1,720,986 research outputs found
Set up of an alternative biological method for DSP diagnosis: statistical analysis and evaluation of the correlation "in vivo/in vitro"
No full textAFLATOSSICOSI INDOTTA E RISPOSTA IMMUNITARIA IN SCROFE GRAVIDE: EFFETTI DELLA SUPPLEMENTAZIONE CON VITAMINA A E VITAMINA E.
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Silter cheese, a traditional Italian dairy product: a source of feasible probiotic strains
No full textSilter cheese is a traditional hard cheese, produced in Valcamonica, Brescia, Italy. A total of 426 lactic
strains isolated from Silter were analyzed to determine their probiotic characteristics. 274 out of
426 strains were found to produce bacteriocins against at least one of eight different pathogens
(Salmonella enterica, Listeria monocytogenes, Salmonella derby, Salmonella thyphimurium, Salmonella
napoli, Staphylococcus aureus, E. coli O157:H7, Salmonella enteritidis). In addition, 211 of 274 bactericin-producer
strains adhered to Caco-2 cells and were characterized by RiboPrinter, revealing
predominance of Enterococcus faecalis (26%) and Enterococcus durans-faecium (22%). These findings
suggest that Silter may qualify as an important source of feasible probiotic strains
VALUTAZIONE DEL POTENZIALE MUTAGENO E GENOTOSSICO DI ALCUNE RESINE ODONTOIATRICHE UTILIZZATE IN AMBITO PROTESICO
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Survival of Arcobacter butzleri during production and storage of artisan water buffalo mozzarella cheese
No full textWater buffalo mozzarella cheese (WBMC) is a fresh stretched cheese produced from whole chilled buffalo milk. Although pasteurization of milk and the use of defined starter cultures are recommended, traditional technology involving unpasteurized milk and natural whey cultures is still employed for WBMC production in Italy. The purpose of this study was to assess the behaviour of Arcobacter butzleri during WBMC production and storage under different temperature conditions (5, 10 and 20°C). Raw milk was experimentally inoculated with one reference strain and two isolates of A. butzleri and the count was monitored during WBMC production and storage. The bacterial count of A. butzleri decreased during curd ripening (from 7.83 log CFU/g to 4.14 log CFU/g in about 4 h) and a further decrease (> 4 log CFU/g) was observed at the end of curd stretching. During storage testing, A. butzleri was never detected by direct plating whereas it was recovered from 12 of the total 162 WBMC until the end of storage testing by enrichment. The results revealed that A. butzleri is able to survive during WBMC production and storage at different temperature conditions. Consequently, traditional WBMC produced from raw milk could represent a potential source of Arcobacter infection for human
The efficiency of acidic electrolyzed oxidizing water to reduce spoilage microorganism load in beef
No full textThe aim of this study was to evaluate the efficacy of
acid electrolysed oxidizing water (EOW) in reducing
contamination by spoilage bacteria in bovine meat in
order to determine if the treatment can limit microbial
growth and extend the shelf life of fresh meat. Five
batches of bovine meat were collected. Samples were
treated with EOW, vacuum-packed and analysed to
determine the mesophilic microbial load, count of
Enterobacteriaceae, lactic acid bacteria, Brochothrix
thermosphacta and Pseudomonas spp. during 20 days
of storage at different temperatures (4°, 8°, 12°C).
Comparison between data obtained from the analysis
of untreated and treated samples showed no significant
difference in the samples analysed immediately after
treatment and during storage. Treatment by immersion
of bovine meat cuts for 45 seconds in EOW has shown
to be ineffective in reducing contamination by the
bacteria more frequently involved in meat spoilage
Characterization of endogenous retroviruses in pig cell cultures
Full text linkThe use of pig organs and tissues as alternative source for human transplantation has
been studied for a long time in order to reduce waiting times for transplantation.
However, there is a lot of concern about the widespread application of xenotransplantation, due to the potential risk of transmission of infections from animal tissues to
human recipients. In particular, there is concern about pig endogenous retroviruses
(PERV) after the demonstration of their in vitro transmission to human cells (Patience
et al., 1997). Despite this finding, and lack of PERV infection evidence following
injection or employment of pig organs or cells in humans, the widespread application
of xenotransplantation is related to an improved knowledge of retrovirus characteristics. Three classes of PERVs, referred to as A, B and C, can be typed on the basis of
their variability in the env gene which is also responsible for the host spectrum range.
Previous investigations carried out in our laboratory have shown that all our pig cell
lines were infected by PERV. Furthermore, this infection was detected in primary cells
prepared from tissues of either domestic or feral pigs. It has also been demonstrated
that the infection could be transmitted from some pig cell lines to human cells (Soncini
et al., 2001) according to previous data (Le Tissier et al., 1997). The purpose of this
study was to identify the endogenous retrovirus classes of the infected pig cells,
together with the evaluation of the potential tumorigenicity displayed by some cell
types and the ability of some retroviral compounds to inhibit virus synthesis
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