1,720,967 research outputs found
Classification and quantitation of 1H NMR spectra of aldithiols binary mixtures using artificial neural networks
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Il diritto penale della medicina nella giurisprudenza di legittimità (2004-2010)
No full textL'opera si offre come una raccolta completa e sistematica della giurisprudenza di legittimità nel periodo 2004-2010 nella materia del diritto penale della medicina Tutte le sentenze, comprensive della motivazione per esteso, sono state raccolte nel CD-Rom allegato al volume Esse sono state riprodotte in modo da consentire la ricerca testuale dei lemmi di interesse Ciascuna pronuncia è preceduta da un lemmario, che ne facilita la consultazione, nonché dalle eventuali massime estrapolabili dalla singola motivazione Queste ultime sono conformi, ove disponibili, alle massime reperibili nel Massimario Ufficiale della Cassazione e, ove edite, alla versione redazionale offertone dalla rivist
Unveiling VIVO2+ Binding Modes to Human Serum Albumins by an Integrated Spectroscopic–Computational Approach
No full textHuman serum albumin (HSA) is involved in the transport of metal ions and potential metallodrugs. Depending on the metal, several sites are available, among which are N-terminal (NTS) and multi-metal binding sites (MBS). Despite the large number of X-ray determinations for albumins, only one structure with Zn2+ is available. In this work, the binding to HSA of the VIVO2+ ion was studied by an integrated approach based on spectroscopic and computational methods, which allowed the systems to be characterized even in the absence of X-ray analysis. The behavior depends on the type of albumin, defatted (HSAd) or fatted (HSAf). With HSAd ‘primary’ and ‘secondary’ sites were revealed, NTS with (His3, His9, Asp13, Asp255) and MBS with (His67, His247, Asp249, Asn99 or H2O); with increasing the ratio VIVO2+/HSAd, ‘tertiary’ sites, with one His-N and other donors (Asp/Glu-O or carbonyl-O) are populated. With HSAf, fatty acids (FAs) cause a rotation of the subdomains IA and IIA, which results in the formation of a dinuclear ferromagnetic adduct (VIVO)2D(HSAf) with a μ1,1-Asp249 and the binding of His247, Glu100, Glu252, and His67 or Asn99. FAs hinder also the binding of VIVO2+ to the MBS
In vitro susceptibility of cell cultures from Echino¬coccus granulosus to anti-microtubular drugs
No full textBiospeciation of Potential Vanadium Drugs of Acetylacetonate in the Presence of Proteins
No full textAmong vanadium compounds with potential medicinal applications, [VIVO(acac)2] is one of the most promising for its antidiabetic and anticancer activity. In the organism, however, interconversion of the oxidation state to +III and +V and binding to proteins are possible. In this report, the transformation of VIII(acac)3, VIVO(acac)2, and VVO2(acac) (Formula presented.) after the interaction with two model proteins, lysozyme (Lyz) and ubiquitin (Ub), was studied with ESI-MS (ElectroSpray Ionization-Mass Spectroscopy), EPR (Electron Paramagnetic Resonance), and computational (docking) techniques. It was shown that, in the metal concentration range close to that found in the organism (15–250 μM), VIII(acac)3 is oxidized to VIVO(acac)+ and VIVO(acac)2, which—in their turn—interact with proteins to give n[VIVO(acac)]–Protein and n[VIVO(acac)2]–Protein adducts. Similarly, the complex in the +IV oxidation state, VIVO(acac)2, dissociates to the mono-chelated species VIVO(acac)+ which binds to Lyz and Ub. Finally, VVO2(acac) (Formula presented.) undergoes complete dissociation to give the 'bare' VVO (Formula presented.) ion that forms adducts n[VVO2]–Protein with n = 1–3. Docking calculations allowed the prediction of the residues involved in the metal binding. The results suggest that only the VIVO complex of acetylacetonate survives in the presence of proteins and that its adducts could be the species responsible of the observed pharmacological activity, suggesting that in these systems VIVO2+ ion should be used in the design of potential vanadium drugs. If VIII or VVO2 potential active complexes had to be designed, the features of the organic ligand must be adequately modulated to obtain species with high redox and thermodynamic stability to prevent oxidation and dissociation
Catalisi foto elettrochimica con polveri di biossido di titanio e idrogenasti, degradazione di inquinanti organici accoppiata con la produzione di idrogeno
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