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Espressione del peptide orexina A e del suo recettore 1 nell'epididimo di alpaca (Vicugna pacos).
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Heart valve cardiomyocytes of mouse embryos express the serotonin transporter SERT
No full textMultiple evidence demonstrate a role for serotonin and its transporter SERT in heart valve development and disease. By utilizing a Cre/loxP system driven by SERT gene expression, we recently demonstrated a regionally restricted distribution of SERT-expressing cells in developing mouse heart. In order to characterize the cell types exhibiting SERT expression within the mouse heart valves at early developmental stages, in this study we performed immunohistochemistry for Islet1 (Isl1) and connexin- 43 (Cx-43) on heart sections from SERTCre/+;ROSA26R embryos previously stained with X-gal. We observed the co-localization of LacZ staining with Isl1 labelling in the outflow tract, the right ventricle and the conal region of E11.5 mouse heart. Cx-43 labelled cells co-localized with LacZ stained cells in the forming atrioventricular valves. These results demonstrate the cardiomyocyte phenotype of SERT-expressing cells in heart valves of the developing mouse heart, thus suggesting an active role of SERT in early heart valve development
Expression of orexin A and its receptor 1 in the rat epididymis.
Full text linkThe hypothalamic peptide orexin A (oxA) derives from the proteolytic cleavage of the precursor molecule
prepro-orexin. It binds with the high affinity G-protein-coupled orexin receptor 1 (OX1R). Here, we report
the detection of oxA and OX1R in the principal cells of the rat caudal epididymis by immunohistochemistry.
Both oxA and OX1R immunolabelling showed cytoplasmic supranuclear localization, filling the apical portion
of the cells. The expression of prepro-orexin and OX1R mRNA transcripts in the rat epididymis was assessed
by reverse-transcriptase polymerase chain reaction, while the presence of both these proteins in the tissue
was confirmed by Western blotting analysis. Our findings provide the evidence for the presence of oxA and
OX1R in the rat epididymis, and demonstrate that both proteins are locally synthesised, thus suggesting a
role for oxA in governing the fertilizing capability of the immature male gamete
Expression of orexin A and its receptor 1 in the rat epididymis
No full textThe hypothalamic peptide orexin A (oxA) derives from the proteolytic cleavage of the precursor molecule prepro-orexin. It binds with the high affinity G-protein-coupled orexin receptor 1 (OX1R). Here, we report the detection of oxA and OX1R in the principal cells of the rat caudal epididymis by immunohistochemistry. Both oxA and OX1R immunolabelling showed cytoplasmic supranuclear localization, filling the apical portion of the cells. The expression of prepro-orexin and OX1R mRNA transcripts in the rat epididymis was assessed by reverse-transcriptase polymerase chain reaction, while the presence of both these proteins in the tissue was confirmed by Western blotting analysis. Our findings provide the evidence for the presence of oxA and OX1R in the rat epididymis, and demonstrate that both proteins are locally synthesised, thus suggesting a role for oxA in governing the fertilizing capability of the immature male gamete
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Novel localization of orexin A in the tubular cytotypes of the rat testis
Full text linkAbstract
The hypothalamic peptides orexin A (OXA) and orexin B (OXB), deriving from the proteolytic cleavage of the precursor molecule prepro-orexin, have also been localized in multiple cerebral areas and peripheral organs. They regulate food intake, arterial blood pressure, heart rate, sleep/wake cycle, sexual behavior, arousal, and the hypothalamic/hypophyseal axes. Prepro-orexin mRNA expression and OXA-immunoreactivity were previously detected in the rat testis at different ages of postnatal development, with strong peptide signal in Leydig cells and spermatocytes. In this study, OXA-immunoreactivity was found in Sertoli cells and spermatids of rat testis. Hematoxylin-counterstained sections revealed OXA positive spermatids in the stages of the germinal epithelium cycle ranging from the VIIth to the XIVth. The expression of prepro-orexin mRNA and of the protein in the testis tissue was ascertained by reverse-transcription polymerase chain reaction and Western blotting analysis, respectively. Although the functional role of OXA in the male genital tract still remains to be elucidated, our findings provide the first evidence that Sertoli cells, belonging to the tubular compartment of testis, represent an important source of OXA, thus suggesting the potential involvement of the peptide in the control of seminiferous epithelium development
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