100,943 research outputs found
Joshua Davis: Author of Spare Parts
No full textCitation: K-State First (2016). Joshua Davis: Author of Spare Parts [Flier]. Manhattan, Kansas: K-State First.Flyer advertising Joshua Davis's author talk at Kansas State University
Steven Johnson Author Talk Poster
No full textK-State Book NetworkA poster advertising an author talk by Steven Johnson at Kansas State University on September 3, 2014. Steven Johnson's book "The Ghost Map" was the 2014-2015 common book
Operation Concept of the Dolní Lipka – Hanušovice Railway Line
No full textPředmětem bakalářské práce „Provozní koncepce trati Dolní Lipka – Hanušovice“ je shrnutí historických souvislostí tratě, analýza přepravních vztahů v území a návrhu alternativní provozní koncepce včetně přestupních vazeb k současné a k návrhové provozní koncepci nynějšího dopravce.The thesis "Operation concept of the Dolní Lipka – Hanušovice Railway Line" is a summary of the historical context of the line, an analysis of transport relations in the area and a proposal of an alternative operational concept including transfer links to the current and to the proposed operational concept of the current carrier
[UMBC Canine patrol dog Rocky with Phil Lipka, Bob Larkins, and Bob Neilson, 1982]
No full textRocky, from the UMBC Canine Patrol, shaking hands with Johns Hopkins University director of public safety, Bob Larkins. UMBC directory of pubic safety, Bob Neilson, and K-9 corps officer, Phil Lipka, sit and watch.On Verso: University of Maryland Baltimore County Canine Patrol-1. Sep 29-1983 M. Sunpapers Photograph Lloyd Pearson Date 9/28/82. Campus [? Meeting. UMBC. L to R. Bob Larkins Dir Public Safety J. H. Univ.; Bob Neilson Dir. Pub. Safety U.M.B.C. Phil Lipka - ""Rocky"" of K-9 corps. On clipping: Police patrol dog Rocky with his handler, Officer Phil Lipka (right); Bob Larkins (left), director of public safety at the Johns Hopkins University, and Bob Neilson, director of public safety at UMBC
The lysin motif receptor-like kinase (LysM-RLK) CERK1 is a major chitin-binding protein in Arabidopsis thaliana and subject to chitin-induced phosphorylation
No full textPlants detect potential pathogens by sensing microbe-associated molecular patterns via pattern recognition receptors. In the dicot model plant Arabidopsis, the lysin motif (LysM)-containing chitin elicitor receptor kinase 1 (CERK1) has been shown to be essential for perception of the fungal cell wall component chitin and for resistance to fungal pathogens. Recent in vitro studies with CERK1 protein expressed heterologously in yeast suggested direct chitin binding activity. Here we show in an affinity purification approach that CERK1 is a major chitin-binding protein of Arabidopsis cells, along with several known and putative chitinases. The ectodomain of CERK1 harbors three distinct LysM domains with potential ligand binding capacity. We demonstrate that the CERK1 ectodomain binds chitin and partially deacetylated chitosan directly without any requirement for interacting proteins and that all three LysM domains are necessary for chitin binding. Ligand-induced phosphorylation events are a general feature of animal and plant signal transduction pathways. Our studies show that chitin, chitin oligomers, and chitosan rapidly induce in vivo phosphorylation of CERK1 at multiple residues in the juxtamembrane and kinase domain. Functional analyses with a kinase dead variant provide evidence that kinase activity of CERK1 is required for its chitin-dependent in vivo phosphorylation, as well as for early defense responses and downstream signaling. Collectively, our data suggest that in Arabidopsis, CERK1 is a major chitin, chitosan, and chito-oligomer binding component and that chitin signaling depends on CERK1 post-translational modification and kinase activity
Live and let die - Arabidopsis nonhost resistance to powdery mildews
No full textThe term "nonhost resistance" (NHR) describes the phenomenon that an entire plant species is resistant to all genetic variants of a non-adapted pathogen species. In nature, NHR represents the most robust form of plant immunity and is therefore of scientific as well as economic importance. Due to its highly complex nature, NHR has previously not been studied in detail. Recently, the establishment of model interaction systems utilizing Arabidopsis and non-adapted powdery mildews allowed the identification of several key components and conceptual conclusions. It is now generally accepted that NHR of Arabidopsis to powdery mildews comprises two distinct layers of defence: pre-invasion entry control at the cell periphery and post-invasion resistance based on cell death execution. The timely production and localised discharge of toxic compounds at sites of fungal attack appear to be pivotal for entry control. This process requires proteins involved in secretion and trans-membrane transport, synthesis and activation of indolic glucosinolates as well as gene regulation and post-translational protein modification. Post-invasion defence relies on lipase-like proteins and salicylic acid signalling. To what extent pathogen-associated molecular pattern- or effector-triggered immunity contribute to NHR remains to be investigated and is likely to depend on the model system studied. (C) 2009 Elsevier GmbH. All rights reserved
Nucleoporin <i>NUP88/MOS7</i> is required for manifestation of phenotypes associated with the Arabidopsis <i>CHITIN ELICITOR RECEPTOR KINASE1</i> mutant <i>cerk1–4</i>
No full textArabidopsis nucleoporin MOS7/NUP88 was identified in a forward-genetic screen for components that contribute to auto-immunity of the deregulated Resistance (R) gene mutant snc1, and is required for immunity to biotrophic and hemi-biotrophic pathogens. In a recent study, we showed that MOS7 is also essential to mount a full defense response against the necrotrophic fungal pathogen Botrytis cinerea, suggesting that MOS7 modulates plant defense responses to different types of pathogenic microbes. Here, we extend our analyses of MOS7-dependent plant immune responses and report the genetic requirement of MOS7 for manifestation of phenotypes associated with the CHITIN ELICITOR RECEPTOR KINASE1 (CERK1) mutant cerk1-4.German Research Foundation (DFG) [WI 3208/4-2
Radionuclides in raised bogs: a case study of Bór za Lasem
No full textBór za Lasem is a raised bog in the Orawsko-Nowotarska Valley (southern Poland). About half of the Bór za Lasem area has been exploited while the rest is undisturbed. Peat samples from both parts of the bog were analysed. Measurements of 137Cs, 134Cs, 239,240Pu, 238Pu ,40K, 228Ra, and 226Ra activity concentrations in all samples were performed. Comparison of the results of radiocaesium and plutonium activity concentrations reveals the highest activity concentrations of radionuclides in the undisturbed part of the peat-bog area. The radiocaesium content decreased with depth, whereas the plutonium concentration had a maximum value at a depth of 15–20 cm. The lowest activity concentrations were found in the exploited part of the bog. Potassium and radium isotopes were detected only in shallow openings within the heavily exploited part of the bog, which might suggest partial mixing of the peat with the mineral sub-soil. The growth rate of the top layer of the undisturbed part of the bog obtained from plutonium distribution was estimated as approximately 0.5 cm per year
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