676 research outputs found
The effects of Cu and Fe availability on the growth and Cu : C ratios of marine diatoms
No full textWe investigated the effects of copper (Cu) and iron (Fe) availability on the growth rates, cellular Cu content, and steady-state Cu uptake rates of eight species of centric diatoms (coastal and oceanic strains). Whereas Fe and Cu availability had a significant effect on the growth rates of both costal and oceanic diatoms, an interaction between Fe and Cu availability and growth rates was only observed for the oceanic diatoms. Determination of cellular Cu : carbon (C) quotas using the radiotracers (67)Cu and (14)C revealed that under Cu-sufficient conditions oceanic diatoms had elevated Cu : C ratios relative to coastal strains, regardless of Fe availability. Two species (one oceanic and one coastal) significantly increased their Cu demands in response to Fe limitation, indicating upregulation of the Cu-dependent high-affinity Fe uptake system in these organisms. The changes in cellular Cu : C ratios were accompanied by variations in steady-state Cu uptake rates. Thus, in some cases Cu uptake rates appear to be regulated by the cell in response to Fe availability. Rates of Cu acquisition also responded significantly to Cu variability. The variation in Cu uptake was more closely correlated with changes in total Cu concentration in the medium than in inorganic, free Cu concentrations, implying that organic Cu complexes may be bioavailable to diatoms. These findings indicate a greater biological role for Cu than was previously thought in open ocean regions
Copper-uptake kinetics of coastal and oceanic diatoms
No full textWe investigated copper (Cu) acquisition mechanisms and uptake kinetics of the marine diatoms Thalassiosira oceanica Hasle, an oceanic strain, and Thalassiosira pseudonana Hasle et Heimdal, a coastal strain, grown under replete and limiting iron (Fe) and Cu availabilities. The Cu-uptake kinetics of these two diatoms followed classical Michaelis-Menten kinetics. Biphasic uptake kinetics as a function of Cu concentration were observed, suggesting the presence of both high- and low-affinity Cu-transport systems. The half-saturation constants (K(m)) and the maximum Cu-uptake rates (V(max)) of the high-affinity Cu-transport systems (similar to 7-350 nM and 1.5-17 zmol center dot mu m-2 center dot h-1, respectively) were significantly lower than those of the low-affinity systems (> 800 nM and 30-250 zmol center dot mu m-2 center dot h-1, respectively). The two Cu-transport systems were controlled differently by low Fe and/or Cu. The high-affinity Cu-transport system of both diatoms was down-regulated under Fe limitation. Under optimal-Fe and low-Cu growth conditions, the K(m) of the high-affinity transport system of T. oceanica was lower (7.3 nM) than that of T. pseudonana (373 nM), indicating that T. oceanica had a better ability to acquire Cu at subsaturating concentrations. When Fe was sufficient, the low-affinity Cu-transport system of T. oceanica saturated at 2,000 nM Cu, while that of T. pseudonana did not saturate, indicating different Cu-transport regulation by these two diatoms. Using CuEDTA as a model organic complex, our results also suggest that diatoms might be able to access Cu bound within organic Cu complexes
O MITO CLÁSSICO COMO FONTE LITERÁRIA: O RETORNO DE PÍRAMO E TISBE EM JOGOS VORAZES DE SUZANNE COLLINS: THE CLASSICAL MYTH AS LITERARY SOURCE: THE RETURN OF PYRAMUS AND THISBE IN THE HUNGER GAMES BY SUZANNE COLLINS
No full textRESUMO: Este trabalho dedica-se a evidenciar como Suzanne Collins, autora do romance Jogos vorazes (2010), utilizou elementos da mitologia greco-romana para compor partes significativas de sua obra, formando, assim, uma cadeia intertextual entre a Literatura Clássica e a Contemporânea. Para além de todos os mitos resgatados, atentar-nos-emos, mais especificamente, ao mito de Píramo e Tisbe, registrado literariamente por Ovídio em suas Metamorfoses, um dos precursores da afamada peça de teatro Romeu e Julieta, de Shakespeare. Pelo viés estilístico da intertextualidade, Collins (2010) recupera um mito e o transforma em algo adaptado à nova situação em que é inserido, favorecendo a intersecção entre mito e literatura e presentificando a Antiguidade Clássica na Atualidade.
PALAVRAS-CHAVE: Jogos vorazes. Suzanne Collins. Píramo e Tisbe. Ovídio. Intertextualidade.
ABSTRACT: This work focuses on how Suzanne Collins, author of the novel The Hunger Games(2010), used elements of Greco-Roman mythology to compose significant parts of her work, thus forming an intertextual chain between Classical and Contemporary Literature. In addition to all the myths the author rescued, we will study more specifically the myth of Pyramus and Thisbe, recorded literarily by Ovid in his Metamorphoses, one of the precursors of Shakespeare’s famous play Romeo and Juliet. Through the intertextual approach, Collins (2010) recovers a myth and transforms it into something adapted to the new situation in which it is inserted, favoring the intersection between myth and literature and bringing back the Classical Antiquity to the Present.
KEYWORDS: The Hunger Games. Suzanne Collins. Pyramus and Thisbe. Ovid. Intertextuality
Modern Frontier: Aspects of the 1950s in Australia's Northern Territory
No full text'Modern Frontier' is a study of Australia's Northern Territory in the 1950s using an interdisciplinary approach that takes in environmental, historical and cultural history. Through a series of chapters from a number of contributors, a decade in Australian history is revealed from a Territory perspective. The editors have brought together a diverse range of authors, experts in their fields, who provide a fascinating insight into aspects of Australian history and policy in the north. The decade that brought issues of assimilation and Aboriginal culture to the national stage, against a backdrop of the Cold War, had the Northern Territory as its theatre of representation. This book explores a period that saw a federal experiment to normalise the north, the black half of a White Australia, across a vast geographic region with diverse population; the results are often surprising and offer new insight into this period in Australian history. The editors are three historians with a wide experience of researching and writing Territory history. Modern Frontier provided them an exciting opportunity to work with a range of authors representing different disciplines and perspectives, on a subject where the issues still powerfully resonate today, more than half a century on. [ ...A multitude of facets in Territory affairs a half-century ago. It's a new view... a rich, often provocative one. Professor Alan Powell, author of 'Far Country'.]Section A: White dreams, white schemes: assimilating the territory. Ch. 1. Finding the road to Rum Jungle / Julie T. Wells -- Ch. 2. Nation and assimilation: continuity and discontinuity in Aboriginal affairs in the 1950s / Russell McGregor -- Section B: Settlers and settling. Ch. 4. Health: a matter of control / Suzanne Parry -- Ch. 5. You are what you eat: food and cultural identity / Mickey Dewar -- Ch. 6. From 'native relics to Flynn's pillar': cultural heritage management in the Northern Territory during the 1950s / David Carment, Clayton Fredericksen and Kathy De La Rue -- Ch. 7. Populating the Northern Territory / Margaret Landigran and Julie T. Wells -- Section C: What's yours is mine ... Ch. 8. Crossing trajectories: the Northern Territory and Australian art / Daena Murray -- Ch. 9. Corroboree moderne / Suzanne Spunner -- Ch. 10. The art of engagement: indigenous art and outside influence / Margie West -- Appendix A -- Appendix B -- IndexJira Ticket : CDU-290 : Collection Development Manager made the decision that for the books that have this message "
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Proposta di traduzione e commento del romanzo "'Til All These Things Be Done" di Suzanne Moyers.
No full textL'obiettivo di questa tesi è quello di fornire una proposta di traduzione letteraria dall'inglese all'italiano corredata di un'analisi delle strategie traduttive adottate. L'elaborato si divide in tre capitoli: nel primo viene presentata l'autrice, Suzanne Moyers, e l'opera, 'Til All These Things Be Done, con particolare attenzione al genere e alla funzione del testo, al lettore modello a cui è rivolto e alla particolare varietà linguistica di anglo-americano impiegata dall’autrice; nel secondo, alla proposta di traduzione segue una breve esplicitazione della macrostrategia traduttiva. Si procede dunque a un’analisi comparata dei fenomeni linguistici più rappresentativi del source text con la resa nel target text; infine, si traggono le conclusioni del lavoro svolto ponendo particolare attenzione a come l’italiano ha risposto alle richieste stilistiche e linguistiche dell’originale ai vari livelli testuali (fonologico, morfologico, sintattico e lessicale).
This thesis is an example of literary translation from English to Italian. The book, ‘Till All These Things Be Done is written by Suzanne Moyers. My study is divided into three sections. Chapter I introduces the author and her work, mainly emphasizing on the genre and the function of the text, the reader to whom it is addressed, and the particular linguistic variety of American English chosen by the author. Chapter II puts the stress on my translation proposal, on the macro-strategy used during the rendering process and, last but not least, on the comparative analysis of the most representative linguistic phenomena both in the source and the target text. To conclude, I draw conclusions from the work done, paying particular attention to how Italian responded to the stylistic and linguistic demands of the original at the various textual levels (phonological, morphological, syntactic, and lexical)
Isolation and characterization of lipid rafts in Emiliania huxley: key players in host-virus interactions
No full textCoccolithoviruses (EhVs) employ a suite of glycosphingolipids (GSLs) to successfully infect the globally important coccolithophore Emiliania huxleyi. Given their enrichment in glycosphingolipids and their involvement in sensing extracellular stimuli and activating signaling cascades through protein-protein interactions, lipid rafts and associated microdomains likely play a heretofore unappreciated role in viral infectivity and resistance mechanisms. Indeed, the lipid raft protein flotillin carries an evolutionary conserved prohibitin homology (PHB) domain, which has been shown to influence pathogen resistance by inducing hypersensitive response and cell death. Little to nothing is known about lipid rafts in unicellular photoautotrophic organisms, especially those from the marine environment. We combined bioinformatics, detergent treatment, density gradient purification, western blotting, lipidomics and genome-enabled proteomics to isolate and characterize the lipid and protein content of lipid rafts from control and EhV86-infected E. huxleyi strain CCMP1516. Using bioinformatic analysis, we identified a flotillin-like protein (Protein ID 363433) in the E. huxleyi CCMP1516 genome, which contained several evolutionary-conserved features of known flotillin proteins. Western blot analysis further revealed immunoreactivity of a 67 kDa protein in E. huxleyi cell extracts to a human anti-flotillin antibody, consistent with the dimerized form of this protein and thereby providing a biochemical marker for lipid raft isolation. Lipid raft-enriched fractions were isolated and purified as detergent (Brij96) resistant membranes (DRMs) in Optiprep density gradients and their lipid and protein composition were subsequently characterized. TSQ mass spectrometry of the lipids in DRM fractions showed novel host-derived GSLs which co-purified with flotillin immunoreactive fractions. These fractions also contained notable vesicle morphology as confirmed by TEM. Subsequent analysis of lipid raft proteome in both uninfected and EhV86-infected E. huxleyi cells collected at 2 h confirmed flotillin as one of the major proteins and more broadly provided key insight into host defense and virus infection strategies at the interface of lipid rafts and stress, programmed cell death, and innate immunity pathways. In particular, the detection of an EhV86-encoded C-type lectin (CTL)-containing protein (ehv149), with putative roles in cell adhesion and pathogen recognition receptors, suggests that EhV86 infection of E. huxleyi occurs by way of the recognition of specific glycosphingolipids and raft-associated proteins.M.S.Includes bibliographical referencesby Suzanne L. Ros
ANALYSIS OF MORAL AND CULTURAL VALUES IN THE HUNGER GAMES BY SUZANNE COLLINS
No full textFilm is one of the media frequently used by many people. This research aims to utilize films as a learning medium for students who want to understand foreign cultures, gain advanced literacy skills in English, and learn correct pronunciation. The Hunger Games by Suzanne Collins contains moral messages and cultural values. In this research, the author discusses the moral messages according to Suseno's theory and the cultural values according to Koentjaraningrat's theory contained in the film, and analyzes them using a qualitative descriptive method by explaining data derived from film excerpts
Design and synthesis of G-quadruplex selective macrocyclic polyoxazoles
No full textNucleic acid sequences that can form stable G-quadruplex structures have been implicated in numerous biological functions. G-quadruplexes are thought to play a vital role in telomere maintenance and regulating gene expression. The majority of cancer cells over-express telomerase, an enzyme that bypasses the aging process by adding telomeric repeats to chromosome ends. Also about 65% of oncogenes have a potential G-quadruplex forming sequence in their promoter region. It is believed that stabilized G-quadruplexes can indirectly inhibit telomerase and decrease oncogene expression resulting in cancer cell death. These observations have led to the development of small molecules that stabilize G-quadruplexes as a new class of anticancer agents. Several research groups have developed such molecules but most lack selectivity over other forms of DNA. A unique molecule among these is telomestatin, a macrocyclic natural product with 70-fold selectivity for G-quadruplex DNA over duplex and low micromolar cytotoxicity. Although telomestatin has exceptional activity, it is difficult to synthesize and hard to formulate because it lacks water-solubilizing groups. Initial studies utilized telomestatin as a lead structure to develop a novel class of macrocyclic hexaoxazoles with the most active compound being HXDV. It was shown to stabilize G-quadruplex DNA with no detectable interaction with duplex DNA and had comparable cytotoxic activity to telomestatin. Unfortunately, HXDV had limited aqueous solubility and required a lengthy synthesis. Efforts to modify the structure of HXDV to enhance water solubility and simplify the synthetic process are described in this dissertation. While this was generally successful, a shorter synthesis was needed to rapidly assess structure-activity relationships. This led to the development of a second class of compounds called macrocyclic pyridyl polyoxazoles (PyPX). These compounds utilize a molecular scaffold that is readily accessed in few synthetic steps to form numerous macrocyclic analogs. This enables quick assessment of structure-activity relationships. Several members of this class are excellent G-quadruplex stabilizers and do not interact with duplex DNA. These compounds also possess low nanomolar cytotoxicity and one was active in an in vivo bioassay. Structure-activity studies utilizing the PyPX scaffold continue in an effort to identify clinical candidates.Ph.D.Includes bibliographical referencesIncludes vitaby Suzanne G. Rzucze
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