104,542 research outputs found
Pine & Lafferty (1982) C2H6 spectral data
No full textC2H6 spectral data from: Pine, A.S & Lafferty, W.J.,
"Torsional Splittings and Assignments of the Doppler-Limited
Spectrum of Ethane in the C-H Stretching Region",
Journal of Research of the National Bureau of Standards, Vol. 87. No. 3, May-June, 1982.
These data were available only under the form of a table in the PDF copy of the original paper. The data have been read and transcripted to a single ASCII text file by the authors of Cours, T., Cordier, D., Seignovert, B., Maltagliati, L., Biennier, L., "The 3.4 um absorption of the Titan's stratosphere: contribution of ethane, propane, butane and complex hydrogenated", 2020, Icarus, 339, 113571. DOI: 10.1016/j.icarus.2019.113571. These spectral lines are now much more usable (at least for comparison purpose) by researchers.</p
Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose
No full textFriehs K, Lafferty RM. Cloning and expression of the levanase gene in Alcaligenes eutrophus H16 enables the strain to hydrolyze sucrose. Journal of Biotechnology. 1989;10(3-4):285-291.Genetic engineering methods were used to enhance the substrate spectrum of Alcaligenes eutrophus H16, a poly-[beta]-hydroxybutyric acid (PHB) producer. Using parts of the vector pMMB33 and a 2.5 kb DNA fragment of the Bacillus subtilis chromosome a plasmid was constructed bearing the gene for levanase, an enzyme able to hydrolyze various saccharides. After transfer of the levanase gene by triparental conjugation, the gene, controlled by its own Bacillus subtilis promoter, is expressed in Alcaligenes eutrophus H16 and enables the strain to hydrolyze sucrose. However, growth on sucrose is limited; i.e. the sucrose is not transported efficiently into the cell and/or the levanase is not secreted into the medium
Letter, [Author unclear] to Paulina T. Merritt
No full textHandwritten letter to Paulina Merritt from an unknown author, October 1, 1876.
Lafferty baptism certificate
No full textThis item is a decorated, calligraphic, and duo-colored certificate confirming the baptism of Ecca Lafferty on May 13, 1866. The certificate states that Ecca received the Apostolical Benediction in the Rite of Confirmation by the laying on of hands by the Right Reverend Alfred Lee, D.D., and was attested by T. Gardiner Littell, the Church Rector. The document features a double black ink frame decorated with curly vines and triangular petals at the corners, as well as sections along the sides. Each corner includes a square in red ink with a quatrefoil design formed by overlapping circles or lobes. Centered at the top, between the double border, are the words: The Doctrine of Laying on of Hands. Some text within the document is printed in red ink. Within the top border, the Trinitarian invocation is printed. Below that, centered in the design, is a haloed dove in flight, wings open, diving. The names of the baptized and those officiating, the parish name, and the date of baptism are written in hand. At the bottom of the document, within the frame, is the inscription: Then laid they their hands on them, and they received the Holy Ghost. Below the bottom border, in smaller red print, reads: Published by C. M. Burns, Philadelphia
Lafferty baptism certificate
No full textThis item is a decorated, calligraphic, and duo-colored certificate confirming the baptism of Ecca Lafferty on May 13, 1866. The certificate states that Ecca received the Apostolical Benediction in the Rite of Confirmation by the laying on of hands by the Right Reverend Alfred Lee, D.D., and was attested by T. Gardiner Littell, the Church Rector. The document features a double black ink frame decorated with curly vines and triangular petals at the corners, as well as sections along the sides. Each corner includes a square in red ink with a quatrefoil design formed by overlapping circles or lobes. Centered at the top, between the double border, are the words: The Doctrine of Laying on of Hands. Some text within the document is printed in red ink. Within the top border, the Trinitarian invocation is printed. Below that, centered in the design, is a haloed dove in flight, wings open, diving. The names of the baptized and those officiating, the parish name, and the date of baptism are written in hand. At the bottom of the document, within the frame, is the inscription: Then laid they their hands on them, and they received the Holy Ghost. Below the bottom border, in smaller red print, reads: Published by C. M. Burns, Philadelphia
Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis
No full textFriehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology. 1986;3(5-6):333-341.A DNA fragment from Bacillus subtilis strain Marburg coding for the synthesis of an enzyme catalyzing sucrose hydrolysis was cloned in Escherichia coli and detected using simple direct selection of transformants growing on sucrose. Three different clones were obtained each having a 2.5 kb EcoRI-Pstl fragment in common which was shown to be sufficient to mediate growth on sucrose. This fragment was not identical with known cloned gene fragments of B. subtilis coding for the sucrose hydrolyzing enzymes sucrase and levansucrase. It could be shown that the 2.5 kb fragment codes for a third sucrose hydrolyzing enzyme, namely for levanase. In the case of E. coli this enzyme was found to be mainly intracellular; however, a small quantity was also excreted into the periplasmic space
WIDE-BAND TUNABLE DIODE LASER HETERODYNE MEASUREMENTS
No full text J. P. Sattler, T. L. Worchesky, K. J. Ritter, and W. J. Lafferty, Opt. Lett. 5, 21 (1980)Author Institution:A technique for rapid, accurate, and copious diode laser heterodyne measurements of infrared absorption frequencies will be discussed in By use of a wideband (3 dB width, 1.2 GHz) HgCdTe photomixer and a laser local oscillator, absorptions lying within 9 GHz of a emission line may be measured with care to within 6 MHz. The data from accurate infrared heterodyne measurements of 1,1-difluoroethylene, when supplemented with existing microwave data on the ground state, permit the calculation of submilimeter wave laser emission frequencies to within a few megahertz. Similar measurements on carbonyl sulfide increase its utility as a secondary frequency standard
Human beta defensin 2 selectively inhibits HIV-1 in highly permissive CCR6+CD4+ T cells
No full textChemokine receptor type 6 (CCR6)+CD4+ T cells are preferentially infected and depleted during HIV disease progression, but are preserved in non-progressors. CCR6 is expressed on a heterogeneous population of memory CD4+ T cells that are critical to mucosal immunity. Preferential infection of these cells is associated, in part, with high surface expression of CCR5, CXCR4, and α4β7. In addition, CCR6+CD4+ T cells harbor elevated levels of integrated viral DNA and high levels of proliferation markers. We have previously shown that the CCR6 ligands MIP-3α and human beta defensins inhibit HIV replication. The inhibition required CCR6 and the induction of APOBEC3G. Here, we further characterize the induction of apolipoprotein B mRNA editing enzyme (APOBEC3G) by human beta defensin 2. Human beta defensin 2 rapidly induces transcriptional induction of APOBEC3G that involves extracellular signal-regulated kinases 1/2 (ERK1/2) activation and the transcription factors NFATc2, NFATc1, and IRF4. We demonstrate that human beta defensin 2 selectively protects primary CCR6+CD4+ T cells infected with HIV-1. The selective protection of CCR6+CD4+ T cell subsets may be critical in maintaining mucosal immune function and preventing disease progression
Handwritten biographical information on Paulina T. McClung Merritt
No full textA handwritten biography of Paulina T. McClung Merritt by an unknown author, 1892.
Heterogeneous and tissue-specific regulation of effector T cell responses by IFN-gamma during Plasmodium berghei ANKA infection.
No full textIFN-γ and T cells are both required for the development of experimental cerebral malaria during Plasmodium berghei ANKA infection. Surprisingly, however, the role of IFN-γ in shaping the effector CD4(+) and CD8(+) T cell response during this infection has not been examined in detail. To address this, we have compared the effector T cell responses in wild-type and IFN-γ(-/-) mice during P. berghei ANKA infection. The expansion of splenic CD4(+) and CD8(+) T cells during P. berghei ANKA infection was unaffected by the absence of IFN-γ, but the contraction phase of the T cell response was significantly attenuated. Splenic T cell activation and effector function were essentially normal in IFN-γ(-/-) mice; however, the migration to, and accumulation of, effector CD4(+) and CD8(+) T cells in the lung, liver, and brain was altered in IFN-γ(-/-) mice. Interestingly, activation and accumulation of T cells in various nonlymphoid organs was differently affected by lack of IFN-γ, suggesting that IFN-γ influences T cell effector function to varying levels in different anatomical locations. Importantly, control of splenic T cell numbers during P. berghei ANKA infection depended on active IFN-γ-dependent environmental signals--leading to T cell apoptosis--rather than upon intrinsic alterations in T cell programming. To our knowledge, this is the first study to fully investigate the role of IFN-γ in modulating T cell function during P. berghei ANKA infection and reveals that IFN-γ is required for efficient contraction of the pool of activated T cells
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