1,720,971 research outputs found
Ruolo del prodotto del gene ilvIH sull’attività dell’isozima acetoidrossi acido sintasi III di E. coli K-12.
No full textUtilization of cellobiose and other β-D-glucosides in Agrobacterium tumefaciens
No full textAgrobacterium tumefaciens strain C58 was able to utilize carbon from cellobiose and some other β-D-glucosides as efficiently as from glucose. β-D-glucoside utilization was partially inducible and the induction was subject to catabolite repression by glucose, independently of the presence of cyclic AMP in the medium. It was also independent of Ti plasmid-encoded functions. β-D-glucosides were hydrolysed by a single, cytoplasmic and constitutively expressed β-glucosidase, which was active on non-phosphorylated substrates and insensitive to glucose inhibition. © 1995
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Structure-function analysis of the EGF-CFC family member Cripto identifies residues essential for nodal signalling
No full textCripto is the founding member of the family of EGF-CFC genes, a class of extracellular factors essential for early vertebrate development. In this study we show that injection of Cripto recombinant protein in mid to late zebrafish Maternal-Zygotic one-eyed pinhead (MZoep) blastulae was able to fully rescue the mutant phenotype, thus providing the first direct evidence that Cripto activity can be added extracellularly to recover oep-encoded function in zebrafish early embryos. Moreover, 15 point mutations and two deletion mutants were generated to assess in vivo their functional relevance by comparing the ability of cripto wild-type and mutant RNAs to rescue the zebrafish MZoep mutant. From this study we concluded that the EGF-CFC domain is sufficient for Cripto biological activity and identified ten point mutations with a functional defective phenotype, two of which, located in the EGF-like domain, correspond to loss-of-function mutations. Finally, we have developed a three-dimensional structural model of Cripto protein and used it as a guide to predict amino acid residues potentially implicated in protein-protein interaction
The ilvIH operon of E. coli K-12. Identification of the gene products and recognition of the translational start by polypeptide micro-sequencing
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