2,981 research outputs found
NMR analysis reveals extensive binding interactions of complex xyloglucan oligosaccharides with the Cellvibrio japonicus Glycoside Hydrolase Family 31 -xylosidase
No full textThe study of the interaction of glycoside hydrolases with their substrates is fundamental to diverse applications in medicine, food and feed production, and biomass-resource utilization. Recent molecular modeling of the a-xylosidase CjXyl31A from the soil saprophyte Cellvibrio japonicus, together with protein crystallography and enzyme-kinetic analysis, has suggested that an appended PA14 protein domain, unique among glycoside hydrolase family 31 members, may confer specificity for large oligosaccharide fragments of the ubiquitous plant polysaccharide xyloglucan (J. Larsbrink, A. Izumi, F. M. Ibatullin, A. Nakhai, H. J. Gilbert, G. J. Davies, H. Brumer, Biochem. J. 2011, 436, 567580). In the present study, a combination of NMR spectroscopic techniques, including saturation transfer difference (STD) and transfer NOE (TR-NOE) spectroscopy, was used to reveal extensive interactions between CjXyl31A active-site variants and xyloglucan hexa- and heptasaccharides. The data specifically indicate that the enzyme recognizes the entire cello-tetraosyl backbone of the substrate and product in positive enzyme subsites and makes further significant interactions with internal pendant a-(1?6)-linked xylosyl units. As such, the present analysis provides an important rationalization of previous kinetic data on CjXyl31A and unique insight into the role of the PA14 domain, which was not otherwise obtainable by protein crystallography.</p
Report of Governor Johan Rising, 1655, on New Sweden
No full textGovernor Johan Rising reports to the Swedish government and royalty on the status of New Sweden (present-day New Jersey). He also reports on other Swedish colonies in the area. He asks that single women and skilled tradesmen be sent to the colonies. Rising also reports that attacks from neighboring Indian tribes are increasing. He has found some protection by forming an alliance with English settlers, but the cost is high, and his colony owes the English money and supplies. Rising asks that Sweden send them money so that they can pay off their debts, build ships that would establish a trading dominance with the West Indies, and cultivate land and crops to gain more profit. Reports from New World governors were sent back to their native countries via ships. Rising sent this report in June of 1655, but Sweden did not recieve the report until November of 1655. This article is part of a Primary Source Material collection compiled by the New Sweden Commemorative Commission in 1988
Report of Governor Johan Printz, 1647, on New Sweden
No full textGovern Johan Printz, the governor of New Sweden (later to become New Jersey), reports on the status of the colony and the settlers. Many freemen have arrived to settle in New Sweden, but the criminals and military men who were conscripted to the colony want to return to Sweden. Of the freemen, very few are skilled, so Governor Printz asks that blacksmiths, tanners, tailors, carpenters, and butchers be sent to the colony. Additionally, he asks for single women. Printz also reports on two new Swedish colonies that have been established along the Delaware River. However, Dutch settlers have become very aggressive by re-purchasing land from the Indians that the Swedish had already bought. They are also interrupting trade between the Swedes and the Indians, as well as instigating the Indians to attack the Swedes. Printz directed the construction of some storage houses along common trade routes to win back trade from the Indians. However, fighting has erupted between different Native tribes as each tries to establish dominance in trading with the colonies. This article is part of a Primary Source Material collection compiled by the New Sweden Commemorative Commission in 1988
Relation of the surrender of New Sweden, by Governor Johan Clason Rising, 1655
No full textGovernor Johan Rising of New Sweden reports to Sweden on the August 1655 Dutch attack on New Sweden's Fort Christina. The Dutch traveled from New Amsterdam (present-day New York) and easily captured a New Sweden outpost. Rising sent men to the outpost to fend of the Dutch, but the Dutch defeated them and took them as prisoners, leaving Fort Christina without fighting men and supplies. The Dutch then put the Fort under seige. A few days later, Rising surrendered the Fort. He and the Swedish colonists were ordered to either return to Sweden, or to remain in the New World in service to the Dutch. This article is part of a Primary Source Material collection compiled by the New Sweden Commemorative Commission in 1988
The past, present and future of social media in project management
No full textGreen Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Integral Design & Managemen
Exploration of three <i>Dyadobacter fermentans </i>enzymes uncovers molecular activity determinants in CE15
No full textAbstract: Glucuronoyl esterases (GEs) are serine-type hydrolase enzymes belonging to carbohydrate esterase family 15 (CE15), and they play a central role in the reduction of recalcitrance in plant cell walls by cleaving ester linkages between glucuronoxylan and lignin in lignocellulose. Recent studies have suggested that bacterial CE15 enzymes are more heterogeneous in terms of sequence, structure, and substrate preferences than their fungal counterparts. However, the sequence space of bacterial GEs has still not been fully explored, and further studies on diverse enzymes could provide novel insights into new catalysts of biotechnological interest. To expand our knowledge on this family of enzymes, we investigated three unique CE15 members encoded by Dyadobacter fermentans NS114T, a Gram-negative bacterium found endophytically in maize/corn (Zea mays). The enzymes are dissimilar, sharing ≤ 39% sequence identity to each other‚ and were considerably different in their activities towards synthetic substrates. Combined analysis of their primary sequences and structural predictions aided in establishing hypotheses regarding specificity determinants within CE15, and these were tested using enzyme variants attempting to shift the activity profiles. Together, the results expand our existing knowledge of CE15, shed light into the molecular determinants defining specificity, and support the recent thesis that diverse GEs encoded by a single microorganism may have evolved to fulfil different physiological functions. Key points: • D. fermentans encodes three CE15 enzymes with diverse sequences and specificities • The Region 2 inserts in bacterial GEs may directly influence enzyme activity • Rational amino acid substitutions improved the poor activity of the DfCE15A enzyme.</p
An operationalization of Stevenson’s conceptualization of entrepreneurship as opportunity-based firm behavior
No full textThis is the author-version of article published as: Brown, Terrence and Davidsson, Per and Wiklund, Johan (2001) An operationalization of Stevenson’s conceptualization of entrepreneurship as opportunity-based firm behavior. Strategi
Social media for improving metro rail project operations
No full textGreen Open Access added to TU Delft Institutional Repository 'You share, we take care!' - Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Integral Design & Managemen
The social challenges of low carbon development
No full textThis book explores the social implications and challenges of low-carbon development. The argument of the book is that a broad understanding of low-carbon development is essential for mitigating climate change and enabling development in a carbon-constrained world, but there are risks that low-carbon development might come at a price that is both social and economic. These risks need to be carefully assessed and reduced. The main aim of the book is to explore, critically analyse and propose different ways of understanding low-carbon development from a social perspective in both developed and developing countries. The author uses concepts such as low-carbon development, social policy, sustainable development and environmental justice to understand the social implications of low-carbon development projects. The book first elaborates the need to understand the social issues and challenges of low-carbon development in both developed and developing countries. It then discusses five contemporary challenges of low-carbon development: * the social consequences of Chinese hydropower dams in the Mekong region; * the cost of the transition to renewable energies such as wind energy in Germany; * the challenges of carbon offsetting in Brazil; * the nexus of fuel-inefficient housing and fuel poverty in England; * solar power for refugees in Africa. The book fills a crucial gap for researchers, postgraduates, practitioners and policy-makers in the fields of climate change, development and social policy. Johan Nordensvard is a Lecturer in Social Policy at the University of Southampton, UK
Johan Risell Samlade dikter : Utgivna med inledning och kommentarer av Lars Burman
No full textJohan Risell, Samlade dikter. Utgivna med inledning och kommentarer av Lars Burman. (Johan Risell, Collected Poems. Edited with introduction and commentary by Lars Burman.) Skrifter utgivna av Svenska Vitterhetssamfundet. Svenska författare. Ny serie, xxxii + 296 p. Stockholm. ISBN 978-91-7230-213-6 Johan Risell (1697–1724) was a highly acclaimed Swedish poet during the early 18th century. However, he has gradually been forgotten, possibly because of his early death and the changing times and literary ideals, but mainly because only seven poems have been known to his name. During the 20th century, he did not receive attention.Due to a recent manuscript find in Karlstad in Värmland, 61 poems by Risell are now known. This volume presents a critical edition of his poems. Notwithstanding his death at the age of only 27, Risell is shown to have been a diligent and varied author, writing orations and occasional verse to further his career, but also excelling in satires and songs to entertain his friends. It is clear that he performed his orations and songs, and he appears as a Swedish example of an early modern singer-songwriter. The student setting of Uppsala was important. In many of his poems and songs he celebrates a joyful bohemian way of life, in others, he appears as a melancholic lover. His vivid imagination, emotional appeal and oral qualities are to be noted. The themes of love and war are prominent. His more than two dozen song-texts are particularly notable, as it is shown that many of these were widely spread during the 18th century, though without attribution. They appear in handwritten songbooks and a few were printed in chapbooks. Johan Risell was a vicar’s son from Värmland and Dalsland, who matriculated at Uppsala University 1717, but never received a degree. After working as a tutor for richer families, he finally was appointed »stadssekreterare« (a local administrative position calling for some training in law) in Köping. He died in Uppsala before he could take up office.Vetenskaplig textkritisk utgåva. Critical edition.</p
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