327 research outputs found
Positive regulation of euchromatic gene expression by HP1
No full textHP1 is a conserved prototype protein that plays an essential role in heterochromatin formation and epigenetic gene silencing through its interaction with histone methyltransferase enzymes (HMTases) and the histone H3 at lysine 9 (H3-MeK9). HP1 is also involved in telomere capping and, more surprisingly, in positive regulation of gene expression. Recently, a wide expression analysis, using a RIP-chip assays (RNA-immunoprecipitation on microarrays), has shown that HP1 associates with the transcripts of more than one hundred euchromatic genes and interacts with the heterogeneous nuclear ribonucleoproteins (hnRNPs) that are known to be involved in RNA processing. By these results, HP1 seems to be part of a complex that stabilizes RNA transcripts. Though previously unsuspected, it was also found that HP1-interacting hnRNPs have a functional role in heterochromatin formation. These proteins bind heterochromatin and are dominant suppressors of position effect variegation. Taken together, the results in the paper by Piacentini et al. open a window on a possible new conceptual landscape in which similar epigenetic mechanisms could have a significant role, both in the metabolism of RNA transcripts and in heterochromatin formation, producing opposite functional effects. These data seem to establish a functional link between euchromatin and heterochromatin
Life, works and great discoveries of Victor Loret
No full textMany previously unknown aspects of the life and great discoveries of the French Egyptologist V. Loret are illustrated through unpublished documents kept in the Egyptological Archives of the Università degli Studi di Milano, shown at the exhibition organized by the author in the Cairo Museum. The catalogue is edited by the author as well. The objects discovered by Loret in the Valley of the Kings and in Saqqara, identified in the Cairo Museum, are illustrated in the catalogue and mentioned in the article
"Sind and Hind Proper". The Court Genre in Arabic and the Fathnamah-i Sind. The Sea and its Centrality
No full textPreface
No full textIntroduction to a collection of articles published on EDAL, Egyptian and Egyptological Documents Archives Libraries, edited by the author
Assuan e la sua... città per l'eternità
No full textUpdating on the archaeological excavation in the necropolis of the Aga Khan in Aswan, directed by the author in collaboration with the Egyptian Ministry of Tourism and Antiquities since 2017
Abemaciclib: eventi avversi e riduzione di dose.
No full textLa paziente è una donna di 64 anni, in stato post-menopausale dall’età di 51 anni, in ottime condizioni
cliniche generali (ECOG performance status (PS)=0).
Non riporta significativa familiarità per neoplasie, in particolare per carcinoma mammario e/o ovarico.
In anamnesi: insufficienza renale cronica in stadio III in follow-up nefrologico, ipercolesterolemia con
ateromasia carotidea controllata con statine, intolleranza glucidica in dietoterapia. Dopo oltre cinque
anni dal termine del trattamento chemio e ormonoterapico adiuvante per carcinoma mammario endocrino-
sensibile, le vengono diagnosticate metastasi scheletriche e linfonodali da carcinoma mammario
a fenotipo luminal-A lik
Vitamin C: should daily administration keep the paediatrician away?
No full textVitamin C is an organic compound that is almost ubiquitous in the daily diet of individuals. There are clear indications of supplementation when secondary deficiency is detected related with reduced dietary intake or reduced absorption. On the other hand, indications for supplementation concerning an increased need are controversial. Several authors have studied the role of vitamin C as an adjuvant in the treatment of diseases that may affect children and adolescents. These diseases affect all organs and systems: specifically, vitamin C supplementation could play a role in respiratory, neurological, psychiatric, oncohematological, nephrological, ophthalmological and nutritional disorders. In paediatric age, a significant benefit of vitamin C supplementation has been observed in depressive pathology, iron-deficiency anaemia and chronic renal failure related to haemodialysis. No evidence was found with vitamin C supplementation on mortality, cognitive performance, quality of life, eye diseases, infections, cardiovascular diseases and tumours. This evidence may be related to the fact that in developed countries, vitamin C is almost ubiquitous in the daily diet of each individual. In conclusion, studies on non-industrialised populations in which there could be a real benefit from such supplementation, have yet to be conducted
Heterochromatin protein 1 (HP1) is intrinsically required for post-transcriptional regulation of Drosophila Germline Stem Cell (GSC) maintenance
No full textA very important open question in stem cells regulation is how the fine balance between GSCs self-renewal and differentiation is orchestrated at the molecular level. In the past several years much progress has been made in understanding the molecular mechanisms underlying intrinsic and extrinsic controls of GSC regulation but the complex gene regulatory networks that regulate stem cell behavior are only partially understood. HP1 is a dynamic epigenetic determinant mainly involved in heterochromatin formation, epigenetic gene silencing and telomere maintenance. Furthermore, recent studies have revealed the importance of HP1 in DNA repair, sister chromatid cohesion and, surprisingly, in positive regulation of gene expression. Here, we show that HP1 plays a crucial role in the control of GSC homeostasis in Drosophila. Our findings demonstrate that HP1 is required intrinsically to promote GSC self-renewal and progeny differentiation by directly stabilizing the transcripts of key genes involved in GSCs maintenanc
Retrotransposon activation and genomic instability participate to Huntington Disease pathogenesis in a Drosophila melanogaster model
No full textHuntington's disease (HD) is a late-onset, autosomal dominant disorder characterized by progressive motor dysfunction, early death and psychiatric disturbances. The disease is caused by a CAG repeat expansion in the IT15 gene, which elongates a stretch of polyglutamine (polyQ) at the amino-terminus of the HD protein, huntingtin (Htt). Despite the accumulated data on the molecular basis of
neurodegeneration, no cure is still available. It is therefore important to keep investigating potential previously unnoticed pathways that may be altered in HD and target of therapeutic treatments.
Transposable elements (TEs) are mobile genetic elements that constitute a large fraction of eukaryotic genomes. Retrotransposons represent approximately 40% and 30% of the human and Drosophila genomes. Mounting evidences suggest mammalian L1 elements are normally active during neurogenesis. Interestingly, recent reports show that unregulated activation of TE is associated with
neurodegenerative diseases.
Our experimental results show that retrotransposon transcripts are up-regulated in HD brain and that their inhibition determines the block of polyQ-dependent neurodegeneration. Moreover, we found a high rate of DNA damage and chromosomal abnormalities in HD brains. Taken together, these data suggest that TE activation and genomic instability represent two important pieces in the complicated puzzle of polyQ-induced neurotoxicity
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