1,720,963 research outputs found
Dose RACK1A have protein-protein interaction with 14-3-3 protein in Arabidopsis thaliana？
No full textReceptor for Activated C-Kinase 1 (RACK1)是一個分子量為36 kDa的鷹架蛋白，普遍存在於動物和植物中，RACK1的蛋白質結構是由七個平板螺旋 (seven-bladed propeller) 所組成，其中包含七個色胺基酸和天冬胺酸 (tryptophan-aspartic acid-domain；WD40) 的重複區域，而這些重複的區域 (domain) 也是與其他蛋白發生交互作用的地方。在阿拉伯芥中，有三個基因可以轉譯出RACK1蛋白，分別命名為RACK1A (At1g18080), RACK1B (At1g48630) 和RACK1C (At3g18130)。阿拉伯芥中的RACK1蛋白會參與在賀爾蒙反應和植物生長發育的過程中，且會與40S核醣體發生交互作用。目前，RACK1被視為一多功能性的鷹架蛋白，在不同的訊息傳遞途徑中扮演重要的角色。此外，RACK1在離層酸 (abscisic acid; ABA) 反應中扮演一個負向調控的角色，而rack1a突變株對於水分逆境具有抗性。然而，RACK1A究竟是與哪些蛋白質發生交互作用，共同參與或調控這些訊息傳遞的過程，都是尚未清楚的部分。前人研究以14-3-3抗體進行免疫沉澱 (immunoprecipitation) 的實驗，發現到RACK1A的存在。於是，我們想要再進一步去確認14-3-3蛋白與RACK1A蛋白之間的交互作用。由酵母菌雙雜合系統和雙分子螢光互補實驗得知，14-3-3蛋白與RACK1A蛋白之間沒有直接的交互作用。此外，觀察阿拉伯芥RACK1A在原生質體細胞內主要分布在細胞質，C端某絲胺酸定點突變導致RACK1A分布集中在核中。本研究並利用RACK1A大量表現植株，試著將RACK1A及其結合蛋白利用免疫沉澱方式純化出來。Receptor for Activated C-Kinase 1 (RACK1) is a 36-kDa scaffold protein, which contains seven Trp-Asp 40 (WD40) repeats. RACK1 is highly conserved protein in both animals and plants. The WD40 repeats are involved in protein-protein interactions. There are three RACK1 genes in Arabidopsis thaliana, namely RACK1A (At1g18080), RACK1B (At1g48630) and RACK1C (At3g18130). RACK1 is involved in multiple hormone responses, developmental processes, and associated with 40S ribosomes in Arabidopsis. Notably, RACK1 is regarded as a versatile scaffold protein which serves as a nexus for multiple signal transduction pathways. Moreover, RACK1 plays a negative regulator under abscisic acid (ABA) responses, and rack1a mutants are more resistant to water stress. However, the specific interactions between RACK1 and its binding partners are still unclear. Previous immunopreciptation studies revealed that RACK1A may interact with 14-3-3. Here, we used yeast two-hybrid and bimolecular fluorescence complementation to study the interactions between these two proteins. Our results indicated that RACK1A did not interact with 14-3-3 omega directly. Furthermore, we observed that the RACK1A protein is localized to the cytoplasm in Arabidopsis protoplasts. Point mutation of a serine residue located in the C-terminue resulted in subcellular localization change. We use RACK1A overexpression lines to the possibility to identify RACK1A interacting proteins by immunoprecipitation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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