1,164 research outputs found
Giulia Veronica Varisco
No full textThe headword explains the biography and the contribution of the author Giulia Varisco to the children's literatur
Potenziamento dell’efficacia del cetuximab mediante combinazione con cellule T ingegnerizzate con recettori Fc chimerici per il trattamento del carcinoma del colon retto K-ras mutato.
No full textINTRODUZIONE: L’impiego di anticorpi monoclonali (AM) diretti contro il recettore per il fattore di crescita epidermico (EGFR), tra cui il cetuximab, ha rappresentato un significativo avanzamento nel trattamento del carcinoma colorettale metastatico (mCRC). L’effetto anti-tumorale del cetuximab è dovuto a: i) blocco della proliferazione neoplastica; ii) attivazione di processi apoptotici; iii) induzione di citotossicità cellulare dipendente da anticorpo (ADCC) mediante il legame con i recettori Fc. Tuttavia, diversi fattori limitano l’efficacia terapeutica del cetuximab; tra questi la presenza di mutazioni nel gene Kras (Kras-mut) e la scarsa presenza di cellule Natural Killer, capaci di mediare ADCC, nel microambiente tumorale. In questo studio ipotizziamo di superare i limiti terapeutici del cetuximab attraverso la sua combinazione con cellule T ingegnerizzate con recettori chimerici Fc (Fc-CRs).
MATERIALI E METODI: Due Fc-CRs, denominati CD32-CR e CD16-CR, sono stati generati dalla fusione della regione extracellulare del FcRIIA (CD32) o del FcRIIIA (CD16) con il dominio transmembrana del CD8a e con i domini intracitoplasmatici del CD28 e del CD3. Linfociti T attivati sono stati trasdotti con le chimere e la corretta espressione è stata valutata mediante citofluorimetria e western blot. I recettori sono stati caratterizzati funzionalmente in vitro attraverso test di binding e saggi ELISA. L’attività anti-tumorale delle cellule T trasdotte in associazione al cetuximab è stata valutata in test di deplezione in vitro e in topi SCID xenotrapiantati con la linea cellulare di CRC Kras-mut HCT116.
RISULTATI: Entrambi i Fc-CRs sono espressi correttamente sulla superficie delle cellule T. Il CD32-CR, ma non il CD16-CR, lega in maniera specifica il frammento Fc degli AM anti-EGFR cetuximab e panitumumab. La dose massima di cetuximab necessaria per saturare tutti i CD32-CR che è pari a 10g/ml ed il binding è mantenuto anche in presenza di quantità crescenti di plasma umano. Sia il CD32-CR che il CD16-CR inducono rilascio di citochine in risposta a stimolazione con cetuximab o panitumumab. La combinazione del cetuximab con cellule T CD32-CR+: i) induce l’eliminazione di HCT116 in vitro; ii) riduce significativamente la crescita tumorale in vivo.
CONCLUSIONI: In questo studio preclinico dimostriamo che cellule T armate con CD32-CR potenziano l’efficacia del cetuximab contro CRC Kras-mutato
Ytterbium Disilicate/Monosilicate Multilayer Environmental Barrier Coatings: Influence of Atmospheric Plasma Spray Parameters on Composition and Microstructure
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Ytterbium Disilicate/Monosilicate Multilayer Environmental Barrier Coatings: Influence of Atmospheric Plasma Spray Parameters on Composition and Microstructure
by Giulia Di Iorio,Laura Paglia *ORCID,Giulia PedrizzettiORCID,Virgilio GenovaORCID,Francesco MarraORCID,Cecilia BartuliORCID andGiovanni PulciORCID
INSTM Reference Laboratory for Materials and Surface Engineering, Sapienza University of Rome, Eudossiana 18, 00184 Rome, Italy
*
Author to whom correspondence should be addressed.
Coatings 2023, 13(9), 1602; https://doi.org/10.3390/coatings13091602
Original submission received: 10 August 2023 / Revised: 31 August 2023 / Accepted: 11 September 2023 / Published: 13 September 2023
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Abstract
SiC/SiC ceramic matrix composites (SiCf/SiC CMCs) are regarded as the new materials for the hot-section components of aircraft gas turbine engines, since they have one-third of the density of metallic superalloys, a higher temperature capability, good mechanical strength, and excellent thermal shock resistance. However, high-temperature water-vapor-rich combustion gases can induce severe surface recession phenomena in SiC/SiC leading to component failure. For this reason, it is necessary to design protective coatings, i.e., environmental barrier coatings (EBCs), able to protect the SiC/SiC surface in combustion environments. In the present work, ytterbium monosilicate (Yb2SiO5), stable when exposed to water vapor at high temperatures, and ytterbium disilicate (Yb2Si2O7), characterized by a thermal expansion coefficient closer to that of the substrate, were selected for a multilayer EBC system. EBCs were processed using the atmospheric plasma spray (APS) technique. A set of deposition parameters were tested, varying the power of the torch, and the composition and microstructure of the deposited coatings were studied in terms of porosity, crack density, and post-deposition phase retention by performing SEM, EDS, and XRD analysis. The results allow for the definition of the influence of deposition parameters on the final properties of multilayer EBC coatings
HSP70 production and inhibition of cell proliferation in molt-4 T-cells after cell-to-cell transmission of HTLV-I: Effect of PGA1
No full textInfection with HTLV-I is associated with leukemic transformation of mature CD4(+) T lymphocytes. PGA(1), a powerful inhibitor of tumour cell proliferation, can prevent the clonal expansion of HTLV-I-infected cells following acute infection of cord blood-derived mononuclear cells. Since the antiproliferative effect of PGA(1) on HTLV-I transformed, chronically infected MT-2 cell line was associated with induction of HSP70, we have investigated the effect of PGA(1) on cell cycle progression and HSP70 production in a leukemic T-cell line (Molt-4) shortly after exposure to HTLV-I in a cell-to-cell transmission model. rate of cell proliferation and HSP70 expression were studied within one duplication cycle of Molt-4 cells after exposure to HTLV-I. growth of both control and virus-exposed cultures was inhibited by treatment with PGA(1) (4 mu g/ml) and cell cycling was arrested preferentially at the G(1)/S interphase. synthesis of HSP70 was induced within 3 h by PGA(1) in control and virus-exposed Molt-4 cells and became undetectable from overnight onward, though the protein accumulated in the cells. the arrest of growth was observed from overnight up to 48 h so that treated cells almost missed one cycle. Interestingly, HSP70 transcript and protein persisted at remarkably high levels in molt-4 cells exposed to HTLV-I in the absence of PGA(1), showing that HSP70 expression can be directly activated during primary infection with this human retrovirus. moreover, in these cocultures, treatment with PGA(1) or heat shock was not able to increase further the elevated level of HSP70 found in untreated cocultures, suggesting that during the early period of the virus-transmission phase, HTLV-I could interfere with HSP70 induction by other inducers
Correction to: Safety and Psychological Outcomes of Tandem t:Slim X2 Insulin Pump with Control-IQ Technology in Children, Adolescents, and Young Adults with Type 1 Diabetes: A Systematic Review
No full textThe second author name was incorrectly published as Giula Maria Smylie. The correct name is Giulia Marie Smylie. The original article has been corrected
Scrivere senza anestesia. La chiarezza di Giulia Niccolai
No full textIl saggio colloca storicamente la narratrice e poetessa Giulia Niccolai nel canone del Novecento letterario italiano discutendone poetica e cifre stilistiche. L'ampia analisi proposta tocca tutte le opere dell'autrice evidenziandone i legami intertestuali, anche tra poesia e narrativa, e i progressivi sviluppi in un arco cronologico esteso, tra anni Sessanta e primi anni Duemila. Lo studio coglie anche l'importanza dei riferimenti alle arti visive, in particolare alla fotografia, che Giulia Niccolai ha praticato in prima persona negli anni della Neoavanguardia, e alla pittura americana.The essay places the narrator and poet Giulia Niccolai in the canonical twentieth century Italian literary discussing her poetics and stylistic figures. The wide analysis proposed touches all the works of the author highlighting the intertextual links, also between poetry and narrative, and the progressive developments in an extended chronological period, between the Sixties and early Twenties. The study also captures the importance of references to the visual arts, especially photography, which Giulia Niccolai has practiced in the years of the Neo-avant-garde, and to American painting
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L'afrofuturismo tra Stati Uniti e Italia. Dalla memoria storica ai viaggi intergalattici per re-immaginare futuri postumani
No full textIn this article the author explores the cultural movement called Afrofuturism, tracing its origins, features, simbology, and its historical and political meanings. Starting from the (supposed) discoursive gap between African and African American people on one side and technology and science fiction on the other, this paradigm addresses themes related to the intersection of African Diaspora and African cultures with technology and science fiction, with the aim to recover the history of slavery and racism through the projections into alternative space-time contexts. In particular, the author explores the feminist afrofuturism perspective in order to examine the specific oppression and cultural production of African and African American women. In doing so, the author analyzes the development of afrofuturism in Italy, focusing on the work of the singer and beatmaker Karima 2G
BDC-Decomposition for global influence analysis
No full textIn biochemical networks, the steady-state input-output influence is the sign of the output steady-state variation due to a persistent positive input perturbation; if the sign does not depend on the value of the strictly positive system parameters, the influence is structural. As recently shown for small perturbations, when the linearized system approximation is valid, steady-state input-output influences can be structurally assessed, for biochemical networks with m unknown parameters, by means of a vertex algorithm with complexity 2m. This letter shows that the structural input-output influence of a biochemical network is a global property, which does not require any small-perturbation assumption. It also shows that, using a new algorithm, the complexity can be reduced down to 2m-n , where n is the system order, thus drastically reducing the computation time. Finally, when the uncertain parameters belong to known intervals, non-conservative bounds are given for the steady-state ratio between output and input, allowing for sensitivity analysis.Accepted Author ManuscriptTeam Tamas Keviczk
Luoghi di danza
No full textWriter, journalist, theater and film critic, Marco Ramperti is one of the few that deals with dance in the Italian press of the fascist period. For the newspaper «La Stampa», between 1926 and 1928, he edits the column Luoghi di danza (dance venues), dedicated to ballroom and modern dances. The article investigates the performative nature of Ramperti’s writing, dominated by “staging” devices of the subject matter, as well as by an intrusive presence of the body, first of all that of the author. What emerges is an unprecedented image of the dance in the Twenties, at the same time catalysing the most alienating - and Pirandellian - aspects of modernity, but also a place where genuinely experiencing themselves and the others
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