11 research outputs found
Dynamic analysis of rotating fan structures by the Finite Element Method
No full textThe Finite Element Method is used to determine the fundamental mode shapes and frequencies of fan structures. The analysis is performed on five fan structures, having different geometries, under static conditions and under centrifugal forces at various angular velocities. The results of the analysis are correlated with experimental data. The Southwell Coefficient, which takes into account the effect of centrifugal stiffening/softening, is determined for each fan and used for the prediction of its dynamic response and critical speeds. The research led to a study of the influence of riveted spider-to-blade connections on the vibration response of propeller fans; This thesis also presents a mathematical transformation analysis and an explanation of a frontend computer program, to the finite element system GIFTS, which facilitates the procedure of analyzing the vibration response of propeller fans under static or operating conditions, by the Finite Element Method. (Abstract shortened with permission of author.)
Breast Cancer Molecular Subtypes Among Moroccan Women
No full textIntroduction: Breast cancer remains despite the therapeutic progress, the leading cause of death by cancer among women. It represents a group of very heterogeneous clinical, histopathological and molecular diseases. Molecular heterogeneity has been demonstrated by genomic analysis, even for similar histology cancers. Four subgroups of breast carcinomas are distinguished: Luminal A, Luminal B, HER2 over expression and Basal - like. The Immuno-histo-chemical analysis useip (estrogen receptors) RE, the PR (progesterone receptors), the ((Human Epidermal Growth Factor Receptor-2), the Ki67 (proliferation marker) HER2, CK5/6) has shown a subdivision into subgroups similar to those found by genomic analysis. These subgroups are different from the point of view of clinical course and response to adjuvant treatment.
Objectives: The aim of this work is to study the molecular profile of the breast cancers by immunostaining on Moroccan series to a classification with a prognostic value allowing a treatment tailored to each group of patients. Furthermore, the molecular subgroups were correlated to other clinical and histological factors.
Material and methods: It is a prospective study of the laboratory of Anatomy and Pathologic cytology of the children's Hospital, the service I of the maternity hospital in Rabat and in cooperation with the United Nations Centre of pathological anatomy. To do this, 88 cases of breast cancer together were diagnosed between January 1, 2010 and December 31, 2014, taking a period of five years. All tissue samples made subject study of Immuno-histo-chemistry with the following markers: RE, PR, HER2 and Ki67. Only negative triple cases (HR and HER2 negative) benefited from an additional marking with CK5/6 and EGFR to set the basal profile.
Results: Series of 88 cases of mammary carcinomas observed on operating parts, ranged in age between 28 and 84 years old, with an average of 51 ± 12, 8. Carcinoma infiltrating non-specific (DOCTORS) was the most frequent (87.5%). Ranks histo-prognostic Scarff Bloom and Richardson (SBR) 2 and 3 respectively accounted for 45.5 and 51.1% of cases and only 2, 3% of the DOCTORS were grade 1. The Luminal B (53.4%) was under the most common molecular group, followed by Luminal A (23.9%), HER2 + (15.9%) and triple negative (6.8%). The correlation of molecular type of tumors with different prognostic factors showed only one significant connection with the SBR grade.</jats:p
Correction to: An update of the aetiological factors involved in molar incisor hypomineralisation (MIH): a systematic review and meta-analysis (European Archives of Paediatric Dentistry, (2022), 23, 1, (23-38), 10.1007/s40368-021-00646-x)
No full text\ua9 The Author(s), under exclusive licence to European Academy of Paediatric Dentistry 2025. Correction to: European Archives of Paediatric Dentistry (2022) 23:23–38 https://doi.org/10.1007/s40368-021-00646-x Some odds ratios and two figures were incorrectly transferred from the supplementary material to the abstract and the text. The figure at the bottom of page 25 of the supplementary material has also been modified. These changes do not alter the interpretation of the results and do not affect the conclusions of the publication. The correct text including the corrected odds ratios is presented below: Perinatal factors: Prematurity (OR = 1.59; 95% CI 1.25–2.04; p = 0.0002) and caesarean delivery (OR = 1.13; 95% CI 1.01,1.28; p = 0.04) are associated with an increased risk of developing MIH. Birth complications are also highlighted. These three factors can lead to hypoxia, and children with perinatal hypoxia are more likely to develop MIH (OR = 2.59; 95% CI 1.69–3.98; p < 0.0001). Following the exclusion of these studies, hypoxia at birth (OR = 2.59; 95% CI 1.69–3.98; p < 0.0001; Fig. 4), caesarean (OR = 1.13; 95% CI 1.01,1.28; p = 0.04; Fig. 5) and prematurity (OR = 1.59; 95% CI 1.25–2.04; p = 0.0002; Fig. 6) were associated with MIH (Table 2). (Figure presented.) (Figure presented.) Meta-analysis (forest plot and funnel plot) for caesarean Meta-analysis (forest plot and funnel plot) for prematurity After excluding outlying studies that severely increased heterogeneity, factors such as gastric disorders (OR = 1.99; 95% CI 1.37–2.88; p = 0.0003), fever (OR = 1.44; 95% CI 1.19–1.75; p = 0.0002), kidney diseases (OR = 2.70; 95% CI 1.07–6.82; p = 0.04), pneumonia (OR = 1.49; 95% CI 1.15–1.94; p = 0.03), asthma (OR = 1.56; 95% CI 1.10–2.20; p = 0.01) and antibiotic use (OR = 1.20; 95% CI 1.01–1.44; p = 0.04) were also found to be associated with MIH (Tables 2, 3). It was finally determined that prematurity is associated with MIH (It was finally determined that prematurity is associated with MIH (OR = 1.45; 95% CI 1.24–1.70; p = 0.0002). Additionally, Table 3, Figs. 5 and 6 and the bottom figure in Supplementary materials p25 are replaced and included in the present correction as supplementary material. Finally, in the publication ‘Lygidakis NA, Garot E, Somani C, Taylor GD, Rouas P, Wong FSL. Best clinical practice guidance for clinicians dealing with children presenting with molar-incisor-hypomineralisation (MIH): an updated European Academy of Paediatric Dentistry policy document. Eur Arch Paediatr Dent. 2022;23(1):3–21, the following odds ratios were affected and the correct ones are presented below, again not affecting the results interpretation and Conclusions: Few of the above-mentioned discrepancies affect also minimally the publication ‘Lygidakis NA, Garot E, Somani C, et al. Best clinical practice guidance for clinicians dealing with children presenting withmolar-incisor-hypomineralisation (MIH): an updated European Academy of Paediatric Dentistry policy document. Eur Arch Paediatr Dent. 2022;23(1):3–21, but again do not alter the results interpretation and do not affect the conclusions of the publication. (Table presented.) Meta-analyses with high heterogeneity Aetiologies Studies causing high I2 n study I2 Chi2p value OR [95% C] p value Gastric disordersa Koruyucu et al. (2018) Incubatora Andrade et al. (2017) 4 0% 0.62 0.94 [0.71, 1.23] 0.64 Maternal fever None – – – – – Ghanim et al. (2013); de Deus Moura de Lima et al. (2015) Kidney diseasesa Ahmadi et al. (2012) Ghanim et al. (2013) Ahmadi et al. (2012); Allazzam et al. (2014) Sidaly et al. (2016); Teixeira et al. (2018) < 0.0001* Mejia et al. (2019); Ahmadi et al. (2012) Ghanim et al. (2013); Ahmadi et al. (2012); Allazzam et al. (2014) Tonsillitis None – – – – – Diarrhoeaa Koruyucu et al. (2018) 6 0% 0.52 1.01 [0.81, 1.27] 0.92 Lygidakis et al. (2008); Ahmadi et al. (2012); Dantas-Neta et al. (2018) Chicken pox None – – – – – Asthma druga Tourino et al. (2016) 4 0% 0.66 0.95 [0.77, 1.16] 0.59 Low weight at birth Ghanim et al. (2013) 10 30% 0.17 1.09 [0.86, 1.39] 0.47 Breast feeding > 6 months Ghanim et al. (2013) 11 35% 0.12 1.01 [0.85, 1.19] 0.94 aThe meta-analysis was performed with less than 10 studies The correct text including the corrected odds ratios is presented below: substantially increased the possibility of having MIH (OR = 2.59; 95% CI 1.69–3.98; p < 0.0001 (Garot et al. 2021). prematurity was significantly associated with MIH (OR = 1.59; 95% CI 1.25–2.04; p = 0.0002) (Garot et al. 2021). caesarean section increased the possibility of having MIH (OR = 1.13; 95% CI 1.01,1.28; p = 0.04)
Antioxidant status in rat kidneys after coexposure to uranium and gentamicin
No full textInternational audienceUranium (U) accumulates and produces its toxic effects preferentially in the kidneys, especially in the proximal tubular structure. U disturbs the balance of pro-/antioxidants in the renal cortex after acute exposure. Other nephrotoxic agents, such as medications, also cause oxidative stress, but the effects of coexposure are not known. The aim of this study was to analyze the effect of chronic exposure to U and acute gentamicin treatment on the pro- and antioxidant status of the renal cortex of rats. Animals were chronically exposed (9 months) to a nonnephrotoxic level of U (40 mg/L) and then treated with daily injections of gentamicin at a range of doses (0, 5, 25, 100, and 150 mg/kg) during the last week of contamination. We studied changes in the gene expression, protein expression, and enzyme activity of key factors involved in the pro-/antioxidant balance in the renal cortex. At and above a dose of 100 mg/kg, gentamicin decreased the messenger RNA (mRNA) levels of catalase (CAT), copper/zinc superoxide dismutase (SOD) and increased the mRNA levels of heme oxygenase-1 in contaminated rats. This treatment decreased CAT activity, but did not significantly change the SOD protein level. Chronic exposure to U did not worsen these effects in our experimental conditions. In conclusion, gentamicin treatment disturbed the oxidative balance in our model's renal cortex, but the chronic exposure to U at this nonnephrotoxic level did not appear to reinforce these effects. © The Author(s) 2013
An update of treatment modalities in children and adolescents with teeth affected by molar incisor hypomineralisation (MIH): a systematic review
No full text\ua9 2021, The Author(s).Purpose: To systematically review the treatment modalities for molar-incisor hypomineralisation for children under the age of 18 years. The research question was, ‘What are the treatment options for teeth in children affected by molar incisor hypomineralisation?’ Methods: An electronic search of the following electronic databases was completed MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, LILACS, Google Scholar and Open Grey identifying studies from 1980 to 2020. The PRISMA guidelines were followed. The studies were screened, data extracted and calibration was completed by two independent reviewers. Results: Of 6220 potential articles, 34 studies were included. Twenty studies investigated management of molars with fissure sealants, glass ionomer cement, polyacid modified resin composite, composite resin, amalgam, preformed metal crowns, laboratory-manufactured crowns and extractions. In four articles management of incisors with microabrasion, resin-infiltration and a combination of approaches was reported. Eight studies looked at strategies to mineralise MIH-affected teeth and/or reduce hypersensitivity. Two studies investigated patient-centred outcomes following treatment. Due to the heterogeneity between the studies, meta-analysis was not performed. Conclusion: The use of resin-based fissure sealants, preformed metal crowns, direct composite resin restorations and laboratory-made restorations can be recommended for MIH-affected molars. There is insufficient evidence to support specific approaches for the management of affected incisors. Products containing CPP-ACP may be beneficial for MIH-affected teeth
Phytochemical composition and toxicity assessment of Ammi majus L.
No full textObjective: To assess the acute and subacute toxicity as well as the phytochemical composition of two extracts and three fractions of Ammi majus L.
Methods: The aqueous extracts were prepared separately by maceration for 48 h and by infusion for 1 h, while the fractions were prepared by the Soxhlet extractor, successively employing cyclohexane, ethyl acetate, and ethanol. The acute toxicity study was carried out in accordance with the OECD N°423 guideline at a single dose (2000 mg/kg) in mice for 14 days. The subacute toxicity study was performed by a daily oral administration of 250 mg/kg for 10 days and 100 mg/kg doses for 28 days. Phytochemical screening was performed using staining and precipitation reactions, while the chemical characterization of some analytes was detected by HPLC-MS/MS analysis.
Results: In the acute toxicity study, no signs of toxicity such as convulsion, salivation, diarrhea, sleep and coma were observed during 30 minutes and 14 days, so the lethal dose was higher than 2000 mg/kg for each extract and fraction. The subacute toxicity results showed that at a dose of 250 mg/kg, 61.10% of the animals died and the rest developed morbidity. On the other hand, at a dose of 100 mg/kg, all the animals were still alive after 28 days, with no morbidity and the biochemical parameters were normal with no abnormalities in the liver, kidneys and pancreas. Phytochemical screening indicated the presence of flavonoids, tannins, coumarins, and free quinones and the absence of alkaloids and anthocyanins.
Conclusions: The extracts and fractions of Ammi majus L. are not toxic in the short and long term with a varied chemical composition. Toxicological tests on animals other than rodents and in the long term (more than 28 days) are needed to further confirm the safety of Ammi majus extracts
Extranodal T/NK-cell lymphoma with fronto-orbital localization: A case report
No full textOrbito-cerebral lymphoma in children is a rare cancer affecting the orbital and cerebral regions, often associated with extranodal lymphomas. It typically presents with ocular, neurological, and systemic symptoms, which can mimic other conditions, delaying diagnosis. MRI is the primary imaging tool for diagnosis. Treatment involves chemotherapy, sometimes with radiotherapy, and newer targeted therapies have improved survival. The prognosis is favorable for localized disease, but advanced cases or brain involvement have a higher risk of recurrence. Regular follow-up is essential to monitor for recurrence and treatment side effects
Heterozygous MEFV Mutation Leading to Renal Failure: A Case Study
No full textFamilial Mediterranean fever (FMF) is an autosomal recessive disorder, particularly common in the Mediterranean area. Mutations in the MEVF gene cause it. AA Amyloidosis is the most severe complication of FMF leading to chronic renal failure. We describe a rare pediatric case of a phenotype I familial Mediterranean fever with V726A heterozygous mutation. The diagnosis was made at chronic kidney disease. We discuss through this case the importance of the early diagnosis of FMF heterozygous children which is not usually evident in some phenotypes. It will surely avoid fatal complications, inappropriate therapeutic approaches and higher healthcare costs
Acute and Subacute Toxicity Studies of Erodium guttatum Extracts by Oral Administration in Rodents
No full textThe present study aimed to evaluate the acute and subacute toxicity profiles of Erodium guttatum extracts in mice using the methods described in the guidelines of the OECD. In the acute toxicity study, the LD50 value was greater than 2000 mg/kg. The subacute toxicity study of E. guttatum extracts showed no significant changes in body or organ weights. The administration of E. guttatum extracts to mice at a dose of 200 mg/kg led to an increase in white blood cells, platelets and hemoglobin. Moreover, the aqueous extract of E. guttatum only decreased liver aspartate aminotransferase (ASAT) levels at a dose of 200 mg/kg, and creatinine and urea levels did not show any significant alterations compared to the control group. Our results showed that the extracts of E. guttatum caused a slight increase in alanine aminotransferase (ALAT) and triglycerides. The histological study showed that mice treated with E. guttatum extracts experienced some histopathological changes in the liver, particularly with the methanolic extract, and slight changes in the kidneys and pancreas. Regarding the renal profile, no toxicity was observed. These results provide basic information on the toxicological profile of E. guttatum used in traditional medicine
Exploring the Role of Topoisomerase II Beta in Macrophage Maturation and Pro-inflammatory Cytokine Production
No full textAlthough it is known that DNA topo IIβis required for the regulation of transcription during neural development and differentiation, it is not clear whether the enzyme is required during differentiation of human monocytes into macrophages and/or the subsequent transcription of
cytokine genes. To test this, a robust model of differentiation of monocyte-like cells into macrophage-like cells using U937 and HL-60 cells treated with phorbol 12-myristate 13-acetate (PMA) and Lipopolysaccharide (LPS) was validated. Differentiation was determined by morphological and growth characteristics and CD11b surface antigen expression as determined by flow cytometry. qRT-PCR was also used to measure mRNA transcript levels of key genes known to be up-regulated during monocyte differentiation and the secretion of pro-inflammatory cytokines produced by differentiated cells were measured using ELISA.
siRNA topo IIβknockdown did not hinder monocyte-like cells from undergoing differentiation, however experiments revealed a correlation between topo IIβknockdown and secreted TNFα, with the latter decreasing when topo IIβwas reduced. This pattern was also noted when measuring IL-1βsecretion. Similar results were seen using a Murine transgenic fibroblast cell line lacking topo IIβ, which when stimulated with LPS secreted significantly lower levels of IL-6 compared to the wild type cells. Thus topo IIβexpression is necessary for
secretion of normal levels of the cytokines, TNFα, IL-1βand IL-6 in response to LPS at certain time points. In addition in the macrophage-like state of the two cell lines, the relative levels of the βisoform (mRNA and protein) were shown to be significantly increased
compared to α, further outlining the importance of topo IIβin the differentiated state. Chromatin immuno-precipitation followed by qPCR showed however that topo IIβwas not associated at three defined proximal promoter regions of either the TNFαand IL-1βgenes, although further studies are required to rule out a direct association of topo IIβwith these and other regions of the genes.
Down regulation of topo IIβprotein using the inhibitor ICRF-193 did not hinder monocyte-like cells from undergoing differentiation either. However, contrary to the knockdown results, a 6 h pre-treatment with 1 nM ICRF-193 increased TNFαlevels in these cells, both at the
mRNA and the protein level, along with a slight increase in secreted TNFα. NF-κB, EGR2, TLR4 and TLR2 transcript levels were also increased under these conditions. Thus further studies are required to determine if these increases are due to additional cellular effects of the
drug or whether topo IIβmay play an inhibitory effect on transcription.
Thus it is clear that topo IIβplays an important role in expression of cytokines and understanding the exact nature of this requires further research that may yield potential new avenues for treatment of disease
