1,720,966 research outputs found
Colture orticole irrigate con acque reflue urbane depurate: aspetti microbiologici e parassitologici (PON In.Te.R.R.A.).
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The importance of being Dientamoeba fragilis
No full textIn recent years, interest in Dientamoeba fragilis on the part of biologists, clinicians and parasitologists has increased considerably due to the need to clarify the many unresolved issues related to the parasite and the associated infection. This paper aims to provide a summary of the state of knowledge of D. fragilis in its different aspects, and, through the analysis of the literature and the most recent data, the present review aims to record the reasons why this protozoan, although little known, should not be underestimated in the diagnosis of human parasitic infections also in Italy
Multiplex PCR for the detection and quantification of key zoonotic genotypes of Giardia, Cryptosporidium and Toxoplasma in wastewater and mussels.
No full textGiardia duodenalis, Cryptosporidium parvum and Toxoplasma gondii are important parasitic protists linked
to water- and food-borne diseases. The accurate detection of these pathogens is central to the diagnosis,
tracking, monitoring and surveillance of these protists in humans, animals and the environment. In this
study, we established a multiplex real-time PCR (qPCR), coupled to high resolution melting (HRM)
analysis, for the specific detection and quantification of each G. duodenalis (assemblage A), C. parvum and
T. gondii (Type I). Once optimised, this assay was applied to the testing of samples (n 1⁄4 232) of treated
wastewater and mussels (Mytilus galloprovincialis). Of 119 water samples, 28.6% were test-positive for
G. duodenalis, C. parvum and/or both pathogens; of 113 mussel samples, 66.6% were test-positive for
G. duodenalis, C. parvum and/or both pathogens, and 13.2% were test-positive for only T. gondii. The
specificity of all amplicons produced was verified by direct sequencing. The oo/cysts numbers (per 5 ml of
DNA sample) ranged from 10 to 64. The present multiplex assay achieved an efficiency of 100% and a R2
value of >0.99. Current evidence indicates that this assay provides a promising tool for the simultaneous
detection and quantitation of three key protist taxa
Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR.
No full textcauses canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sang-uineus group ticks has been only suggested, but definitive evidence is lacking. Thisstudy aimed to detect and quantify A. platys DNA in infected dogs and in their respec-tive ticks through a quantitative real-time PCR assay. From March to May 2009, bloodand tick samples from dogs residing in a CICT-endemic area were collected and molec-ularly analysed. Differences (p 0.05). The mean bacterial load detected in positive dogs was lower than that in theirrespective ticks (p > 0.05). This study provides circumstantial evidence of the putative roleof Rhipicephalus sp. I as a vector of this pathogen
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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