing formulation was prepared and showed significant cytoprotection against H 2O 2-induced oxidative stress. Collectively, the herbal formulation ISF-1 was capable of inducing HO-1 expression, in vitro and in vivo, offering a potential mechanism for post-stroke rehabilitation. This study may shed light on the development of mechanism-defined therapies based on traditional herbal remedies.link_to_subscribed_fulltex
Author correction: Microneedle-based biosensing
No full textCorrection to: Nature Reviews Bioengineering https://doi.org/10.1038/s44222-023-00108-7, published online 26 September 2023.In the version of the article initially published, in Fig. 4bv, the left y axis read “ISF alcohol (mM)” and has now been corrected to “ISF lactate (mM)” in the HTML and PDF versions of the article
Genome-wide biological response fingerprinting (BioReF) of the Chinese botanical formulation ISF-1 enables the selection of multiple marker genes as a potential metric for quality control
No full textQuality control plays a critical role in the process of translating the traditional/alternative medicines into modern evidence-based therapies. High performance liquid chromatography (HPLC) is widely applied to assess the chemical composition of botanical drug products. The chromatographic fingerprints or chemical profiles are currently used as the de facto quality control metric. As a complement to chemical profiles, a biological quality control assessment offers distinct advantages. This study describes a genome-wide biological response fingerprinting (BioReF) approach to define a set of marker genes that define a signature pattern for a specific botanical formulation. These marker genes are chosen on the basis of the levels of the regulated expression and the involvement in the cellular signaling pathways. Subsequently, qRT-PCR technique is used to simultaneously monitor the gene expression of multiple marker genes in an efficient and quantitative manner. This set of marker genes represents the biological responses of human cells to the chemical composition of the botanical drug that could serve as potential quality control of botanical drugs in terms of the consistency of biological activities. We demonstrate the BioReF approach with a well-documented Chinese Medicine formula, designated as ISF-1, traditionally used for the management of post-stroke disorders. A set of nine marker genes were selected to assess the batch-to-batch consistency of the biological effects of ISF-1. This approach provides a potential comprehensive and cost-effective quality control metric of the biological activities of botanical drugs
Architectural setup for online monitoring and control of process parameters in robot-based ISF
No full textMethodology for Process Improvement Through Basic Components and Focusing on the Resistance to Change.
No full textThis paper describes a multi-model methodology that implements a smooth and continuous process improvement, depending on the organization's business goals and allowing users to establish their improvement implementation pace. The methodology focuses on basic process components known as ‘best practices’. Besides, it covers following the topics: knowledge management and change management. The methodology description and the results of a case study on project management process are included
Springback Reduction by Annealing for Incremental Sheet Forming
No full textAbstractIncremental sheet forming (ISF) has gained much attention in the low volume production and rapid prototyping fields due to its inherent flexibility, low-overhead cost, and die-less nature. However, the geometric inaccuracy of the final achievable parts hinders this process from becoming commercially viable. One of the major sources of geometric inaccuracy is springback, which predominately occurs when the formed sheet metal is unloaded by the forming tools and released from the clamps. This paper focuses on the latter case. A practical annealing method that does not rely on complicated setups and geometry-specific compensation algorithms is proposed to reduce the springback that occurs after the unclamping process. Two clamping devices were developed, which have been demonstrated to significantly reduce the amount of springback. The capability of reducing springback by the proposed method and its potential of integrating it with other geometric accuracy improvement methods will enhance the ISF process towards future industrial applications
A novel hybrid coagulation-intermittent sand filter for the treatment of dairy wastewater
No full textAn intermittent sand filter (ISF) is a simple and cost-effective treatment method that may be adopted on farms to treat dairy wastewater (DWW). However, the use of ISFs has been limited due to the large area required for treatment, and the risk of filter clogging and phosphorus (P) breakthrough, which decrease the operational lifetime. To overcome these limitations, this study uses a novel, pilot-scale coagulation-sedimentation process prior to loading ISFs with DWW. The performance and operational lifetime of this new hybrid coagulation-ISF system was compared to a conventional ISF system in a replicated outdoor pilot-scale experiment over a 43-wk study duration (covering an entire milking season on a farm in Ireland). The hybrid system was able to operate effectively at a higher hydraulic loading rate than a conventional ISF system. The effluent quality from the conventional ISF deteriorated over the timeframe of the study until clogging occurred, while the hybrid system continued to perform effectively without any evidence of clogging or P breakthrough. The hybrid system obtained removal efficiencies ≥99% for all measured water quality parameters (chemical oxygen demand, total suspended solids, total P, ammonium and turbidity), and complied with EU directives concerning urban wastewater treatment. Overall, the hybrid coagulation-ISF is a promising technology that requires a small area (75% reduction in footprint in comparison to a conventional ISF) and minimal operator input, and produces high effluent quality.The authors are grateful to Teagasc for the award of a Walsh Fellowship to the first author [grant number: RMIS-0386]. The authors appreciate the help of technical staff: Adrian Hawe; Seamus McShane, JohnPaul Murphy & Tomas Condon (Teagasc Moorepark), and Denis Brennan (Teagasc Johnstown Castle).peer-reviewe
Transportation in the Interstitial Space of the Brain Can Be Regulated by Neuronal Excitation
No full textThe transportation of substances in the interstitial space (ISS) is crucial for the maintenance of brain homeostasis, however its link to neuronal activity remains unclear. Here, we report a marked reduction in substance transportation in the ISS after neuronal excitation. Using a tracer-based method, water molecules in the interstitial fluid (ISF) could be specifically visualized in magnetic resonance (MR) imaging. We first observed the flow of ISF in the thalamus and caudate nucleus of a rat. The ISF flow was then modulated using a painful stimulation model. We demonstrated that the flow of ISF slowed significantly following neuronal activity in the thalamus. This reduction in ISF flow continued for hours and was not accompanied by slow diffusion into the ISS. This observation suggests that the transportation of substances into the ISS can be regulated with a selective external stimulation.National Natural Science Foundation of China [81171080, 81471633, 91330103, 61450004, 61471005]SCI(E)[email protected]
Biomechanics of L5/S1 in Long Thoracolumbosacral Constructs: A Cadaveric Study
No full text© The Author(s) 2018. Study Design: In vitro cadaveric biomechanical study. Objectives: Despite numerous techniques employed to establish solid lumbosacral fixation, there are little biomechanical data correlating fixation methods at L5/S1 to thoracolumbosacral (TLS) construct length. We aimed to determine the optimal construct with the hypothesis that under physiological loads, lumbosacral constructs can be stabilized by L5/S1 anterior lumbar interbody fusion (ALIF) alone, without iliac screw fixation (ISF), and that TLS constructs would require ISF, with or without ALIF. Methods: By using a robot capable of motion in 6 axes, force-moment sensor, motion-tracking camera system and software, we simulated the spinal loading effects in flexion-extension, axial rotation, and lateral bending, and compared torques in different construct groups of T4-S1, T10-S1, and L2-S1. By conducting multidirectional flexibility testing we assessed the effects of constructs of various lengths on the L5/S1 segment. Results: L2-S1 constructs may be equivalently stabilized by L5/S1 ALIF alone without ISF. Longer TLS constructs exerted increasing motion at L5/S1, exhibiting trends in favor of ISF when extending to T10 and statistically improved fixation when extending to T4. Lastly, TLS constructs with ISF exhibited a statistically significant reduction in L5-S1 range of motion from the addition of ALIF when extending to T4-pelvis but not T10-pelvis. Conclusions: We found that ALIF alone may sufficiently support the L2-S1 construct, reducing L5/S1 range of motion and transmitting loads instead to the sacropelvis. Furthermore, ALIF was found to add significant stability to the T4-pelvis construct when added to ISF. This difference was not significant for the T10-pelvis construct
A modular tooling set-up for incremental sheet forming (ISF) with subsequent stress-relief annealing under partial constraints
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